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Article

Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide

  • Authors:
    • Rui Huang
    • Jun Zhang
    • Yong Liu
    • Yingying Hao
    • Chenchen Yang
    • Kangkang Wu
    • Shufeng Cao
    • Chao Wu
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Disease, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210008, P.R. China, Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, Department of Infectious Disease, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China
  • Pages: 669-675
    |
    Published online on: June 25, 2013
       https://doi.org/10.3892/mmr.2013.1542
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Abstract

Polysaccharopeptide (PSP) is well known for its immunoregulatory effects. In the present study, the effect of PSP on white blood cell (WBC) count, T lymphocyte subsets, B lymphocytes, Th1/Th2 balance and negative immune regulators was investigated using an immunosuppressed mouse model. The results demonstrated that the WBC count and the absolute number of CD3+CD4+ T cells, CD3+CD8+ T cells and CD3‑CD19+ B cells in the peripheral blood were increased in PSP‑treated groups as compared with the cyclophosphamide (Cy) group. In addition, PSP reduced interleukin (IL)‑4 and GATA binding protein 3 (GATA‑3) mRNA relative expression levels and elevated the ratios of IL‑2/IL‑4 and the transcription factors, T‑box‑containing protein/GATA‑3. The relative mRNA expression levels of the forkhead/winged‑helix transcription factor box protein 3 (Foxp3), programmed death‑1 (PD‑1) and IL‑10 were also downregulated by PSP. These observations indicate that the immunoregulatory effects of PSP are associated with restoration of WBC number, improving the absolute number of T lymphocyte subsets and B lymphocytes, inducing the Th1/Th2 response and downregulating the negative immune regulators, Foxp3, PD‑1 and IL‑10.
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Copy and paste a formatted citation
Spandidos Publications style
Huang R, Zhang J, Liu Y, Hao Y, Yang C, Wu K, Cao S and Wu C: Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide. Mol Med Rep 8: 669-675, 2013.
APA
Huang, R., Zhang, J., Liu, Y., Hao, Y., Yang, C., Wu, K. ... Wu, C. (2013). Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide. Molecular Medicine Reports, 8, 669-675. https://doi.org/10.3892/mmr.2013.1542
MLA
Huang, R., Zhang, J., Liu, Y., Hao, Y., Yang, C., Wu, K., Cao, S., Wu, C."Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide". Molecular Medicine Reports 8.2 (2013): 669-675.
Chicago
Huang, R., Zhang, J., Liu, Y., Hao, Y., Yang, C., Wu, K., Cao, S., Wu, C."Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide". Molecular Medicine Reports 8, no. 2 (2013): 669-675. https://doi.org/10.3892/mmr.2013.1542
Copy and paste a formatted citation
x
Spandidos Publications style
Huang R, Zhang J, Liu Y, Hao Y, Yang C, Wu K, Cao S and Wu C: Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide. Mol Med Rep 8: 669-675, 2013.
APA
Huang, R., Zhang, J., Liu, Y., Hao, Y., Yang, C., Wu, K. ... Wu, C. (2013). Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide. Molecular Medicine Reports, 8, 669-675. https://doi.org/10.3892/mmr.2013.1542
MLA
Huang, R., Zhang, J., Liu, Y., Hao, Y., Yang, C., Wu, K., Cao, S., Wu, C."Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide". Molecular Medicine Reports 8.2 (2013): 669-675.
Chicago
Huang, R., Zhang, J., Liu, Y., Hao, Y., Yang, C., Wu, K., Cao, S., Wu, C."Immunomodulatory effects of polysaccharopeptide in immunosuppressed mice induced by cyclophosphamide". Molecular Medicine Reports 8, no. 2 (2013): 669-675. https://doi.org/10.3892/mmr.2013.1542
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