In vitro and in vivo effects of short hairpin RNA targeting integrin-linked kinase in prostate cancer cells

Yuan,Yeqing; Xiao,Yunbei; Li,Qing; Liu,Zhenhua; Zhang,Xiaowei; Qin,Caipeng; Xie,Jing; Wang,Xiaofeng; Xu,Tao

doi:10.3892/mmr.2013.1532

August 2013 Volume 8 Issue 2

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August 2013 Volume 8 Issue 2

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Article

In vitro and in vivo effects of short hairpin RNA targeting integrin-linked kinase in prostate cancer cells

Authors:
- Yeqing Yuan
- Yunbei Xiao
- Qing Li
- Zhenhua Liu
- Xiaowei Zhang
- Caipeng Qin
- Jing Xie
- Xiaofeng Wang
- Tao Xu
View Affiliations / Copyright

Affiliations: Department of Urology, Peking University People's Hospital, Beijing 100044, P.R. China, Department of Urology, Wenzhou People's Hospital, Wenzhou 325000, P.R. China
Pages: 419-424
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Published online on: June 19, 2013

https://doi.org/10.3892/mmr.2013.1532
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Abstract

Integrin-linked kinase (ILK) localizes at focal adhesion sites, plays an important role in cell-matrix interactions and is involved in the regulation of tumor cell growth and migration. The aim of the present study was to clarify the functional characterization of ILK in prostate cancer (PCa) cells. ILK was shown to be overexpressed in 57.1% (36/63) of PCa samples and 18.2% (2/11) of benign prostatic hyperplasia (BPH) samples using immunohistochemical analysis. DU145 PCa cells knocked down for ILK were examined using western blot analysis, proliferation assay, flow cytometry and wound healing assay. Depletion of ILK significantly impaired cell growth and motility, induced apoptosis in vitro, and delayed xenograft tumor proliferation in nude mice, which were important for oncogenesis and tumor progression. Western blot analysis showed that Akt activity was attenuated in ILK‑depleted cells compared with the control cells. These results indicate that ILK knockdown attenuates the biological behavior of PCa cells by decreasing Akt activity, demonstrating that ILK is involved in the development and progression of PCa. Thus, ILK is suggested to serve as a potential therapeutic target for PCa.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

In vitro and in vivo effects of short hairpin RNA targeting integrin-linked kinase in prostate cancer cells

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