Houttuynia cordata Thunb inhibits the production of pro‑inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC‑1 human mast cells

Lee,Hee  Joe; Seo,Hye‑Sook; Kim,Gyung‑Jun; Jeon,Chan  Yong; Park,Jong  Hyeong; Jang,Bo‑Hyoung; Park,Sun‑Ju; Shin,Yong‑Cheol; Ko,Seong‑Gyu

doi:10.3892/mmr.2013.1585

Houttuynia cordata Thunb inhibits the production of pro‑inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC‑1 human mast cells

Authors:
- Hee Joe Lee
- Hye‑Sook Seo
- Gyung‑Jun Kim
- Chan Yong Jeon
- Jong Hyeong Park
- Bo‑Hyoung Jang
- Sun‑Ju Park
- Yong‑Cheol Shin
- Seong‑Gyu Ko
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Affiliations: Department of Oriental Medicine, Kyongwon University, Seongnam, Gyeonggi-Do 461‑701, Republic of Korea, Laboratory of Clinical Biology and Pharmacogenomics and Center for Clinical Research and Genomics, Institute of Oriental Medicine, Kyung Hee University, Seoul 130‑701, Republic of Korea
Published online on: July 11, 2013 https://doi.org/10.3892/mmr.2013.1585
Pages: 731-736
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Houttuynia cordata Thunb (HCT) is widely used in oriental medicine as a remedy for inflammation. However, at present there is no explanation for the mechanism by which HCT affects the production of inflammatory cytokines. The current study aimed to determine the effect of an essence extracted from HCT on mast cell‑mediated inflammatory responses. Inflammatory cytokine production induced by phorbol myristate acetate (PMA) plus a calcium ionophore, A23187, was measured in the human mast cell line, HMC‑1, incubated with various concentrations of HCT. TNF‑α, IL‑6 and IL‑8 secreted protein levels were measured using an ELISA assay. TNF‑α, IL‑6 and IL‑8 mRNA levels were measured using RT‑PCR analysis. Nuclear and cytoplasmic proteins were examined by western blot analysis. The NF‑κB promoter activity was examined by luciferase assay. It was observed that HCT inhibited PMA plus A23187‑induced TNF‑α and IL‑6 secretion and reduced the mRNA levels of TNF‑α, IL‑6 and IL‑8. It was also noted that HCT suppressed the induction of NF‑κB activity, inhibited nuclear translocation of NF‑κB and blocked the phosphorylation of IκBα in stimulated HMC‑1 cells. It was concluded that HCT is an inhibitor of NF‑κB and cytokines blocking mast cell‑mediated inflammatory responses. These results indicate that HCT may be used for the treatment of mast cell‑derived allergic inflammatory diseases.

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