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Article

Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy

  • Authors:
    • Qiao‑Zhi Yin
    • Hua Lu
    • Li‑Min Li
    • Shang‑Mian Yie
    • Xiang Hu
    • Zhi‑Bin Liu
    • Xiao Zheng
    • Sheng Cao
    • Zou‑Ying Yao
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Second Medical College/Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610048, P.R. China, Sichuan Academy of Chinese Medicine Sciences, Chengdu, Sichuan 610041, P.R. China, Key Laboratory of Bio‑resources and Eco‑environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu, Sichuan 610064, P.R. China
  • Pages: 1329-1336
    |
    Published online on: September 6, 2013
       https://doi.org/10.3892/mmr.2013.1670
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Abstract

The administration of You Gui Wan (YGW) decoction has been observed to improve vaginal atrophy induced by ovariectomy (OVX) in rats. The aim of the current study was to explore the possible mechanisms underlying this effect. Following OVX, 37 Sprague Dawley female rats were randomly divided into three groups which were orally administered with YGW decoction, saline or estrogen for 11 weeks. In parallel with this, 19 normal and 17 rats with sham‑surgery were used as controls. The effects of these treatments on estrogen receptors (ER) and various angiogenic factors, including vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor‑1 (VEGFR‑1), angiopoietin (Ang)1 and 2 and basic fibroblast growth factor (bFGF) in the vagina were compared using immunohistochemistry or quantitative polymerase chain reaction (qPCR). OVX was found to induce significant vaginal atrophy and decrease the expression of ER and various angiogenic factors when compared with the normal and sham‑surgery animals (all P<0.05). Estrogen replacement and the administration of YGW decoction reversed the vaginal atrophic process. The hormonal replacement and YGW treatment recovered the protein expression of ER‑α and ‑β, VEGF and VEGFR‑1 and the mRNA levels of ER‑α, VEGF, VEGFR‑1, Ang1 and 2, and bFGF when compared with OVX‑rats with saline, normal and sham‑surgery treatments (all P<0.05). Thus, it may be concluded that a possible mechanism underlying the effect of YGW on OVX‑induced vaginal atrophy may be the upregulated expression of ER and various angiogenic factors in the vaginal tissue.
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1 

Anderson GL, Limacher M, Assaf AR, et al: Effects of conjugated equine estrogen in postmenopausal women with hysterectomy: the Women’s Health Initiative randomized controlled trial. JAMA. 291:1701–1712. 2004.

2 

Haines CJ, Lam PA, Chung TK, Cheng KF and Leung PC: A randomized, double-blind, placebo-controlled study of the effect of a Chinese herbal medicine preparation (Dang Gui Buxue Tang) on menopausal symptoms in Hong Kong Chinese women. Climacteric. 11:244–251. 2008. View Article : Google Scholar

3 

Chan CC, Lau WN, Chiu SP, Chen LC, Choi WK and Tang GW: A pilot study on the effects of a Chinese herbal preparation on menopausal symptoms. Gynecol Endocrinol. 22:70–73. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Kwee SH, Tan HH, Marsman A and Wauters C: The effect of Chinese herbal medicines (CHM) on menopausal symptoms compared to hormone replacement therapy (HRT) and placebo. Maturitas. 58:83–90. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Zell B, Hirata J, Marcus A, Ettinger B, Pressman A and Ettinger KM: Diagnosis of symptomatic postmenopausal women by traditional Chinese medicine practitioners. Menopause. 7:129–134. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Hu X, Wang J, Yin QZ, Lu H and Yie SM: You Gui Wan can reverse atrophic effect of ovariectomy on rat vaginal fold and blood vessels in the lamina propria. Biol Pharm Bull. 34:1808–1814. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Applanat MP, Buteau-Lozano H, Herve MA and Corpet A: Vascular endothelial growth factor is a target gene for estrogen receptor and contributes to breast cancer progression. Adv Exp Med Biol. 617:437–444. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Fujimoto J, Hori M, Ichigo S and Tamaya T: Ovarian steroids regulate the expression of basic fibroblast growth factor and its mRNA in fibroblasts derived from uterine endometrium. Ann Clin Biochem. 34:91–96. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Presta M: Sex hormones modulate the synthesis of basic fibroblast growth factor in human endometrial adenocarcinoma cells: implications for the neovascularization of normal and neoplastic endometrium. J Cell Physiol. 137:593–597. 1988. View Article : Google Scholar

