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Article

Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells

  • Authors:
    • Guangyu Zhou
    • Xuesong Su
    • Jianfei Ma
    • Lining Wang
    • Detian Li
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Nephrology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 1336-1342
    |
    Published online on: February 6, 2013
       https://doi.org/10.3892/mmr.2013.1309
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Abstract

High glucose (HG) in peritoneal dialysates has been demonstrated to induce extracellular matrix (ECM) synthesis by peritoneal mesothelial cells (PMCs) and to contribute to peritoneal fibrosis during continuous ambulatory peritoneal dialysis (CAPD). In the present study, we investigated the effects of pioglitazone, a peroxisome proliferator‑activated receptor γ (PPARγ) agonist, on HG‑induced ECM accumulation and the underlying mechanism in rat PMCs (RPMCs). In cultured RPMCs, HG treatment increased the expression of fibronectin (FN), collagen I and plasminogen activation inhibitor‑1 (PAI‑1) at the mRNA and protein levels, while it downregulated the expression of PPARγ in a time‑ and concentration‑dependent manner. Pretreatment with pioglitazone not only decreased the expression of PAI‑1 and matrix proteins (FN and collagen I), but prevented the downregulation of PPARγ in RPMCs under HG conditions. HG treatment activated the nuclear factor‑κB (NF‑κB) and activator protein‑1 (AP‑1) pathways. In addition, the NF‑κB inhibitor, pyrrolidine dithiocarbamate (PDTC), and the AP‑1 inhibitor, SP600125, decreased the protein levels of FN, collagen I and PAI‑1, suggesting a role for the NF‑κB and AP‑1 pathways in the regulation of ECM accumulation induced by HG in RPMCs. Notably, we demonstrated that pretreatment with pioglitazone significantly inhibited HG‑induced NF‑κB and AP‑1 activation. Collectively, these results suggest that pioglitazone inhibits HG‑induced ECM accumulation in RPMCs by increasing PPARγ expression, and by inhibiting the NF‑κB and AP‑1 pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou G, Su X, Ma J, Wang L and Li D: Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells. Mol Med Rep 7: 1336-1342, 2013.
APA
Zhou, G., Su, X., Ma, J., Wang, L., & Li, D. (2013). Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells. Molecular Medicine Reports, 7, 1336-1342. https://doi.org/10.3892/mmr.2013.1309
MLA
Zhou, G., Su, X., Ma, J., Wang, L., Li, D."Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells". Molecular Medicine Reports 7.4 (2013): 1336-1342.
Chicago
Zhou, G., Su, X., Ma, J., Wang, L., Li, D."Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells". Molecular Medicine Reports 7, no. 4 (2013): 1336-1342. https://doi.org/10.3892/mmr.2013.1309
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou G, Su X, Ma J, Wang L and Li D: Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells. Mol Med Rep 7: 1336-1342, 2013.
APA
Zhou, G., Su, X., Ma, J., Wang, L., & Li, D. (2013). Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells. Molecular Medicine Reports, 7, 1336-1342. https://doi.org/10.3892/mmr.2013.1309
MLA
Zhou, G., Su, X., Ma, J., Wang, L., Li, D."Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells". Molecular Medicine Reports 7.4 (2013): 1336-1342.
Chicago
Zhou, G., Su, X., Ma, J., Wang, L., Li, D."Pioglitazone inhibits high glucose‑induced synthesis of extracellular matrix by NF‑κB and AP‑1 pathways in rat peritoneal mesothelial cells". Molecular Medicine Reports 7, no. 4 (2013): 1336-1342. https://doi.org/10.3892/mmr.2013.1309
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