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Article

The mechanism through which octreotide inhibits hepatic stellate cell activity

  • Authors:
    • Jingjing Wang
    • Lin Wang
    • Guanhua Song
    • Bo Han
  • View Affiliations / Copyright

    Affiliations: Clinical Test Department of Shandong Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China, Department of Pathology, Shandong University Medical School, Jinan, Shandong 250012, P.R. China, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
  • Pages: 1559-1564
    |
    Published online on: March 20, 2013
       https://doi.org/10.3892/mmr.2013.1385
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Abstract

Hepatic stellate cells (HSCs) are important in the development of liver fibrosis and in the pathogenesis of portal hypertension. Octreotide, an analogue of somatostatin, has been demonstrated to effectively treat fibrosis and portal hypertension; however, its relative mechanism in HSCs remains unknown. LX‑2, the immortalized HSC line, was used to study the mechanism whereby octreotide functions at different concentrations. Real‑time polymerase chain reaction (PCR) and western blot analysis were used to analyze the expression of fibrosis markers and transcription factors following treatment with octreotide. Soluble secreted endothelin‑1 (ET‑1), collagen I and vascular endothelial growth factor (VEGF) were assessed in the supernatants of cultured cells by enzyme-linked immunosorbent assay (ELISA). In the present study, it was shown that octreotide was able to inhibit the proliferative ability of the LX‑2 cells and decrease the expression of transforming growth factor β (TGF‑β), α‑smooth muscle actin (α‑SMA) and smad‑4a. The transcription factors, including c‑Jun and sp‑1, were downregulated in a dose‑dependent manner following treatment with octreotide. The levels of ET‑1 and collagen I in the supernatant decreased significantly in contrast with the normal levels, whereas the levels of VEGF in the LX‑2 cells and the supernatant increased at a high octreotide concentration (10‑5 nM). Octreotide may exert its effects on ET‑1 or other targeting genes in HSCs through the downregulation of c‑Jun and specificity protein 1 (sp‑1), and the increased levels of VEGF may be the reason for the side effects observed at high concentrations of octreotide.
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1 

Arroyo V, Terra C and Ginès P: Advances in the pathogenesis and treatment of type-1 and type-2 hepatorenal syndrome. J Hepatol. 46:935–946. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Friedman SL: Liver fibrosis - from bench to bedside. J Hepatol. 38(Suppl 1): S38–S53. 2003. View Article : Google Scholar

3 

Bartlett ST, Kuo PC, Johnson LB, Lim JW and Schweitzer EJ: Pancreas transplantation at the University of Maryland. Clin Transpl. 271–280. 1996.

4 

Sutherland DE, Gruessner R, Gillingham K, et al: A single institution’s experience with solitary pancreas transplantation: a multivariate analysis of factors leading to improved outcome. Clin Transpl. 141–152. 1991.

5 

Watts SW, Yang P, Banes AK and Baez M: Activation of Erk mitogen-activated protein kinase proteins by vascular serotonin receptors. J Cardiovasc Pharmacol. 38:539–551. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Li T, Weng SG, Leng XS, et al: Effects of 5-hydroxytamine and its antagonists on hepatic stellate cells. Hepatobiliary Pancreat Dis Int. 5:96–100. 2006.PubMed/NCBI

7 

Friedman SL: Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. J Biol Chem. 275:2247–2250. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Xia D, Zhao RQ, Wei XH, Xu QF and Chen J: Developmental patterns of GHr and SS mRNA expression in porcine gastric tissue. World J Gastroenterol. 9:1058–1062. 2003.PubMed/NCBI

9 

Vindeløv SD, Hartoft-Nielsen ML, Rasmussen AK, et al: Interleukin-8 production from human somatotroph adenoma cells is stimulated by interleukin-1β and inhibited by growth hormone releasing hormone and somatostatin. Growth Horm IGF Res. 21:134–139. 2011.PubMed/NCBI

10 

Yao YL, Xu B, Zhang WD and Song YG: Gastrin, somatostatin, and experimental disturbance of the gastrointestinal tract in rats. World J Gastroenterol. 7:399–402. 2001.PubMed/NCBI

11 

Sun FP, Song YG, Cheng W, Zhao T and Yao YL: Gastrin, somatostatin, G and D cells of gastric ulcer in rats. World J Gastroenterol. 8:375–378. 2002.PubMed/NCBI

12 

Li YY: Mechanisms for regulation of gastrin and somatostatin release from isolated rat stomach during gastric distention. World J Gastroenterol. 9:129–133. 2003.PubMed/NCBI

13 

Reynaert H, Thompson MG, Thomas T and Geerts A: Hepatic stellate cells: role in microcirculation and pathophysiology of portal hypertension. Gut. 50:571–581. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Li T, Shi Z and Rockey DC: Preproendothelin-1 expression is negatively regulated by IFNγ during hepatic stellate cell activation. Am J Physiol Gastrointest Liver Physiol. 302:G948–G957. 2012.

