Analysis of a novel protein in human colorectal adenocarcinoma

Wang,Kaicheng; Chen,Yao

doi:10.3892/mmr.2013.1526

Analysis of a novel protein in human colorectal adenocarcinoma

Authors:
- Kaicheng Wang
- Yao Chen
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Affiliations: Department of Anatomy, Premedical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: June 14, 2013 https://doi.org/10.3892/mmr.2013.1526
Pages: 529-534

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Abstract

Colorectal adenocarcinoma (CRC) is the third most common type of cancer worldwide with a low 5‑year survival rate. The present study aimed to investigate the structure and function of a novel protein identified from human colorectal adenocarcinoma (CRC). A differentially expressed sequence tag (GenBank accession number, ES274081) was collected from GenBank. Bioinformatics tools were employed to obtain the sequence of the full‑length cDNA in order to localize the open reading frame and to predict the protein sequence. Mass spectrometry was used to analyze the structure of this novel protein and western blot analysis was used to confirm the expression of this protein in human CRC tissue samples. The full‑length cDNA was composed of 4,283‑bp nucleotides and the sequence information was obtained (GenBank accession number, NM_001013649). The corresponding protein molecule contained 165 amino acids, with a monoisotopic molecular weight of 18.6033 kDa and an isoelectric point of 8.43, determined by mass spectrometry. The protein structure and its function in adenocarcinoma were further explored. In the present study, a novel protein, which may be involved in nuclear signal transduction, was identified using bioinformatics, mass spectrometry and western blot analysis.

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