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Article

Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway

  • Authors:
    • Tao-Yi Cai
    • Wei Zhu
    • Xiong-Sheng Chen
    • Sheng-Yuan Zhou
    • Lian-Shun Jia
    • Yan-Qing Sun
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China
  • Pages: 1323-1328
    |
    Published online on: September 4, 2013
       https://doi.org/10.3892/mmr.2013.1668
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Abstract

Mesenchymal stem cells (MSCs) differentiate into numerous different cell types and thus have therapeutic potential for tissue engineering, anti‑inflammatory and immunomodulatory purposes. FGF2 may affect the biological behavior of MSCs. MSCs were transduced with either adenovirus‑null vector/green fluorescent protein (GFP) or a vector encoding for the overexpression of FGF2/GFP. The expression of FGF2 was demonstrated to be significantly higher in MSC (FGF2) compared with MSC (vector) by qPCR and western blot analysis. In order to investigate the function of FGF2 in MSCs over time, it was observed that FGF2 stimulates cell proliferation and induces cell differentiation by activating the mitogen‑activated protein kinase (MAPK) signaling pathway. Following blockade of the FGF2‑induced activation of the extracellular signal‑regulated kinase pathway by overexpression of sprouty isoforms, the marker of differentiation markedly decreased. Altogether, the results demonstrated a novel cell biological mechanism that FGF2 differentiates into tenocytes and the MAPK pathway is key in differentiation.
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1 

Rees JD, Wilson AM and Wolman RL: Current concepts in the management of tendon disorders. Rheumatology (Oxford). 45:508–521. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Järvinen TA, Kannus P, Maffulli N and Khan KM: Achilles tendon disorders: etiology and epidemiology. Foot Ankle Clin. 10:255–266. 2005.

3 

Wang JH, Iosifidis MI and Fu FH: Biomechanical basis for tendinopathy. Clin Orthop Relat Res. 443:320–332. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Hoffmann A and Gross G: Tendon and ligament engineering: from cell biology to in vivo application. Regen Med. 1:563–574. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Kuo CK, Marturano JE and Tuan RS: Novel strategies in tendon and ligament tissue engineering: Advanced biomaterials and regeneration motifs. Sports Med Arthrosc Rehabil Ther Technol. 2:202010. View Article : Google Scholar : PubMed/NCBI

6 

Bruder SP, Fink DJ and Caplan AI: Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem. 56:283–294. 1994. View Article : Google Scholar

7 

Jorgensen C, Gordeladze J and Noel D: Tissue engineering through autologous mesenchymal stem cells. Curr Opin Biotechnol. 15:406–410. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ and Andreeff M: Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors. Cancer Res. 62:3603–3608. 2002.PubMed/NCBI

9 

Khakoo AY, Pati S, Anderson SA, et al: Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi’s sarcoma. J Exp Med. 203:1235–1247. 2006.PubMed/NCBI

10 

Wu X, Chen S, Orlando SA, et al: p85alpha regulates osteoblast differentiation by cross-talking with the MAPK pathway. J Biol Chem. 286:13512–13521. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Giuliani N, Bataille R, Mancini C, Lazzaretti M and Barillé S: Myeloma cells induce imbalance in the osteoprotegerin/osteoprotegerin ligand system in the human bone marrow environment. Blood. 98:3527–3533. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Powers CJ, McLeskey SW and Wellstein A: Fibroblast growth factors, their receptors and signaling. Endocr Relat Cancer. 7:165–197. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Yu PJ, Ferrari G, Galloway AC, Mignatti P and Pintucci G: Basic fibroblast growth factor (FGF-2): the high molecular weight forms come of age. J Cell Biochem. 100:1100–1108. 2007. View Article : Google Scholar : PubMed/NCBI

14 

van den Bos C, Mosca JD, Winkles J, Kerrigan L, Burgess WH and Marshak DR: Human mesenchymal stem cells respond to fibroblast growth factors. Hum Cell. 10:45–50. 1997.PubMed/NCBI

