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Molecular Medicine Reports
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May 2013 Volume 7 Issue 5

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Article

NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells

  • Authors:
    • Wenzhong Que
    • Saiyuan Li
    • Junmin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology and Rheumatology, The Third Affiliated Hospital of Fujian Medical University, Fuzhou 350003, P.R. China, Department of Pediatrics, The Children’s Hospital of Fuzhou, Fuzhou 350005, P.R. China, Department of Hematology and Rheumatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, P.R. China
  • Pages: 1641-1645
    |
    Published online on: March 26, 2013
       https://doi.org/10.3892/mmr.2013.1394
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Abstract

Bortezomib is commonly used in treating multiple myeloma (MM). However, a number of patients develop resistance to bortezomib over time. Cox-2 is overexpressed in MM cells and contributes to apoptosis resistance and MM development. In the present study, RPMI8226 MM cells were treated with the Cox-2 inhibitor NS-398 to investigate whether it enhanced the effect of bortezomib on MM. The results showed that NS-398 and bortezomib acted synergistically to inhibit growth, arrest the cell cycle at the G1 phase and to induce the apoptosis of MM cells. NS-398 inhibited the NF-κB p65 protein levels and the expression of various NF-κB target genes, including cyclin D1, c-Myc, survivin and Bcl-2. In conclusion, NS-398 enhanced the efficacy of bortezomib against MM cells in vitro and this was associated with the inhibition of NF-κB signaling. These findings suggest that the combined use of NS-398 and bortezomib may constitute a promising novel treatment protocol for MM patients.
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Copy and paste a formatted citation
Spandidos Publications style
Que W, Li S and Chen J: NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells. Mol Med Rep 7: 1641-1645, 2013.
APA
Que, W., Li, S., & Chen, J. (2013). NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells. Molecular Medicine Reports, 7, 1641-1645. https://doi.org/10.3892/mmr.2013.1394
MLA
Que, W., Li, S., Chen, J."NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells". Molecular Medicine Reports 7.5 (2013): 1641-1645.
Chicago
Que, W., Li, S., Chen, J."NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells". Molecular Medicine Reports 7, no. 5 (2013): 1641-1645. https://doi.org/10.3892/mmr.2013.1394
Copy and paste a formatted citation
x
Spandidos Publications style
Que W, Li S and Chen J: NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells. Mol Med Rep 7: 1641-1645, 2013.
APA
Que, W., Li, S., & Chen, J. (2013). NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells. Molecular Medicine Reports, 7, 1641-1645. https://doi.org/10.3892/mmr.2013.1394
MLA
Que, W., Li, S., Chen, J."NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells". Molecular Medicine Reports 7.5 (2013): 1641-1645.
Chicago
Que, W., Li, S., Chen, J."NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells". Molecular Medicine Reports 7, no. 5 (2013): 1641-1645. https://doi.org/10.3892/mmr.2013.1394
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