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Article

Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers

  • Authors:
    • Ju Long
    • Xuehe Ye
    • Xunjin Weng
    • Kepeng Fu
    • Lei Sun
    • Wanrong Pang
  • View Affiliations / Copyright

    Affiliations: Laboratory of Medical Genetics, Maternal and Child Health Care Hospital of Qinzhou, Guangxi 535099, P.R. China
  • Pages: 1601-1605
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/mmr.2013.1678
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Abstract

The present study aimed to develop a rapid diagnostic test of aneuploidy in chromosomes 13, 18, 21, X and Y through a program combining short tandem repeat (STR) typing with fluorescence-labeled homologous gene quantitative‑polymerase chain reaction (fHGQ-PCR), which avoids misjudgment risks by using one method alone. Furthermore, fluorescently labeled universal primers not only ensure the accuracy of the results but also reduces the cost of fluorescent labels. The verification of DNA extracted from samples confirmed by karyotype analysis with quantitative fluorescence (QF)-PCR shows that the results obtained using the QF-PCR program are consistent with the results of karyotype analysis in rapidly diagnosing the aneuploidy of chromosomes 13, 18, 21, X and Y.
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Copy and paste a formatted citation
Spandidos Publications style
Long J, Ye X, Weng X, Fu K, Sun L and Pang W: Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers. Mol Med Rep 8: 1601-1605, 2013.
APA
Long, J., Ye, X., Weng, X., Fu, K., Sun, L., & Pang, W. (2013). Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers. Molecular Medicine Reports, 8, 1601-1605. https://doi.org/10.3892/mmr.2013.1678
MLA
Long, J., Ye, X., Weng, X., Fu, K., Sun, L., Pang, W."Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers". Molecular Medicine Reports 8.5 (2013): 1601-1605.
Chicago
Long, J., Ye, X., Weng, X., Fu, K., Sun, L., Pang, W."Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers". Molecular Medicine Reports 8, no. 5 (2013): 1601-1605. https://doi.org/10.3892/mmr.2013.1678
Copy and paste a formatted citation
x
Spandidos Publications style
Long J, Ye X, Weng X, Fu K, Sun L and Pang W: Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers. Mol Med Rep 8: 1601-1605, 2013.
APA
Long, J., Ye, X., Weng, X., Fu, K., Sun, L., & Pang, W. (2013). Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers. Molecular Medicine Reports, 8, 1601-1605. https://doi.org/10.3892/mmr.2013.1678
MLA
Long, J., Ye, X., Weng, X., Fu, K., Sun, L., Pang, W."Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers". Molecular Medicine Reports 8.5 (2013): 1601-1605.
Chicago
Long, J., Ye, X., Weng, X., Fu, K., Sun, L., Pang, W."Rapid diagnosis of aneuploidy in chromosomes 13, 18, 21, X and Y by quantitative fluorescence-PCR combined with short tandem repeat and fluorescence-labeled homologous gene quantitative‑PCR using 4-color fluorescently labeled universal primers". Molecular Medicine Reports 8, no. 5 (2013): 1601-1605. https://doi.org/10.3892/mmr.2013.1678
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