H2O2 inhibits the growth of human pulmonary fibroblast cells by inducing cell death, GSH depletion and G1 phase arrest

Park,Woo  Hyun

doi:10.3892/mmr.2013.1303

April 2013 Volume 7 Issue 4

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April 2013 Volume 7 Issue 4

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Article

H2O2 inhibits the growth of human pulmonary fibroblast cells by inducing cell death, GSH depletion and G1 phase arrest

Authors:
- Woo Hyun Park
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Affiliations: Department of Physiology, Medical School, Research Institute for Endocrine Sciences, Chonbuk National University, Jeonju 561‑180, Republic of Korea
Pages: 1235-1240
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Published online on: February 1, 2013

https://doi.org/10.3892/mmr.2013.1303
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Abstract

Cultured normal human cells are invaluable biological models for mechanistic studies of oxidative stress. Exogenous hydrogen peroxide (H2O2) is often utilized as a representative model of an oxidative stressor. In the present study, the effect of exogenous H2O2 on cell growth and death was evaluated in normal human pulmonary fibroblast (HPF) cells with respect to reactive oxygen species (ROS) and glutathione (GSH) levels. In MTT assays, H2O2 inhibited the growth of HPF cells with an IC50 of ~50 µM at 24 h. DNA flow cytometric analysis indicated that 50-500 µM H2O2 significantly induced G1 phase arrest of the cell cycle. H2O2 induced cell death in the HPF cells, which was accompanied by cleavage of caspase‑3 and loss of mitochondrial membrane potential (MMP; ∆ψm). However, H2O2 was not observed to significantly induce sub‑G1 cells. H2O2 increased superoxide anion (O2•‑) levels from 120 min and increases in ROS, including O2•‑, were also detected at 24 h. H2O2 increased the activity of superoxide dismutase (SOD). H2O2 also induced GSH depletion in HPF cells at 24 h and decreased GSH levels after only 25 min. In conclusion, H2O2 inhibited the growth of HPF cells via apoptosis and/or necrosis as well as G1 phase arrest, which was accompanied by an intracellular increase in ROS levels and the depletion of GSH. The present study provides an important insight into the toxicological effects of exogenous H2O2 on normal HPF cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

H2O2 inhibits the growth of human pulmonary fibroblast cells by inducing cell death, GSH depletion and G1 phase arrest

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