Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April 2013 Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2013 Volume 7 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Mechanism of chronic dietary iron overload‑induced liver damage in mice

  • Authors:
    • Dan Liu
    • Huan He
    • Dong Yin
    • Ailing Que
    • Lei Tang
    • Zhangping Liao
    • Qiren Huang
    • Ming He
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P.R. China, Department of Pharmacology and Molecular Therapeutics, Nanchang University School of Pharmaceutical Science, Nanchang 330006, P.R. China, Jiangxi Provincial Key Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China
  • Pages: 1173-1179
    |
    Published online on: February 8, 2013
       https://doi.org/10.3892/mmr.2013.1316
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Chronic iron overload may result in hepatic fibrosis and even neoplastic transformation due to a burst of reactive oxygen species (ROS). Mitochondria have been proposed to be important in the production of ROS. The purpose of this study was to investigate the role of the mitochondrial permeability transition pore (mPTP) in the burst of ROS, and to clarify the mechanism whereby ROS induced by iron overload results in hepatic damage. It has been demonstrated that when ferrocene‑induced iron‑overloaded mice were fed the cyclosporin A (CsA), a specific inhibitor of the mPTP, diet (10 mg/kg/day) for 50 days, liver‑to‑body weight ratio, serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), ROS production, mitochondrial swelling, loss of mitochondrial membrane potential (Δψ) and hepatocyte apoptosis decreased. However, the total antioxidant status, including superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px) and catalase activities, increased. The protective effect of CsA on the liver of iron‑overloaded mice may be due to inhibition of the ROS burst and a successive antioxidant effect. To the best of our knowledge, these data provide the first support for the theory that ROS‑induced ROS release (RIRR) may be involved in the burst of ROS in the liver and greatly contribute to the hepatic damage initiated by iron overload.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Nahon P, Ganne-Carrié N, Trinchet JC and Beaugrand M: Hepatic iron overload and risk of hepatocellular carcinoma in cirrhosis. Gastroenterol Clin Biol. 34:1–7. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Allen KJ, Gurrin LC, Constantine CC, et al: Iron-overload-related disease in HFE hereditary hemochromatosis. N Engl J Med. 358:221–230. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Asare GA, Kew MC, Mossanda KS, Paterson AC, Siziba K and Kahler-Venter CP: Effects of exogenous antioxidants on dietary iron overload. J Clin Biochem Nutr. 44:85–94. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Pardo Andreu GL, Inada NM, Vercesi AE and Curti C: Uncoupling and oxidative stress in liver mitochondria isolated from rats with acute iron overload. Arch Toxicol. 83:47–53. 2009.PubMed/NCBI

5 

Starkov AA: The role of mitochondria in reactive oxygen species metabolism and signaling. Ann NY Acad Sci. 1147:37–52. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Murphy MP: How mitochondria produce reactive oxygen species. Biochem J. 417:1–13. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Brady NR, Elmore SP, van Beek JJ, Krab K, Courtoy PJ, Hue L and Westerhoff HV: Coordinated behavior of mitochondria in both space and time: a reactive oxygen species-activated wave of mitochondrial depolarization. Biophys J. 87:2022–2034. 2004. View Article : Google Scholar

8 

Gateau-Roesch O, Argaud L and Ovize M: Mitochondrial permeability transition pore and postconditioning. Cardiovasc Res. 70:264–273. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Saotome M, Katoh H, Yaguchi Y, Tanaka T, Urushida T, Satoh H and Hayashi H: Transient opening of mitochondrial permeability transition pore by reactive oxygen species protects myocardium from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 296:H1125–H1132. 2009. View Article : Google Scholar

10 

Halestrap AP: Calcium, mitochondria and reperfusion injury: a pore way to die. Biochem Soc Trans. 34:232–237. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Galaris D, Skiada V and Barbouti A: Redox signaling and cancer: the role of ‘labile’ iron. Cancer Lett. 266:21–29. 2008.

12 

Tjalkens RB, Valerio LG Jr, Awasthi YC and Petersen DR: Association of glutathione S-transferase isozyme-specific induction and lipid peroxidation in two inbred strains of mice subjected to chronic dietary iron overload. Toxicol Appl Pharmacol. 151:174–181. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Galleano M and Puntarulo S: Hepatic chemiluminescence and lipid peroxidation in mild iron overload. Toxicology. 76:27–38. 1992. View Article : Google Scholar : PubMed/NCBI

14 

Brumby PE and Massey V: Determination of nonheme iron, total iron, and copper. Methods Enzymol. 10:463–474. 1967. View Article : Google Scholar

15 

Beavis AD, Brannan RD and Garlid KD: Swelling and contraction of the mitochondrial matrix. I A structural interpretation of the relationship between light scattering and matrix volume. J Biol Chem. 260:13424–13433. 1985.PubMed/NCBI

16 

Emaus RK, Grunwald R and Lemasters JJ: Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochim Biophys Acta. 850:436–448. 1986. View Article : Google Scholar : PubMed/NCBI

17 

Desmots F, Rissel M, Pigeon C, Loyer P, Loréal O and Guillouzo A: Differential effects of iron overload on GST isoform expression in mouse liver and kidney and correlation between GSTA4 induction and overproduction of free radicals. Free Radic Biol Med. 32:93–101. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Okhawa H, Ohishi N and Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95:351–358. 1979. View Article : Google Scholar : PubMed/NCBI

