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Article

Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts

  • Authors:
    • Akira Kondo
    • Haruhiko Tokuda
    • Rie Matsushima-Nishiwaki
    • Kenji  Kato
    • Gen Kuroyanagi
    • Jun Mizutani
    • Muneyoshi Fukuoka
    • Ikuo Wada
    • Osamu Kozawa
    • Takanobu Otsuka
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Mizuho-ku, Nagoya, Japan, Department of Clinical Laboratory, National Hospital for Geriatric Medicine, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu-shi, Gifu, Japan
  • Pages: 691-695
    |
    Published online on: June 19, 2013
       https://doi.org/10.3892/mmr.2013.1533
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Abstract

Heat shock protein 27 (HSP27) also known as heat shock protein β1 (HSPB1) is a member of the family of small heat shock proteins ubiquitously expressed in all tissues. It has previously been demonstrated that HSP27 regulated the synthesis of osteocalcin and interleukin‑6 in osteoblast‑like MC3T3‑E1 cells. In the present study, the effect of HSP27 on basic fibroblast growth factor (FGF‑2)‑stimulated vascular endothelial growth factor (VEGF) synthesis in MC3T3‑E1 cells, was observed. The levels of VEGF release stimulated by FGF‑2 in the HSP27‑overexpressing MC3T3‑E1 cells were significantly lower compared with those in the control cells. In addition, the levels of VEGF release stimulated by FGF-2 in the phosphomimic HSP27-overexpressing cells were significantly higher compared with those in the non‑phosphorylatable HSP27‑overexpressing cells. Furthermore, no significant differences were observed in the FGF‑2‑induced phosphorylation levels of p44/p42 mitogen‑activated protein (MAP) kinase, p38 MAP kinase, stress‑activated protein kinase/c‑Jun N‑terminal kinase (SAPK/JNK) or p70 S6 kinase among the four types of transfected cells. These results suggested that unphosphorylated HSP27 attenuated the FGF‑2‑stimulated VEGF synthesis in osteoblasts.
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1 

Karsenty G and Wagner EF: Reaching a genetic and molecular understanding of skeletal development. Dev Cell. 2:389–406. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Mirams M, Robinson BG, Mason RS and Nelson AE: Bone as a source of FGF23: regulation by phosphate? Bone. 35:1192–1199. 2004.PubMed/NCBI

3 

Barnes GL, Kostenuik PJ, Gerstenfeld LC and Einhorn TA: Growth factor regulation of fracture repair. J Bone Miner Res. 14:1805–1815. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Itoh N and Ornitz DM: Evolution of the Fgf and Fgfr gene families. Trends Genet. 20:563–569. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Suzuki A, Shinoda J, Kanda S, Oiso Y and Kozawa O: Basic fibroblast growth factor stimulates phosphatidylcholine-hydrolyzing phospholipase D in osteoblast-like cells. J Cell Biochem. 63:491–499. 1996. View Article : Google Scholar

6 

Zelzer E and Olsen BR: Multiple roles of vascular endothelial growth factor (VEGF) in skeletal development, growth, and repair. Curr Top Dev Biol. 65:169–187. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Schipani E, Maes C, Carmeliet G and Semenza GL: Regulation of osteogenesis-angiogenesis coupling by HIFs and VEGF. J Bone Miner Res. 24:1347–1353. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Tokuda H, Kozawa O and Uematsu T: Basic fibroblast growth factor stimulates vascular endothelial growth factor release in osteoblasts: divergent regulation by p42/p44 mitogen-activated protein kinase and p38 mitogen-activated protein kinase. J Bone Miner Res. 15:2371–2379. 2000. View Article : Google Scholar

9 

Tokuda H, Hirade K, Wang X, Oiso Y and Kozawa O: Involvement of SAPK/JNK in basic fibroblast growth factor-induced vascular endothelial growth factor release in osteoblasts. J Endocrinol. 177:101–107. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Takai S, Tokuda H, Hanai Y, Harada A, Yasuda E, Matsushima-Nishiwaki R, Kato H, Ogura S, Ohta T and Kozawa O: Negative regulation by p70 S6 kinase of FGF-2-stimulated VEGF release through stress-activated protein kinase/c-Jun N-terminal kinase in osteoblasts. J Bone Miner Res. 22:337–346. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Taylor RP and Benjamin IJ: Small heat shock proteins: a new classification scheme in mammals. J Mol Cell Cardiol. 38:433–444. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Mymrikov EV, Seit-Nebi AS and Gusev NB: Large potentials of small heat shock proteins. Physiol Rev. 91:1123–1159. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Kozawa O, Niwa M, Matsuno H, Ishisaki A, Kato K and Uematsu T: Stimulatory effect of basic fibroblast growth factor on induction of heat shock protein 27 in osteoblasts: role of protein kinase C. Arch Biochem Biophys. 388:237–242. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Kozawa O, Otsuka T, Hatakeyama D, Niwa M, Matsuno H, Ito H, Kato K, Matsui N and Uematsu T: Mechanism of prostaglandin D(2)-stimulated heat shock protein 27 induction in osteoblasts. Cell Signal. 13:535–541. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Hatakeyama D, Kozawa O, Niwa M, Matsuno H, Kato K, Tatematsu N, Shibata T and Uematsu T: Inhibition by adenylyl cyclase-cAMP system of ET-1-induced HSP27 in osteoblasts. Am J Physiol Endocrinol Metab. 281:E1260–E1266. 2001.PubMed/NCBI

16 

Tokuda H, Kozawa O, Niwa M, Matsuno H, Kato K and Uematsu T: Mechanism of prostaglandin E2-stimulated heat shock protein 27 induction in osteoblast-like MC3T3-E1 cells. J Endocrinol. 172:271–281. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Tokuda H, Niwa M, Ishisaki A, Nakajima K, Ito H, Kato K and Kozawa O: Involvement of stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) in prostaglandin F2alpha-induced heat shock protein 27 in osteoblasts. Prostaglandins Leukot Essent Fatty Acids. 70:441–447. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Hayashi K, Takai S, Matsushima-Nishiwaki R, Hanai Y, Kato K, Tokuda H and Kozawa O: (−)-Epigallocatechin gallate reduces transforming growth factor beta-stimulated HSP27 induction through the suppression of stress-activated protein kinase/c-Jun N-terminal kinase in osteoblasts. Life Sci. 82:1012–1017. 2008.

