Bacterial artificial chromosomes (BACs)-on-Beads™ as a diagnostic platform for the rapid aneuploidy screening of products of conception

Sheath,Karen  L.; Duffy,Lisa; Asquith,Philip; Love,Donald  R.; George,Alice   M.

doi:10.3892/mmr.2013.1519

August 2013 Volume 8 Issue 2

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August 2013 Volume 8 Issue 2

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Article

Bacterial artificial chromosomes (BACs)-on-Beads™ as a diagnostic platform for the rapid aneuploidy screening of products of conception

Authors:
- Karen L. Sheath
- Lisa Duffy
- Philip Asquith
- Donald R. Love
- Alice M. George
View Affiliations / Copyright

Affiliations: Diagnostic Genetics, LabPlus, Auckland City Hospital, Auckland 1148, New Zealand
Pages: 650-654
|
Published online on: June 13, 2013

https://doi.org/10.3892/mmr.2013.1519
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Abstract

The aim of the present study was to evaluate the use of KaryoLite™ bacterial artificial chromosomes (BACs)‑on‑Beads™ (BoBs) technology for the rapid screening of products of conception (POC). Validation and prospective studies were carried out on 85 and 95 patient samples, respectively. Validation studies had previously been analyzed using routine culture and G-banded karyotyping. BoBs resulted in an abnormality detection frequency of 27%, with a failure rate of <3%. The time required for processing was significantly lower compared with that of tissue culture. In conclusion, BoBs technology decreased the failure rate, while increasing the analytical sensitivity compared with G-banded karyotype analysis alone. Additionally, significant cost savings may be achieved with regard to the time of processing and analysis of specimens.

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1	Lomax B, Tang S, Separovic E, et al: Comparative genomic hybridization in combination with flow cytometry improves results of cytogenetic analysis of spontaneous abortions. Am J Hum Genet. 66:1516–1521. 2000. View Article : Google Scholar : PubMed/NCBI
2	Cirigliano V, Voglino G, Ordoñez E, et al: Rapid prenatal diagnosis of common chromosome aneuploidies by QF-PCR, results of 9 years of clinical experience. Prenat Diagn. 29:40–49. 2009.PubMed/NCBI
3	Mann K and Ogilvie CM: QF-PCR: application, overview and review of the literature. Prenat Diagn. 32:309–314. 2012. View Article : Google Scholar : PubMed/NCBI
4	Donaghue C, Mann K, Docherty Z, Mazzaschi R, Fear C and Ogilvie C: Combined QF-PCR and MLPA molecular analysis of miscarriage products: an efficient and robust alternative to karyotype analysis. Prenat Diagn. 30:133–137. 2010. View Article : Google Scholar : PubMed/NCBI
5	Willis AS, van den Veyver I and Eng CM: Multiplex ligation-dependent probe amplification (MLPA) and prenatal diagnosis. Prenat Diagn. 32:315–320. 2012. View Article : Google Scholar : PubMed/NCBI
6	Gao J, Liu C, Yao F, et al: Array-based comparative genomic hybridization is more informative than conventional karyotyping and fluorescence in situ hybridization in the analysis of first-trimester spontaneous abortion. Mol Cytogenet. 5:332012. View Article : Google Scholar
7	Reddy UM, Page GP and Saade GR: The role of DNA microarrays in the evaluation of fetal death. Prenat Diagn. 32:371–375. 2012. View Article : Google Scholar : PubMed/NCBI
8	Shaffer LG, Coppinger J, Morton SA, et al: The development of a rapid assay for prenatal testing of common aneuploidies and microdeletion syndromes. Prenat Diagn. 31:778–787. 2011. View Article : Google Scholar : PubMed/NCBI
9	Gross SJ, Bajaj K, Garry D, et al: Rapid and novel prenatal molecular assay for detecting aneuploidies and microdeletion syndromes. Prenat Diagn. 31:259–266. 2011. View Article : Google Scholar : PubMed/NCBI
10	Vialard F, Simoni G, Aboura A, et al: Prenatal BACs-on-Beads™: a new technology for rapid detection of aneuploidies and microdeletions in prenatal diagnosis. Prenat Diagn. 31:500–508. 2011.
11	Vialard F, Simoni G, Gomes DM, et al: Prenatal BACs-on-Beads™: the prospective experience of five prenatal diagnosis laboratories. Prenat Diagn. 32:329–335. 2012.
12	Alkuraya FS, Martin CL and Kimonis VE: Recurrent miscarriage in a carrier of a balanced cytogenetically undetectable subtelomeric rearrangement: how many are we missing? Prenat Diagn. 26:291–293. 2006. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Bacterial artificial chromosomes (BACs)-on-Beads™ as a diagnostic platform for the rapid aneuploidy screening of products of conception

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