Components of Panax notoginseng saponins enhance the cytotoxicity of cisplatin via their effects on gap junctions

Zhang,Cuiling; Tong,Xuhui; Qi,Benquan; Yu,Xiaobing; Dong,Shuying; Zhang,Suzhi; Li,Xiaoming; Yu,Meiling

doi:10.3892/mmr.2013.1597

September 2013 Volume 8 Issue 3

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September 2013 Volume 8 Issue 3

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Article

Components of Panax notoginseng saponins enhance the cytotoxicity of cisplatin via their effects on gap junctions

Authors:
- Cuiling Zhang
- Xuhui Tong
- Benquan Qi
- Xiaobing Yu
- Shuying Dong
- Suzhi Zhang
- Xiaoming Li
- Meiling Yu
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Affiliations: Department of Pharmacology, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Pharmacy, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China, Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China
Pages: 897-902
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Published online on: July 23, 2013

https://doi.org/10.3892/mmr.2013.1597
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Abstract

Previously, Panax notoginseng saponin (PNS)‑induced enhancement of gap junction (GJ) formation or function was observed to be responsible for the increased cytotoxic action of cisplatin. PNS has three constituents, ginsenoside Rg1 and Rb1, and notoginsenoside R1. The active compounds in PNS responsible for enhancing the cytotoxicity of cisplatin remain unknown. Thus, the effects of the main components of PNS on the cytotoxicity of cisplatin were investigated, as well as the correlation with the modulation of GJ function in transfected HeLa cells. The cytotoxicity of cisplatin (0.25‑1 µg/ml) was increased in the presence of GJs. By contrast, the cytotoxicity of cisplatin was decreased when GJs were inhibited by a GJ blocker or by the inhibition of connexin expression. Ginsenoside Rg1 (100 µM) and notoginsenoside R1 (100 µM) were observed to significantly enhance cisplatin cytotoxicity in cells with functional GJs. Ginsenoside Rb1 had no effect on the cytotoxicity of cisplatin in the presence or absence of functional GJs. Cell exposure to ginsenoside Rg1 and notoginsenoside R1 for 4 h led to significant enhancement of a dye‑coupled GJ in a dose‑dependent manner; however, no effect was observed in cells exposed to ginsenoside Rb1. The present results indicate that ginsenoside Rg1 and notoginsenoside R1 are the active compounds responsible for enhancing the cytotoxic action of cisplatin induced by PNS in the presence of functional GJs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Components of Panax notoginseng saponins enhance the cytotoxicity of cisplatin via their effects on gap junctions

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