10 

Hangai M, Murata T, Miyawaki N, Spee C, Lim JI, He S, Hinton DR and Ryan SJ: Angiopoietin-1 upregulation by vascular endothelial growth factor in human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 42:1617–1625. 2001.PubMed/NCBI

11 

Fiedler U and Augustin HG: Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27:552–558. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Zhou J and Qu F: Treating gynaecological disorders with traditional Chinese medicine: a review. Afr J Tradit Complement Altern Med. 6:494–517. 2009.PubMed/NCBI

13 

Sze SC, Tong Y, Zhang YB, Zhang ZJ, Lau AS, Wong HK, Tsang KW and Ng TB: A novel mechanism: Erxian Decoction, a Chinese medicine formula, for relieving menopausal syndrome. J Ethnopharmacol. 123:27–33. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Becker JB, Arnold AP, Berkley KJ, Blaustein JD, Eckel LA, Hampson E, Herman JP, Marts S, Sadee W, Steiner M, et al: Strategies and methods for research on sex differences in brain and behavior. Endocrinology. 146:1650–1673. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Oberholzer M, Ostreicher M, Christen H and Brühlmann M: Methods in quantitative image analysis. Histochem Cell Biol. 105:333–355. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Yie SM, Li LH, Li GM, Xiao R and Librach CL: Progesterone enhances HLA-G gene expression in JEG-3 choriocarcinoma cells and human cytotrophoblasts in vitro. Hum Reprod. 21:46–51. 2006.PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta DeltaC(T)) Method. Methods. 25:402–408. 2001.

18 

Masood DE, Roach EC, Beauregard KG and Khalil RA: Impact of sex hormone metabolism on the vascular effects of menopausal hormone therapy in cardiovascular disease. Curr Drug Metab. 11:693–714. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Losordo DW and Isner JM: Estrogen and angiogenesis: A review. Arterioscler Thromb Vasc Biol. 21:6–12. 2001. View Article : Google Scholar

20 

Kim KH, Moriarty K and Bender JR: Vascular cell signaling by membrane estrogen receptors. Steroids. 73:864–869. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Kim-Schulze S, McGowan KA, Hubchak SC, Cid MC, Martin MB, Kleinman HK, Greene GL and Schnaper HW: Expression of an estrogen receptor by human coronary artery and umbilical vein endothelial cells. Circulation. 94:1402–1407. 1996. View Article : Google Scholar : PubMed/NCBI

22 

Liu WX, Lu YW, Du HT and Wu ZZ: Pharmacological actions of Radix Rehmanniae and its active components: research advances. J Int Pharm Res. 36:277–280. 2009.

23 

Wang SF, Chen XG, Hu ZD and Ju Y: Analysis of three effective components in Fructus corni and its preparations by micellar electrokinetic capillary chromatography. Biomed Chromatogr. 17:306–311. 2003.PubMed/NCBI

24 

Li SY, Yang D, Yeung CM, Yu WY, Chang RC, So KF, Wong D and Lo AC: Lycium barbarum polysaccharides reduce neuronal damage, blood-retinal barrier disruption and oxidative stress in retinal ischemia/reperfusion injury. PLoS One. 6:e163802011. View Article : Google Scholar

25 

Jin X, Li J and Yan M: Flavonoids in the seed of Cuscuta chinensis Lam. Zhongguo Zhong Yao Za Zhi. 17:292–294. 1992.(In Chinese).

26 

Deyama T, Nishibe S and Nakazawa Y: Constituents and pharmacological effects of Eucommia and Siberian ginseng. Acta Pharmacol Sin. 22:1057–1070. 2001.PubMed/NCBI

27 

Yi L, Liang Y, Wu H and Yuan D: The analysis of Radix Angelicae Sinensis (Danggui). J Chromatogr A. 1216:1991–2001. 2009.