19 

Beauchamp C and Fridovich I: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 44:276–287. 1971. View Article : Google Scholar : PubMed/NCBI

20 

Lawrence RA and Burk RF: Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 71:952–958. 1976. View Article : Google Scholar : PubMed/NCBI

21 

Pedraza-Chaverri J, Granados-Silvestre MD, Medina-Campos ON and Hernández-Pando R: Effect of the in vivo catalase inhibition on aminonucleoside nephrosis. Free Radic Biol Med. 27:245–253. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Ramm GA and Ruddell RG: Iron homeostasis, hepatocellular injury, and fibrogenesis in hemochromatosis: the role of inflammation in a noninflammatory liver disease. Semin Liver Dis. 30:271–287. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Chen J and Chloupková M: Abnormal iron uptake and liver cancer. Cancer Biol Ther. 8:1699–1708. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Xie JR and Yu LN: Cardioprotective effects of cyclosporine A in an in vivo model of myocardial ischemia and reperfusion. Acta Anaesthesiol Scand. 51:909–913. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Halestrap AP, McStay GP and Clarke SJ: The permeability transition pore complex: another view. Biochimie. 84:153–166. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Novgorodov SA and Gudz TI: Permeability transition pore of the inner mitochondrial membrane can operate in two open states with different selectivities. J Bioenerg Biomembr. 28:139–146. 1996. View Article : Google Scholar : PubMed/NCBI

27 

Joza N, Susin SA, Daugas E, et al: Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature. 410:549–554. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Halestrap AP: The mitochondrial permeability transition - a‘pore way for the heart to die. J Clin Basic Cardiol. 5:29–41. 2002.

29 

Galaris D and Pantopoulos K: Oxidative stress and iron homeostasis: mechanistic and health aspects. Crit Rev Clin Lab Sci. 45:1–23. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Van HB, Woshner V and Santos JH: Role of mitochondrial DNA in toxic responses to oxidative stress. DNA Repair (Amst). 5:145–152. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Zorov DB, Juhaszova M and Sollott SJ: Mitochondrial ROS-induced ROS release: an update and review. Biochim Biophys Acta. 1757:509–517. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Brady NR, Hamacher-Brady A, Westerhoff HV and Gottlieb RA: A wave of reactive oxygen species (ROS)-induced ROS release in a sea of excitable mitochondria. Antioxid Redox Signal. 8:1651–1665. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Serviddio G, Bellanti F, Sastre J, Vendemiale G and Altomare E: Targeting mitochondria: a new promising approach for the treatment of liver diseases. Curr Med Chem. 17:2325–2337. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Zorov DB, Filburn CR, Klotz LO, Zweier JL and Sollott SJ: Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes. J Exp Med. 192:1001–1014. 2000. View Article : Google Scholar

35 

Batandier C, Leverve X and Fontaine E: Opening of the mitochondrial permeability transition pore induces reactive oxygen species production at the level of the respiratory chain complex I. J Biol Chem. 279:17197–17204. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu D, He H, Yin D, Que A, Tang L, Liao Z, Huang Q and He M: Mechanism of chronic dietary iron overload‑induced liver damage in mice. Mol Med Rep 7: 1173-1179, 2013.
APA
Liu, D., He, H., Yin, D., Que, A., Tang, L., Liao, Z. ... He, M. (2013). Mechanism of chronic dietary iron overload‑induced liver damage in mice. Molecular Medicine Reports, 7, 1173-1179. https://doi.org/10.3892/mmr.2013.1316
MLA
Liu, D., He, H., Yin, D., Que, A., Tang, L., Liao, Z., Huang, Q., He, M."Mechanism of chronic dietary iron overload‑induced liver damage in mice". Molecular Medicine Reports 7.4 (2013): 1173-1179.
Chicago
Liu, D., He, H., Yin, D., Que, A., Tang, L., Liao, Z., Huang, Q., He, M."Mechanism of chronic dietary iron overload‑induced liver damage in mice". Molecular Medicine Reports 7, no. 4 (2013): 1173-1179. https://doi.org/10.3892/mmr.2013.1316
Copy and paste a formatted citation
x
Spandidos Publications style
Liu D, He H, Yin D, Que A, Tang L, Liao Z, Huang Q and He M: Mechanism of chronic dietary iron overload‑induced liver damage in mice. Mol Med Rep 7: 1173-1179, 2013.
APA
Liu, D., He, H., Yin, D., Que, A., Tang, L., Liao, Z. ... He, M. (2013). Mechanism of chronic dietary iron overload‑induced liver damage in mice. Molecular Medicine Reports, 7, 1173-1179. https://doi.org/10.3892/mmr.2013.1316
MLA
Liu, D., He, H., Yin, D., Que, A., Tang, L., Liao, Z., Huang, Q., He, M."Mechanism of chronic dietary iron overload‑induced liver damage in mice". Molecular Medicine Reports 7.4 (2013): 1173-1179.
Chicago
Liu, D., He, H., Yin, D., Que, A., Tang, L., Liao, Z., Huang, Q., He, M."Mechanism of chronic dietary iron overload‑induced liver damage in mice". Molecular Medicine Reports 7, no. 4 (2013): 1173-1179. https://doi.org/10.3892/mmr.2013.1316
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team