Bacterial artificial chromosomes (BACs)-on-Beads™ as a diagnostic platform for the rapid aneuploidy screening of products of conception
- Authors:
- Karen L. Sheath
- Lisa Duffy
- Philip Asquith
- Donald R. Love
- Alice M. George
View Affiliations
Affiliations: Diagnostic Genetics, LabPlus, Auckland City Hospital, Auckland 1148, New Zealand
- Published online on: June 13, 2013 https://doi.org/10.3892/mmr.2013.1519
-
Pages:
650-654
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Abstract
The aim of the present study was to evaluate the use of KaryoLite™ bacterial artificial chromosomes (BACs)‑on‑Beads™ (BoBs) technology for the rapid screening of products of conception (POC). Validation and prospective studies were carried out on 85 and 95 patient samples, respectively. Validation studies had previously been analyzed using routine culture and G-banded karyotyping. BoBs resulted in an abnormality detection frequency of 27%, with a failure rate of <3%. The time required for processing was significantly lower compared with that of tissue culture. In conclusion, BoBs technology decreased the failure rate, while increasing the analytical sensitivity compared with G-banded karyotype analysis alone. Additionally, significant cost savings may be achieved with regard to the time of processing and analysis of specimens.
View References
|
1
|
Lomax B, Tang S, Separovic E, et al:
Comparative genomic hybridization in combination with flow
cytometry improves results of cytogenetic analysis of spontaneous
abortions. Am J Hum Genet. 66:1516–1521. 2000. View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Cirigliano V, Voglino G, Ordoñez E, et al:
Rapid prenatal diagnosis of common chromosome aneuploidies by
QF-PCR, results of 9 years of clinical experience. Prenat Diagn.
29:40–49. 2009.PubMed/NCBI
|
|
3
|
Mann K and Ogilvie CM: QF-PCR:
application, overview and review of the literature. Prenat Diagn.
32:309–314. 2012. View
Article : Google Scholar : PubMed/NCBI
|
|
4
|
Donaghue C, Mann K, Docherty Z, Mazzaschi
R, Fear C and Ogilvie C: Combined QF-PCR and MLPA molecular
analysis of miscarriage products: an efficient and robust
alternative to karyotype analysis. Prenat Diagn. 30:133–137. 2010.
View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Willis AS, van den Veyver I and Eng CM:
Multiplex ligation-dependent probe amplification (MLPA) and
prenatal diagnosis. Prenat Diagn. 32:315–320. 2012. View Article : Google Scholar : PubMed/NCBI
|
|
6
|
Gao J, Liu C, Yao F, et al: Array-based
comparative genomic hybridization is more informative than
conventional karyotyping and fluorescence in situ hybridization in
the analysis of first-trimester spontaneous abortion. Mol
Cytogenet. 5:332012. View Article : Google Scholar
|
|
7
|
Reddy UM, Page GP and Saade GR: The role
of DNA microarrays in the evaluation of fetal death. Prenat Diagn.
32:371–375. 2012. View
Article : Google Scholar : PubMed/NCBI
|
|
8
|
Shaffer LG, Coppinger J, Morton SA, et al:
The development of a rapid assay for prenatal testing of common
aneuploidies and microdeletion syndromes. Prenat Diagn. 31:778–787.
2011. View
Article : Google Scholar : PubMed/NCBI
|
|
9
|
Gross SJ, Bajaj K, Garry D, et al: Rapid
and novel prenatal molecular assay for detecting aneuploidies and
microdeletion syndromes. Prenat Diagn. 31:259–266. 2011. View Article : Google Scholar : PubMed/NCBI
|
|
10
|
Vialard F, Simoni G, Aboura A, et al:
Prenatal BACs-on-Beads™: a new technology for rapid detection of
aneuploidies and microdeletions in prenatal diagnosis. Prenat
Diagn. 31:500–508. 2011.
|
|
11
|
Vialard F, Simoni G, Gomes DM, et al:
Prenatal BACs-on-Beads™: the prospective experience of five
prenatal diagnosis laboratories. Prenat Diagn. 32:329–335.
2012.
|
|
12
|
Alkuraya FS, Martin CL and Kimonis VE:
Recurrent miscarriage in a carrier of a balanced cytogenetically
undetectable subtelomeric rearrangement: how many are we missing?
Prenat Diagn. 26:291–293. 2006. View
Article : Google Scholar : PubMed/NCBI
|
