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Article

Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions

  • Authors:
    • Qing Mao
    • Cheng-Xi Lin
    • Xiu-Lin Liang
    • Jian-Shu Gao
    • Biao Xu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, P.R. China, Department of Gerontology, The First People's Hospital of Changzhou, The Third Affiliated Hospital of Suzhou University, Changzhou 213000, P.R. China
  • Pages: 1617-1623
    |
    Published online on: February 28, 2013
       https://doi.org/10.3892/mmr.2013.1348
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Abstract

Mesenchymal stem cells (MSCs) transfected by integrin-linked kinase (ILK) transplantation may improve the function and compliance of the post-infarct cardiac ventricle. We investigated the effect of ILK-modified MSC contiditioned medium (ILK-MSC-CM) on the proliferation of cardiac fibroblasts (CFBs) and collagen synthesis in vitro and in vivo. Myocardial infarction (MI)-induced animals received mesenchymal stem cell conditioned medium (MSC-CM), ILK-MSC-CM, or complete medium alone, subepicardially. A group of animals with MI and no other former intervention served as controls. ILK-MSC-CM inhibited CFB proliferation, reduced the gene expression of type I (Col1a1) and type III collagen (Col3a1), tissue inhibitors of metalloproteinase‑1 (TIMP-1) and ‑2 (TIMP-2), α smooth muscle actin (α-SMA), and connective tissue growth factor (CTGF). It also increased the gene expression of matrix metalloproteinase‑2 (MMP‑2) and -9 (MMP‑9), as measured by qRT-PCR. Four weeks after the left anterior descending (LAD) coronary artery ligation, echocardiographic analysis demonstrated preserved cardiac geometry and contractility in the ILK-MSC-CM treated animals. Decreased infarct size and reduced fibrosis were observed in the ILK-MSC-CM group. Overexpression of ILK regulates paracrine actions of MSCs, and ILK-MSC-CM attenuates CFB proliferation and collagen synthesis through paracrine actions in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Mao Q, Lin C, Liang X, Gao J and Xu B: Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions. Mol Med Rep 7: 1617-1623, 2013.
APA
Mao, Q., Lin, C., Liang, X., Gao, J., & Xu, B. (2013). Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions. Molecular Medicine Reports, 7, 1617-1623. https://doi.org/10.3892/mmr.2013.1348
MLA
Mao, Q., Lin, C., Liang, X., Gao, J., Xu, B."Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions". Molecular Medicine Reports 7.5 (2013): 1617-1623.
Chicago
Mao, Q., Lin, C., Liang, X., Gao, J., Xu, B."Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions". Molecular Medicine Reports 7, no. 5 (2013): 1617-1623. https://doi.org/10.3892/mmr.2013.1348
Copy and paste a formatted citation
x
Spandidos Publications style
Mao Q, Lin C, Liang X, Gao J and Xu B: Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions. Mol Med Rep 7: 1617-1623, 2013.
APA
Mao, Q., Lin, C., Liang, X., Gao, J., & Xu, B. (2013). Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions. Molecular Medicine Reports, 7, 1617-1623. https://doi.org/10.3892/mmr.2013.1348
MLA
Mao, Q., Lin, C., Liang, X., Gao, J., Xu, B."Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions". Molecular Medicine Reports 7.5 (2013): 1617-1623.
Chicago
Mao, Q., Lin, C., Liang, X., Gao, J., Xu, B."Mesenchymal stem cells overexpressing integrin-linked kinase attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions". Molecular Medicine Reports 7, no. 5 (2013): 1617-1623. https://doi.org/10.3892/mmr.2013.1348
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