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August 2013 Volume 8 Issue 2

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Article

miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin

  • Authors:
    • Dong Xu
    • Qingguang Wang
    • Yong An
    • Lijian Xu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210018, P.R. China, Laboratory of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 379-384
    |
    Published online on: May 31, 2013
       https://doi.org/10.3892/mmr.2013.1504
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Abstract

MicroRNAs have emerged as crucial regulators of tumorigenesis. However, the mechanism by which miR‑203 is involved in the pathogenesis of pancreatic cancer (PC) remains elusive. In the present study, PC cell lines were used as an experimental model to investigate the expression and functional role of miR‑203 in PC. miR‑203 mimic virus, miRNA negative control virus and Survivin shRNA virus were transfected into the PC cell line, CFPAC‑1. mRNA and protein levels of Survivin were detected using qPCR and western blot analysis. Proliferation, apoptosis and cell cycle profiles were detected by an MTT assay and flow cytometry. Female BALB/cA‑nu nude mice were used to validate the role of miR‑203 in vivo. The protein levels of Survivin were found to negatively correlate with miR‑203 levels in four PC cell lines. A luciferase assay revealed that Survivin was a direct target of miR‑203. Transfection with miR‑203 mimic inhibited CFPAC‑1 cell proliferation and induced apoptosis and G1 phase cell cycle arrest, similar to knockdown of Survivin. In the in vivo nude mouse model, the downregulation of Survivin by knockdown of Survivin or transfection with miR‑203 mimic inhibited tumor growth. Results of the current study indicate that miR‑203 regulates the proliferation, apoptosis and cell cycle progression of PC cells by targeting Survivin.
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Copy and paste a formatted citation
Spandidos Publications style
Xu D, Wang Q, An Y and Xu L: miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin. Mol Med Rep 8: 379-384, 2013.
APA
Xu, D., Wang, Q., An, Y., & Xu, L. (2013). miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin. Molecular Medicine Reports, 8, 379-384. https://doi.org/10.3892/mmr.2013.1504
MLA
Xu, D., Wang, Q., An, Y., Xu, L."miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin". Molecular Medicine Reports 8.2 (2013): 379-384.
Chicago
Xu, D., Wang, Q., An, Y., Xu, L."miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin". Molecular Medicine Reports 8, no. 2 (2013): 379-384. https://doi.org/10.3892/mmr.2013.1504
Copy and paste a formatted citation
x
Spandidos Publications style
Xu D, Wang Q, An Y and Xu L: miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin. Mol Med Rep 8: 379-384, 2013.
APA
Xu, D., Wang, Q., An, Y., & Xu, L. (2013). miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin. Molecular Medicine Reports, 8, 379-384. https://doi.org/10.3892/mmr.2013.1504
MLA
Xu, D., Wang, Q., An, Y., Xu, L."miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin". Molecular Medicine Reports 8.2 (2013): 379-384.
Chicago
Xu, D., Wang, Q., An, Y., Xu, L."miR‑203 regulates the proliferation, apoptosis and cell cycle progression of pancreatic cancer cells by targeting Survivin". Molecular Medicine Reports 8, no. 2 (2013): 379-384. https://doi.org/10.3892/mmr.2013.1504
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