28 

Lockwood GB: Phenylpropanoids from a Nigerian sample of Cinnamomum cassia [proceedings]. J Pharm Pharmacol. 31:8P1979. View Article : Google Scholar

29 

Xie Y, Zhou H, Wong YF, Liu Z, Xu H, Jiang Z and Liu L: An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in Radix Aconiti Lateralis Preparata (Fuzi, aconite roots) and its products. Chin Med. 3:62008. View Article : Google Scholar : PubMed/NCBI

30 

Matsuda H, Shimoda H, Morikawa T and Yoshikawa M: Phytoestrogens from the roots of Polygonum cuspidatum (Polygonaceae): structure-requirement of hydroxyanthraquinones for estrogenic activity. Bioorg Med Chem Lett. 11:1839–1842. 2001.

31 

Valachovicova T, Slivova V and Sliva D: Cellular and physiological effects of soy flavonoids. Mini Rev Med Chem. 4:881–887. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Kostelac D, Rechkemmer G and Briviba K: Phytoestrogens modulate binding response of estrogen receptors alpha and beta to the estrogen response element. J Agric Food Chem. 51:7632–7635. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Xu HQ, Hao HP, Zhang X and Pan Y: Morroniside protects cultured human umbilical vein endothelial cells from damage by high ambient glucose. Acta Pharmacol Sin. 25:412–415. 2004.PubMed/NCBI

34 

Zhang K, Lu J, Mori T, Smith-Powell L, Synold TW, Chen S and Wen W: Baicalin increases VEGF expression and angiogenesis by activating the ERR alpha/PGC-1alpha pathway. Cardiovasc Res. 89:426–435. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yin QZ, Lu H, Li LM, Yie SM, Hu X, Liu ZB, Zheng X, Cao S and Yao ZY: Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy. Mol Med Rep 8: 1329-1336, 2013.
APA
Yin, Q., Lu, H., Li, L., Yie, S., Hu, X., Liu, Z. ... Yao, Z. (2013). Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy. Molecular Medicine Reports, 8, 1329-1336. https://doi.org/10.3892/mmr.2013.1670
MLA
Yin, Q., Lu, H., Li, L., Yie, S., Hu, X., Liu, Z., Zheng, X., Cao, S., Yao, Z."Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy". Molecular Medicine Reports 8.5 (2013): 1329-1336.
Chicago
Yin, Q., Lu, H., Li, L., Yie, S., Hu, X., Liu, Z., Zheng, X., Cao, S., Yao, Z."Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy". Molecular Medicine Reports 8, no. 5 (2013): 1329-1336. https://doi.org/10.3892/mmr.2013.1670
Copy and paste a formatted citation
x
Spandidos Publications style
Yin QZ, Lu H, Li LM, Yie SM, Hu X, Liu ZB, Zheng X, Cao S and Yao ZY: Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy. Mol Med Rep 8: 1329-1336, 2013.
APA
Yin, Q., Lu, H., Li, L., Yie, S., Hu, X., Liu, Z. ... Yao, Z. (2013). Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy. Molecular Medicine Reports, 8, 1329-1336. https://doi.org/10.3892/mmr.2013.1670
MLA
Yin, Q., Lu, H., Li, L., Yie, S., Hu, X., Liu, Z., Zheng, X., Cao, S., Yao, Z."Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy". Molecular Medicine Reports 8.5 (2013): 1329-1336.
Chicago
Yin, Q., Lu, H., Li, L., Yie, S., Hu, X., Liu, Z., Zheng, X., Cao, S., Yao, Z."Impacts of You Gui Wan on the expression of estrogen receptors and angiogenic factors in OVX‑rat vagina: A possible mechanism for the trophic effect of the formula on OVX‑induced vaginal atrophy". Molecular Medicine Reports 8, no. 5 (2013): 1329-1336. https://doi.org/10.3892/mmr.2013.1670
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