Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November 2013 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November 2013 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells

  • Authors:
    • Xiaojing Sun
    • Zhiming Wang
    • Shengyong Zhai
    • Yingwen Cheng
    • Jie Liu
    • Binbin Liu
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Carcinogenesis and Cancer Invasion, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Ministry of Education, Xuhui, Shanghai 200032, P.R. China, Department of Surgical Oncology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Chemistry, Duke University, Durham, NC 27708, USA
  • Pages: 1365-1372
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/mmr.2013.1683
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The number of studies concerning silver nanoparticles (AgNPs) has increased, due in part to their potential uses for biomedical applications. These particles have been demonstrated in the elimination of the hepatitis B virus and the inhibition of the proliferation of various cancer cells in vivo and in vitro. Thus, studies on AgNPs may lead to a more efficacious and safer therapeutic approach for chronic liver injury. Hepatic stellate cells (HSCs) are essential interstitial cells in the liver and are the predominant therapeutic target in hepatic fibrosis and liver cirrhosis; however, the intracellular effects of AgNPs on HSCs remain to be elucidated. The aim of the present study was to investigate the effects of AgNPs on the function and metabolism of HSCs. Various concentrations of AgNPs, with a diameter of 10 or 30‑50 nm, were incubated with HSCs. Transmission electron microscopy, flow cytometry, enzyme‑linked immunosorbent assays, and apoptosis and proliferation detection kits were used to analyze the effects of AgNPs on cell proliferation and metabolism. These studies demonstrated that AgNPs inhibited the proliferation of HSCs and induced their apoptosis in a size- and dose‑dependent manner.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

AshaRani PV, Low Kah Mun G, Hande MP and Valiyaveettil S: Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 3:279–290. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Xu X, Yang Q, Bai J, Lu T, Li Y and Jing X: Fabrication of biodegradable electrospun poly(L-lactide-co-glycolide) fibers with antimicrobial nanosilver particles. J Nanosci Nanotechno. 8:5066–5070. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Lu L, Sun RW, Chen R, Hui CK, Ho CM, Luk JM, Lau GK and Che CM: Silver nanoparticles inhibit hepatitis B virus replication. Antivir Ther. 13:253–262. 2008.PubMed/NCBI

4 

Schroeder S, Heller DA, Winslow MM, et al: Treating metastatic cancer with nanotechnology. Nat Rev Cancer. 12:39–50. 2011. View Article : Google Scholar

5 

Weiskirchen R and Gressner AM: Isolation and culture of hepatic stellate cells. Methods Mol Med. 117:99–113. 2005.PubMed/NCBI

6 

Lara HH, Ayala-Nuñez NV, Ixtepan-Turrent L and Rodriguez-Padilla C: Mode of antiviral action of silver nanoparticles against HIV-1. J Nanobiotechnology. 8:1–10. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Gopinath P, Gogoi SK, Chattopadhyay A and Ghosh SS: Implications of silver nanoparticle induced cell apoptosis for in vitro gene therapy. Nanotechnology. 19:104–113. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Yuan Y, Liu C, Qian J, Wang J and Zhang Y: Size-mediated cytotoxicity and apoptosis of hydroxyapatite nanoparticles in human hepatoma HepG2 cells. Biomaterials. 31:730–740. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Abdelhalim MA and Jarrar BM: Gold nanoparticles induced cloudy swelling to hydropic degeneration, cytoplasmic hyaline vacuolation, polymorphism, binucleation, karyopyknosis, karyolysis, karyorrhexis and necrosis in the liver. Lipids Health Dis. 10:1662011. View Article : Google Scholar

10 

Li Y, Tian X, Lu Z, Yang C, Yang G, Zhou X, Yao H, Zhu Z, Xi Z and Yang X: Mechanism for alpha-MnO2 nanowire-induced cytotoxicity in Hela cells. J Nanosci Nanotechnol. 10:397–404. 2010.PubMed/NCBI

11 

Zamzami N and Kroemer G: Condensed matter in cell death. Nature. 401:127–128. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Urne AG and Vaux DL: Molecular and clinical aspects of apoptosis. Pharmacol Ther. 72:37–50. 1996. View Article : Google Scholar

13 

Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA and Syed AF: Metal-based nanoparticles and their toxicity assessment. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2:544–568. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Green DR and Reed JC: Mitochondria and apoptosis. Science. 281:1309–1312. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Zamzami N, Marchetti P, Castedo M, Zanin C, Vayssière JL, Petit PX and Kroemer G: Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo. J Exp Med. 181:1661–1672. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Piao MJ, Kang KA, Lee IK, Kim HS, Kim S, Choi JY, Choi J and Hyun JW: Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett. 201:92–100. 2011. View Article : Google Scholar

17 

Kroemer G, Zamzami N and Susin SA: Mitochondrial control of apoptosis. Immunol Today. 18:44–51. 1997. View Article : Google Scholar

18 

Chung TW, Kim JR, Suh JI, Lee YC, Chang YC, Chung TH and Kim CH: Correlation between plasma levels of matrix metalloproteinase (MMP)-9/MMP-2 ratio and alpha-fetoproteins in chronic hepatitis carrying hepatitis B virus. J Gastroen Hepatol. 19:565–571. 2004. View Article : Google Scholar

19 

Lichtinghagen R, Huegel O, Seifert T, et al: Expression of matrix metalloproteinase-2 and −9 and their inhibitors in peripheral blood cells of patients with chronic hepatitis C. Clin Chem. 46:183–192. 2000.

20 

Chen JS, Wang Q, Fu XH, Huang XH, Chen XL, Cao LQ, Chen LZ, Tan HX, Li W, Bi J and Zhang LJ: Involvement of PI3K/PTEN/AKT/mTOR pathway in invasion and metastasis in hepatocellular carcinoma: Association with MMP-9. Hepatol Res. 39:177–186. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sun X, Wang Z, Zhai S, Cheng Y, Liu J and Liu B: In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells. Mol Med Rep 8: 1365-1372, 2013.
APA
Sun, X., Wang, Z., Zhai, S., Cheng, Y., Liu, J., & Liu, B. (2013). In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells. Molecular Medicine Reports, 8, 1365-1372. https://doi.org/10.3892/mmr.2013.1683
MLA
Sun, X., Wang, Z., Zhai, S., Cheng, Y., Liu, J., Liu, B."In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells". Molecular Medicine Reports 8.5 (2013): 1365-1372.
Chicago
Sun, X., Wang, Z., Zhai, S., Cheng, Y., Liu, J., Liu, B."In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells". Molecular Medicine Reports 8, no. 5 (2013): 1365-1372. https://doi.org/10.3892/mmr.2013.1683
Copy and paste a formatted citation
x
Spandidos Publications style
Sun X, Wang Z, Zhai S, Cheng Y, Liu J and Liu B: In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells. Mol Med Rep 8: 1365-1372, 2013.
APA
Sun, X., Wang, Z., Zhai, S., Cheng, Y., Liu, J., & Liu, B. (2013). In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells. Molecular Medicine Reports, 8, 1365-1372. https://doi.org/10.3892/mmr.2013.1683
MLA
Sun, X., Wang, Z., Zhai, S., Cheng, Y., Liu, J., Liu, B."In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells". Molecular Medicine Reports 8.5 (2013): 1365-1372.
Chicago
Sun, X., Wang, Z., Zhai, S., Cheng, Y., Liu, J., Liu, B."In vitro cytotoxicity of silver nanoparticles in primary rat hepatic stellate cells". Molecular Medicine Reports 8, no. 5 (2013): 1365-1372. https://doi.org/10.3892/mmr.2013.1683
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team