Effects of the downregulation of SnoN expression on HepG2 cell proliferation and apoptosis

Wang,Wenqi; Liu,Changhong; Wang,Yiguo; Cao,Lili

doi:10.3892/mmr.2013.1340

April 2013 Volume 7 Issue 4

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April 2013 Volume 7 Issue 4

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Article

Effects of the downregulation of SnoN expression on HepG2 cell proliferation and apoptosis

Authors:
- Wenqi Wang
- Changhong Liu
- Yiguo Wang
- Lili Cao
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, P.R. China, General Laboratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong 250014, P.R. China
Pages: 1324-1328
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Published online on: February 26, 2013

https://doi.org/10.3892/mmr.2013.1340
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Abstract

Ski‑novel protein (SnoN) is a proto‑oncogene that belongs to the Ski protein family and is involved in regulating processes such as cell proliferation and apoptosis. To investigate the role of SnoN in the proliferation and apoptosis of HepG2 cells, we downregulated its expression by the use of small interfering RNA (siRNA). Three fragments predicted to have RNAi capacity were designed and synthesized as the target siRNAs (siRNA‑A, ‑B and ‑C). Following transfection, inhibition efficiency was detected by reverse transcription PCR (RT‑PCR) and western blot analysis. The siRNA with the optimal inhibition efficiency was used for the cell proliferation and apoptosis analysis. Cell proliferation was analyzed by the Cell Counting Kit‑8 (CCK‑8) and cell apoptosis was investigated by flow cytometry. In our study, all three siRNAs efficiently inhibited SnoN expression, and siRNA‑C demonstrated the optimal inhibition efficiency. We found that following downregulation of SnoN expression, HepG2 cell proliferation was significantly inhibited (P<0.05), while HepG2 cell apoptosis was significantly increased (P<0.05). SnoN‑specific siRNA is capable of effectively inhibiting the expression of SnoN in human HepG2 cells, and the downregulation of SnoN expression induces growth inhibition and apoptosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of the downregulation of SnoN expression on HepG2 cell proliferation and apoptosis

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Abstract

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