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Article

7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice

  • Authors:
    • Jian-Shi Du
    • Qing Zhao
    • Ying-Li Zhang
    • Yu Wang
    • Ming Ma
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 1525-1530
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/mmr.2013.1682
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Abstract

Nuclear factor (NF)-κB expression occurs during sciatic injury. In addition, 7,8-dihydroxycoumarin exhibits a neurotrophic effect on peripheral nerve regeneration. To investigate the effects of 7,8-dihydroxycoumarin on the expression levels of NF-κB in L4-6 spinal cord segments of the injured sciatic nerve in mice and on the functional recovery and regeneration following nerve injury, a total of 160 healthy adult male BALB/c mice underwent unilateral sciatic nerve interruption and anastomosis. The mice were separated into groups and subsequently treated with physiological saline (control) or high, medium or low doses of 7,8-dihydroxycoumarin. NF-κB levels were detected by western blot analysis and quantitative polymerase chain reaction (qPCR), and the sciatic functional index (SFI) was measured. Neuronal apoptosis was detected by terminal‑deoxynucleotidyl transferase dUTP‑mediated nick‑end labeling (TUNEL) staining. The results revealed that NF-κB was activated in the L4-6 spinal cord connected to the injured sciatic nerve. qPCR and western-blot analysis results showed that the expression levels of NF-κB in the high- and medium-dose groups were significantly lower compared with the low-dose and control groups at 12 h, one day, three days, five days and one week (P<0.05 for each). SFI and TUNEL results demonstrated that the high- and medium-dose groups exhibited improved functional nerve regeneration and reduced apoptosis compared with the low-dose and control groups. In conclusion, 7,8-dihydroxycoumarin is capable of suppressing the immune activation of NF-κB in the neurons of the L4-6 spinal cord connected with the injured sciatic nerve, thereby reducing the focal filtration of inflammatory cells, producing the optimum environment for nerve regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Du J, Zhao Q, Zhang Y, Wang Y and Ma M: 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. Mol Med Rep 8: 1525-1530, 2013.
APA
Du, J., Zhao, Q., Zhang, Y., Wang, Y., & Ma, M. (2013). 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. Molecular Medicine Reports, 8, 1525-1530. https://doi.org/10.3892/mmr.2013.1682
MLA
Du, J., Zhao, Q., Zhang, Y., Wang, Y., Ma, M."7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice". Molecular Medicine Reports 8.5 (2013): 1525-1530.
Chicago
Du, J., Zhao, Q., Zhang, Y., Wang, Y., Ma, M."7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice". Molecular Medicine Reports 8, no. 5 (2013): 1525-1530. https://doi.org/10.3892/mmr.2013.1682
Copy and paste a formatted citation
x
Spandidos Publications style
Du J, Zhao Q, Zhang Y, Wang Y and Ma M: 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. Mol Med Rep 8: 1525-1530, 2013.
APA
Du, J., Zhao, Q., Zhang, Y., Wang, Y., & Ma, M. (2013). 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice. Molecular Medicine Reports, 8, 1525-1530. https://doi.org/10.3892/mmr.2013.1682
MLA
Du, J., Zhao, Q., Zhang, Y., Wang, Y., Ma, M."7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice". Molecular Medicine Reports 8.5 (2013): 1525-1530.
Chicago
Du, J., Zhao, Q., Zhang, Y., Wang, Y., Ma, M."7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-κB expression in mice". Molecular Medicine Reports 8, no. 5 (2013): 1525-1530. https://doi.org/10.3892/mmr.2013.1682
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