15 

Ma J, Zhang L, Han W, et al: Activation of JNK/c-Jun is required for the proliferation, survival, and angiogenesis induced by EET in pulmonary artery endothelial cells. J Lipid Res. 53:1093–1105. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Fuest M, Willim K, MacNelly S, et al: The transcription factor c-Jun protects against sustained hepatic endoplasmic reticulum stress thereby promoting hepatocyte survival. Hepatology. 55:408–418. 2012. View Article : Google Scholar

17 

Reich N, Tomcik M, Zerr P, et al: Jun N-terminal kinase as a potential molecular target for prevention and treatment of dermal fibrosis. Ann Rheum Dis. 71:737–745. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Zhang Y and Yao X: Role of c-Jun N-terminal kinase and p38/activation protein-1 in interleukin-1β-mediated type I collagen synthesis in rat hepatic stellate cells. APMIS. 120:101–107. 2012.PubMed/NCBI

19 

Avouac J, Palumbo K, Tomcik M, et al: Inhibition of activator protein 1 signaling abrogates transforming growth factor β-mediated activation of fibroblasts and prevents experimental fibrosis. Arthritis Rheum. 64:1642–1652. 2012.

20 

Ye Y and Dan Z: All-trans retinoic acid diminishes collagen production in a hepatic stellate cell line via suppression of active protein-1 and c-Jun N-terminal kinase signal. J Huazhong Univ Sci Technolog Med Sci. 30:726–733. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Ling S, Zhou L, Li H, et al: Effects of 17beta-estradiol on growth and apoptosis in human vascular endothelial cells: influence of mechanical strain and tumor necrosis factor-alpha. Steroids. 71:799–808. 2006. View Article : Google Scholar

22 

Evans PC, Taylor ER and Kilshaw PJ: Signaling through CD31 protects endothelial cells from apoptosis. Transplantation. 71:457–460. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Kang Q and Chen A: Curcumin inhibits srebp-2 expression in activated hepatic stellate cells in vitro by reducing the activity of specificity protein-1. Endocrinology. 150:5384–5394. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Asano Y, Ihn H, Yamane K, Kubo M and Tamaki K: Increased expression levels of integrin alphavbeta5 on scleroderma fibroblasts. Am J Pathol. 164:1275–1292. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Walter T, Hommell-Fontaine J, Gouysse G, et al: Effects of somatostatin and octreotide on the interactions between neoplastic gastroenteropancreatic endocrine cells and endothelial cells: a comparison between in vitro and in vivo properties. Neuroendocrinology. 94:200–208. 2011. View Article : Google Scholar

26 

Paternostro C, David E, Novo E and Parola M: Hypoxia, angiogenesis and liver fibrogenesis in the progression of chronic liver diseases. World J Gastroenterol. 16:281–288. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Rosmorduc O: Antiangiogenic therapies in portal hypertension: a breakthrough in hepatology. Gastroenterol Clin Biol. 34:446–449. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Wang L, Song G and Han B: The mechanism through which octreotide inhibits hepatic stellate cell activity. Mol Med Rep 7: 1559-1564, 2013.
APA
Wang, J., Wang, L., Song, G., & Han, B. (2013). The mechanism through which octreotide inhibits hepatic stellate cell activity. Molecular Medicine Reports, 7, 1559-1564. https://doi.org/10.3892/mmr.2013.1385
MLA
Wang, J., Wang, L., Song, G., Han, B."The mechanism through which octreotide inhibits hepatic stellate cell activity". Molecular Medicine Reports 7.5 (2013): 1559-1564.
Chicago
Wang, J., Wang, L., Song, G., Han, B."The mechanism through which octreotide inhibits hepatic stellate cell activity". Molecular Medicine Reports 7, no. 5 (2013): 1559-1564. https://doi.org/10.3892/mmr.2013.1385
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Wang L, Song G and Han B: The mechanism through which octreotide inhibits hepatic stellate cell activity. Mol Med Rep 7: 1559-1564, 2013.
APA
Wang, J., Wang, L., Song, G., & Han, B. (2013). The mechanism through which octreotide inhibits hepatic stellate cell activity. Molecular Medicine Reports, 7, 1559-1564. https://doi.org/10.3892/mmr.2013.1385
MLA
Wang, J., Wang, L., Song, G., Han, B."The mechanism through which octreotide inhibits hepatic stellate cell activity". Molecular Medicine Reports 7.5 (2013): 1559-1564.
Chicago
Wang, J., Wang, L., Song, G., Han, B."The mechanism through which octreotide inhibits hepatic stellate cell activity". Molecular Medicine Reports 7, no. 5 (2013): 1559-1564. https://doi.org/10.3892/mmr.2013.1385
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