Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins

Carcoforo,Paolo; Ura,Blendi; Mischiati,Carlo; Squerzanti,Monica; Lanzara,Vincenzo; Cervellati,Carlo; Calza,Roberta; De Laureto,Patrizia   Polverino; Frare,Erica; Portinari,Mattia; Feriotto,Giordana; Lanzara,Serena; Agostinelli,Enzo; Bergamini,Carlo  M.

doi:10.3892/mmr.2013.1375

May 2013 Volume 7 Issue 5

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May 2013 Volume 7 Issue 5

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Article

Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins

Authors:
- Paolo Carcoforo
- Blendi Ura
- Carlo Mischiati
- Monica Squerzanti
- Vincenzo Lanzara
- Carlo Cervellati
- Roberta Calza
- Patrizia Polverino De Laureto
- Erica Frare
- Mattia Portinari
- Giordana Feriotto
- Serena Lanzara
- Enzo Agostinelli
- Carlo M. Bergamini
View Affiliations / Copyright

Affiliations: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy, Institute for Maternal and Child Health, IRCSS Burlo Garofolo, Trieste, Italy, Department of Biomedical Sciences and Specialty Surgery, University of Ferrara, Ferrara, Italy, CRIBI Biotechnology Centre, University of Padova, Padova, Italy, Istituto Pasteur‑Fondazione Cenci Bolognetti, Department of Biochemical Sciences, Sapienza University of Rome and CNR Institute of Molecular Biology and Pathology, Rome, Italy
Pages: 1700-1704
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Published online on: March 15, 2013

https://doi.org/10.3892/mmr.2013.1375
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Abstract

The aim of the present study was to analyze the protein composition of ductal breast carcinoma and the surrounding normal tissue in individual patients using comparative 2D proteomics and mass spectrometry to detect candidate disease biomarkers for diagnosis and prognosis. Samples of normal and cancerous tissue obtained form 28 patients were analyzed. Chaperonins and cytoskeletal proteins predominated among the 11 proteins for which major changes in abundance were detected. Of these 11 proteins with an altered expression, 2 had a decreased expression and 9 had an increased expression. In addition, the abundance of a few cytokeratins was also altered; however, they were not capable of serving as specific circulatory biomarkers. The proteins which we observed to exhibit an altered expression in infiltrating ductal breast carcinoma may be exploited as novel targets for therapeutic interventions or represent novel diagnostic/prognostic markers for the early detection of aggressive tumors, particularly those with multridrug-resistant phenotypes during the earlier stages of the disease.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins

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Abstract

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