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Article

1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro

  • Authors:
    • Ying Chen
    • Lei Dong
    • Dong Weng
    • Fangwei Liu
    • Laiyu Song
    • Cuiying Li
    • Wen Tang
    • Jie Chen
  • View Affiliations / Copyright

    Affiliations: Division of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 708-712
    |
    Published online on: June 25, 2013
       https://doi.org/10.3892/mmr.2013.1553
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Abstract

1,3‑β‑glucan is considered a fungal biomarker and exposure to this agent induces lung inflammation. Previous studies have shown that 1,3‑β‑glucan affects Th1 and Th2 immune responses. Interleukin (IL)‑10 and transforming growth factor (TGF)‑β, as typical anti‑inflammatory cytokines, suppress the Th1 immune response. To investigate the effects of 1,3‑β‑glucan on the secretion of cytokines in co‑cultured mouse macrophages and lymphocytes in vitro, mice were exposed to 1,3‑β‑glucan or phosphate‑buffered saline (PBS) by intratracheal instillation. Following extraction and co‑culture of macrophages and lymphocytes, which were treated with or without 1,3‑β‑glucan in vitro, enzyme‑linked immunosorbent assay (ELISA) was used to detect the levels of cytokines and real‑time reverse transcription (RT)‑polymerase chain reaction (PCR) was used to investigate the mRNA expression of forkhead box p3 (Foxp3) in the cells. We showed that 1,3‑β‑glucan exposure in vitro decreased the secretion of Th1 cytokines and increased the secretion of Th2 cytokines in the culture media. Furthermore, 1,3‑β‑glucan exposure in vitro increased the secretion of IL‑10 and TGF‑β in the culture media. According to these results, 1,3‑β‑glucan exposure in vitro is suggested to promote the secretion of anti‑inflammatory cytokines, which may lead to a decrease in the levels of Th1 cytokines and an increase in the levels of Th2 cytokines. 1,3‑β‑Glucan is suggested to induce regulatory lymphocytes, which partly contributes to an increased secretion of anti‑inflammatory cytokines in co‑cultured mouse macrophages and lymphocytes in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Dong L, Weng D, Liu F, Song L, Li C, Tang W and Chen J: 1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro. Mol Med Rep 8: 708-712, 2013.
APA
Chen, Y., Dong, L., Weng, D., Liu, F., Song, L., Li, C. ... Chen, J. (2013). 1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro. Molecular Medicine Reports, 8, 708-712. https://doi.org/10.3892/mmr.2013.1553
MLA
Chen, Y., Dong, L., Weng, D., Liu, F., Song, L., Li, C., Tang, W., Chen, J."1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro". Molecular Medicine Reports 8.2 (2013): 708-712.
Chicago
Chen, Y., Dong, L., Weng, D., Liu, F., Song, L., Li, C., Tang, W., Chen, J."1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro". Molecular Medicine Reports 8, no. 2 (2013): 708-712. https://doi.org/10.3892/mmr.2013.1553
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Dong L, Weng D, Liu F, Song L, Li C, Tang W and Chen J: 1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro. Mol Med Rep 8: 708-712, 2013.
APA
Chen, Y., Dong, L., Weng, D., Liu, F., Song, L., Li, C. ... Chen, J. (2013). 1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro. Molecular Medicine Reports, 8, 708-712. https://doi.org/10.3892/mmr.2013.1553
MLA
Chen, Y., Dong, L., Weng, D., Liu, F., Song, L., Li, C., Tang, W., Chen, J."1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro". Molecular Medicine Reports 8.2 (2013): 708-712.
Chicago
Chen, Y., Dong, L., Weng, D., Liu, F., Song, L., Li, C., Tang, W., Chen, J."1,3‑β‑Glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti‑inflammatory cytokines in vitro". Molecular Medicine Reports 8, no. 2 (2013): 708-712. https://doi.org/10.3892/mmr.2013.1553
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