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Article

Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells

  • Authors:
    • Fuqin Guan
    • Haiting Wang
    • Yu Shan
    • Dongmei Zhang
    • Youyi Zhao
    • Yu Chen
    • Qizhi Wang
    • Ming Wang
    • Xu Feng
  • View Affiliations / Copyright

    Affiliations: Jiangsu Center for Research and Development of Medicinal Plants, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu, P.R. China, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu, P.R. China, Dongtai Institute of Tidal Flat, Nanjing Branch of the Chinese Academy of Sciences, Dongtai, Jiangsu, P.R. China
  • Pages: 1585-1590
    |
    Published online on: March 1, 2013
       https://doi.org/10.3892/mmr.2013.1353
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Abstract

In the present study, the antitumor effects of the nor‑Oleanane type triterpene saponin, Bigelovii A, isolated from Salicornia bigelovii Torr, were examined. Bigelovii A was demonstrated to inhibit HL‑60 human acute promyelocytic leukaemia cell growth with an IC50 value of 2.15 µg/ml. In addition, Bigelovii A promoted apoptosis in HL‑60 cells, as shown by apoptotic morphological changes and the hypodiploid cell assay. Apoptotic induction by Bigelovii A was associated with the downregulation of Bcl‑2, the upregulation of Bax and the activation of caspase‑3, as demonstrated by RT‑PCR and western blot analysis. In addition, a lactate dehydrogenase release test indicated that Bigelovii A may exhibit cytotoxic activity by the induction of cell membrane impairment. This study is the first to identify that Bigelovii A exhibits potential antitumor activity and induces marked apoptosis and membrane permeabilisation in HL‑60 cells. Bigelovii A may be a novel candidate for future cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Guan F, Wang H, Shan Y, Zhang D, Zhao Y, Chen Y, Wang Q, Wang M and Feng X: Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells. Mol Med Rep 7: 1585-1590, 2013.
APA
Guan, F., Wang, H., Shan, Y., Zhang, D., Zhao, Y., Chen, Y. ... Feng, X. (2013). Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells. Molecular Medicine Reports, 7, 1585-1590. https://doi.org/10.3892/mmr.2013.1353
MLA
Guan, F., Wang, H., Shan, Y., Zhang, D., Zhao, Y., Chen, Y., Wang, Q., Wang, M., Feng, X."Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells". Molecular Medicine Reports 7.5 (2013): 1585-1590.
Chicago
Guan, F., Wang, H., Shan, Y., Zhang, D., Zhao, Y., Chen, Y., Wang, Q., Wang, M., Feng, X."Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells". Molecular Medicine Reports 7, no. 5 (2013): 1585-1590. https://doi.org/10.3892/mmr.2013.1353
Copy and paste a formatted citation
x
Spandidos Publications style
Guan F, Wang H, Shan Y, Zhang D, Zhao Y, Chen Y, Wang Q, Wang M and Feng X: Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells. Mol Med Rep 7: 1585-1590, 2013.
APA
Guan, F., Wang, H., Shan, Y., Zhang, D., Zhao, Y., Chen, Y. ... Feng, X. (2013). Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells. Molecular Medicine Reports, 7, 1585-1590. https://doi.org/10.3892/mmr.2013.1353
MLA
Guan, F., Wang, H., Shan, Y., Zhang, D., Zhao, Y., Chen, Y., Wang, Q., Wang, M., Feng, X."Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells". Molecular Medicine Reports 7.5 (2013): 1585-1590.
Chicago
Guan, F., Wang, H., Shan, Y., Zhang, D., Zhao, Y., Chen, Y., Wang, Q., Wang, M., Feng, X."Bigelovii A induces apoptosis of HL‑60 human acute promyelocytic leukaemia cells". Molecular Medicine Reports 7, no. 5 (2013): 1585-1590. https://doi.org/10.3892/mmr.2013.1353
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