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Article

The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo

  • Authors:
    • Jihao Zhou
    • Yonghui Li
    • Yushi Yao
    • Lixin Wang
    • Li Gao
    • Xiaoning Gao
    • Xufeng Luo
    • Jingxin Li
    • Mengmeng Jiang
    • Minhang Zhou
    • Lili Wang
    • Li Yu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China
  • Pages: 1549-1555
    |
    Published online on: August 28, 2013
       https://doi.org/10.3892/mmr.2013.1659
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Abstract

Cancer‑testis antigens (CTAs) are a group of tumor‑associated antigens restricted to male germ cells under normal physiological conditions. CTAs are expressed in certain types of tumors and thus are a novel target for immunotherapy. Nuclear RNA export factor 2 (NXF2) is a CTA of which the expression pattern, regulation and clinical significance are unclear. In the present study, following treatment with a demethylating agent, decitabine, NXF2 expression was detected in the majority of the NXF2‑negative acute leukemia cell lines, but not in healthy donor samples. This finding was confirmed by western blot analysis. Eight primary acute leukemia bone marrow samples were treated with decitabine in vitro, and results showed that NXF2 expression was significantly upregulated. In another nine acute myeloid leukemia or myelodysplastic syndrome patients, it was noted that the expression of NXF2 was upregulated in all patients following the first cycle of decitabine, which suggested that NXF2 was activated by decitabine treatment in vivo. Furthermore, NXF2 expression in acute leukemia cells was demonstrated to be regulated by CpG island hypermethylation. To the best of our knowledge, this is the first study to demonstrate that NXF2 is activated by demethylation in acute leukemia cells in vitro and in vivo. NXF2 may therefore serve as a novel target for immunotherapy against acute leukemia.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Li Y, Yao Y, Wang L, Gao L, Gao X, Luo X, Li J, Jiang M, Zhou M, Zhou M, et al: The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo. Mol Med Rep 8: 1549-1555, 2013.
APA
Zhou, J., Li, Y., Yao, Y., Wang, L., Gao, L., Gao, X. ... Yu, L. (2013). The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo. Molecular Medicine Reports, 8, 1549-1555. https://doi.org/10.3892/mmr.2013.1659
MLA
Zhou, J., Li, Y., Yao, Y., Wang, L., Gao, L., Gao, X., Luo, X., Li, J., Jiang, M., Zhou, M., Wang, L., Yu, L."The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo". Molecular Medicine Reports 8.5 (2013): 1549-1555.
Chicago
Zhou, J., Li, Y., Yao, Y., Wang, L., Gao, L., Gao, X., Luo, X., Li, J., Jiang, M., Zhou, M., Wang, L., Yu, L."The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo". Molecular Medicine Reports 8, no. 5 (2013): 1549-1555. https://doi.org/10.3892/mmr.2013.1659
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Li Y, Yao Y, Wang L, Gao L, Gao X, Luo X, Li J, Jiang M, Zhou M, Zhou M, et al: The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo. Mol Med Rep 8: 1549-1555, 2013.
APA
Zhou, J., Li, Y., Yao, Y., Wang, L., Gao, L., Gao, X. ... Yu, L. (2013). The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo. Molecular Medicine Reports, 8, 1549-1555. https://doi.org/10.3892/mmr.2013.1659
MLA
Zhou, J., Li, Y., Yao, Y., Wang, L., Gao, L., Gao, X., Luo, X., Li, J., Jiang, M., Zhou, M., Wang, L., Yu, L."The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo". Molecular Medicine Reports 8.5 (2013): 1549-1555.
Chicago
Zhou, J., Li, Y., Yao, Y., Wang, L., Gao, L., Gao, X., Luo, X., Li, J., Jiang, M., Zhou, M., Wang, L., Yu, L."The cancer‑testis antigen NXF2 is activated by the hypomethylating agent decitabine in acute leukemia cells in vitro and in vivo". Molecular Medicine Reports 8, no. 5 (2013): 1549-1555. https://doi.org/10.3892/mmr.2013.1659
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