15 

Tsutsumi S, Shimazu A, Miyazaki K, et al: Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun. 288:413–419. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Bianchi G, Banfi A, Mastrogiacomo M, et al: Ex vivo enrichment of mesenchymal cell progenitors by fibroblast growth factor 2. Exp Cell Res. 287:98–105. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Baddoo M, Hill K, Wilkinson R, et al: Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. J Cell Biochem. 89:1235–1249. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Choi SC, Kim SJ, Choi JH, Park CY, Shim WJ and Lim DS: Fibroblast growth factor-2 and -4 promote the proliferation of bone marrow mesenchymal stem cells by the activation of the PI3K-Akt and ERK1/2 signaling pathways. Stem Cells Dev. 17:725–736. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Masola V, Gambaro G, Tibaldi E, et al: Heparanase and syndecan-1 interplay orchestrates fibroblast growth factor-2-induced epithelial-mesenchymal transition in renal tubular cells. J Biol Chem. 287:1478–1488. 2012. View Article : Google Scholar

20 

Solchaga LA, Penick K, Porter JD, Goldberg VM, Caplan AI and Welter JF: FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells. J Cell Physiol. 203:398–409. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Varas L, Ohlsson LB, Honeth G, et al: Alpha10 integrin expression is up-regulated on fibroblast growth factor-2-treated mesenchymal stem cells with improved chondrogenic differentiation potential. Stem Cells Dev. 16:965–978. 2007. View Article : Google Scholar

22 

Neubauer M, Fischbach C, Bauer-Kreisel P, et al: Basic fibroblast growth factor enhances PPARgamma ligand-induced adipogenesis of mesenchymal stem cells. FEBS Lett. 577:277–283. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Lai WT, Krishnappa V and Phinney DG: Fibroblast growth factor 2 (Fgf2) inhibits differentiation of mesenchymal stem cells by inducing Twist2 and Spry4, blocking extracellular regulated kinase activation, and altering Fgf receptor expression levels. Stem Cells. 29:1102–1111. 2011. View Article : Google Scholar

24 

Frederick JP, Liberati NT, Waddell DS, Shi Y and Wang XF: Transforming growth factor beta-mediated transcriptional repression of c-myc is dependent on direct binding of Smad3 to a novel repressive Smad binding element. Mol Cell Biol. 24:2546–2559. 2004. View Article : Google Scholar

25 

Wang L, Blouin V, Brument N, Bello-Roufai M and Francois A: Production and purification of recombinant adeno-associated vectors. Methods Mol Biol. 807:361–404. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Lanner F and Rossant J: The role of FGF/Erk signaling in pluripotent cells. Development. 137:3351–3360. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Ozaki K, Miyazaki S, Tanimura S and Kohno M: Efficient suppression of FGF-2-induced ERK activation by the cooperative interaction among mammalian Sprouty isoforms. J Cell Sci. 118:5861–5871. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cai T, Zhu W, Chen X, Zhou S, Jia L and Sun Y: Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Mol Med Rep 8: 1323-1328, 2013.
APA
Cai, T., Zhu, W., Chen, X., Zhou, S., Jia, L., & Sun, Y. (2013). Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Molecular Medicine Reports, 8, 1323-1328. https://doi.org/10.3892/mmr.2013.1668
MLA
Cai, T., Zhu, W., Chen, X., Zhou, S., Jia, L., Sun, Y."Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway". Molecular Medicine Reports 8.5 (2013): 1323-1328.
Chicago
Cai, T., Zhu, W., Chen, X., Zhou, S., Jia, L., Sun, Y."Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway". Molecular Medicine Reports 8, no. 5 (2013): 1323-1328. https://doi.org/10.3892/mmr.2013.1668
Copy and paste a formatted citation
x
Spandidos Publications style
Cai T, Zhu W, Chen X, Zhou S, Jia L and Sun Y: Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Mol Med Rep 8: 1323-1328, 2013.
APA
Cai, T., Zhu, W., Chen, X., Zhou, S., Jia, L., & Sun, Y. (2013). Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Molecular Medicine Reports, 8, 1323-1328. https://doi.org/10.3892/mmr.2013.1668
MLA
Cai, T., Zhu, W., Chen, X., Zhou, S., Jia, L., Sun, Y."Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway". Molecular Medicine Reports 8.5 (2013): 1323-1328.
Chicago
Cai, T., Zhu, W., Chen, X., Zhou, S., Jia, L., Sun, Y."Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway". Molecular Medicine Reports 8, no. 5 (2013): 1323-1328. https://doi.org/10.3892/mmr.2013.1668
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