19 

Tiffee JC, Griffin JP and Cooper LF: Immunolocalization of stress proteins and extracellular matrix proteins in the rat tibia. Tissue Cell. 32:141–147. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Leonardi R, Barbato E, Paganelli C and Lo Muzio L: Immunolocalization of heat shock protein 27 in developing jaw bones and tooth germs of human fetuses. Calcif Tissue Int. 75:509–516. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Kostenko S and Moens U: Heat shock protein 27 phosphorylation: kinases, phosphatases, functions and pathology. Cell Mol Life Sci. 66:3289–3307. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Kostenko S, Johannessen M and Moens U: PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5. Cell Signal. 21:712–718. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Takai S, Tokuda H, Yoshida M, Yasuda E, Matsushima-Nishiwaki R, Harada A, Kato K and Kozawa O: Prostaglandin D2 induces the phosphorylation of HSP27 in osteoblasts: function of the MAP kinase superfamily. Prostaglandins Leukot Essent Fatty Acids. 75:61–67. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Kato K, Adachi S, Matsushima-Nishiwaki R, Minamitani C, Natsume H, Katagiri Y, Hirose Y, Mizutani J, Tokuda H, Kozawa O and Otsuka T: Regulation by heat shock protein 27 of osteocalcin synthesis in osteoblasts. Endocrinology. 152:1872–1882. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Kato K, Tokuda H, Mizutani J, Adachi S, Matsushima-Nishiwaki R, Natsume H, Kozawa O and Otsuka T: Role of HSP27 in tumor necrosis factor-α-stimulated interleukin-6 synthesis in osteoblasts. Int J Mol Med. 28:887–893. 2011.

26 

Sudo H, Kodama HA, Amagai Y, Yamamoto S and Kasai S: In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI

27 

Kozawa O, Suzuki A, Tokuda H and Uematsu T: Prostaglandin F2alpha stimulates interleukin-6 synthesis via activation of PKC in osteoblast-like cells. Am J Physiol. 272:E208–E211. 1997.PubMed/NCBI

28 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

29 

Unnanuntana A, Gladnick BP, Donnelly E and Lane JM: The assessment of fracture risk. J Bone Joint Surg Am. 92:743–753. 2010. View Article : Google Scholar

30 

Liu Y, Beredsen AD, Jia S, Lotunum S, Baron R, Ferrara N and Olsen BR: Intracellular VEGF regulates the balance between osteoblast and adipocyte differentiation. J Clin Invest. 122:3101–3113. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kondo A, Tokuda H, Matsushima-Nishiwaki R, Kato K, Kuroyanagi G, Mizutani J, Fukuoka M, Wada I, Kozawa O, Otsuka T, Otsuka T, et al: Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts. Mol Med Rep 8: 691-695, 2013.
APA
Kondo, A., Tokuda, H., Matsushima-Nishiwaki, R., Kato, K., Kuroyanagi, G., Mizutani, J. ... Otsuka, T. (2013). Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts. Molecular Medicine Reports, 8, 691-695. https://doi.org/10.3892/mmr.2013.1533
MLA
Kondo, A., Tokuda, H., Matsushima-Nishiwaki, R., Kato, K., Kuroyanagi, G., Mizutani, J., Fukuoka, M., Wada, I., Kozawa, O., Otsuka, T."Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts". Molecular Medicine Reports 8.2 (2013): 691-695.
Chicago
Kondo, A., Tokuda, H., Matsushima-Nishiwaki, R., Kato, K., Kuroyanagi, G., Mizutani, J., Fukuoka, M., Wada, I., Kozawa, O., Otsuka, T."Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts". Molecular Medicine Reports 8, no. 2 (2013): 691-695. https://doi.org/10.3892/mmr.2013.1533
Copy and paste a formatted citation
x
Spandidos Publications style
Kondo A, Tokuda H, Matsushima-Nishiwaki R, Kato K, Kuroyanagi G, Mizutani J, Fukuoka M, Wada I, Kozawa O, Otsuka T, Otsuka T, et al: Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts. Mol Med Rep 8: 691-695, 2013.
APA
Kondo, A., Tokuda, H., Matsushima-Nishiwaki, R., Kato, K., Kuroyanagi, G., Mizutani, J. ... Otsuka, T. (2013). Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts. Molecular Medicine Reports, 8, 691-695. https://doi.org/10.3892/mmr.2013.1533
MLA
Kondo, A., Tokuda, H., Matsushima-Nishiwaki, R., Kato, K., Kuroyanagi, G., Mizutani, J., Fukuoka, M., Wada, I., Kozawa, O., Otsuka, T."Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts". Molecular Medicine Reports 8.2 (2013): 691-695.
Chicago
Kondo, A., Tokuda, H., Matsushima-Nishiwaki, R., Kato, K., Kuroyanagi, G., Mizutani, J., Fukuoka, M., Wada, I., Kozawa, O., Otsuka, T."Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor‑2‑stimulated vascular endothelial growth factor release in osteoblasts". Molecular Medicine Reports 8, no. 2 (2013): 691-695. https://doi.org/10.3892/mmr.2013.1533
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