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Article

Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation

  • Authors:
    • Guo-Jun Xu
    • Tian-Yi Gan
    • Bao-Peng Tang
    • Zu-Heng Chen
    • Ailiman Mahemuti
    • Tao Jiang
    • Jian-Guo Song
    • Xia Guo
    • Yao-Dong Li
    • Xian-Hui Zhou
    • Yu Zhang
    • Jin-Xin Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Animal Experiment, First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Laboratory of Electrophysiology, First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Molecular Biology, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China
  • Pages: 1343-1352
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/mmr.2013.1684
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Abstract

The aim of this study was to investigate the correlation between the change in the expression of atrial calpains and electrical, molecular and structural remodeling during aging and atrial fibrillation (AF). Adult and aged canines in sinus rhythm (SR) and with persistent AF (induced by rapid atrial pacing) were investigated. A whole-cell patch clamp was used to measure the L‑type Ca2+ current (ICa-L) in cells in the left atrium. The mRNA and protein expression of the L‑type calcium channel alc subunit (LVDCCa1c) and calpains were measured by quantitative (q)PCR and western blot analysis. Histopathological and ultrastructural changes were analyzed via light and electron microscopy. The quantity of apoptotic myocytes was determined by a terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling (TUNEL) assay. In SR groups, atrial cells of the aged canines exhibited a longer action potential (AP) duration to 90% repolarization (APD90), lower AP plateau potential and peak ICa-L current densities (P<0.05). In the adult and aged groups, AF led to a higher maximum diastolic potential, an increase in AP amplitude and decreases in APD90, AP plateau potential and peak ICa-L densities (P<0.05). Compared with the control group, the mRNA and protein expression levels of LVDCCa1c were decreased in the aged groups; however, the mRNA and protein expression of calpain 1 was increased in the adult and the aged groups with AF (P<0.05). Samples of atrial tissue exhibited abnormal histopathological and ultrastructural changes, such as accelerated fibrosis and apoptosis with aging and in AF. Age‑related alterations in atrial tissues were attributed to the increased expression of calpain 1. The general pathophysiological alterations in normal aged atria may therefore produce a substrate that is conducive to AF.
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Copy and paste a formatted citation
Spandidos Publications style
Xu G, Gan T, Tang B, Chen Z, Mahemuti A, Jiang T, Song J, Guo X, Li Y, Zhou X, Zhou X, et al: Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation. Mol Med Rep 8: 1343-1352, 2013.
APA
Xu, G., Gan, T., Tang, B., Chen, Z., Mahemuti, A., Jiang, T. ... Li, J. (2013). Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation. Molecular Medicine Reports, 8, 1343-1352. https://doi.org/10.3892/mmr.2013.1684
MLA
Xu, G., Gan, T., Tang, B., Chen, Z., Mahemuti, A., Jiang, T., Song, J., Guo, X., Li, Y., Zhou, X., Zhang, Y., Li, J."Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation". Molecular Medicine Reports 8.5 (2013): 1343-1352.
Chicago
Xu, G., Gan, T., Tang, B., Chen, Z., Mahemuti, A., Jiang, T., Song, J., Guo, X., Li, Y., Zhou, X., Zhang, Y., Li, J."Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation". Molecular Medicine Reports 8, no. 5 (2013): 1343-1352. https://doi.org/10.3892/mmr.2013.1684
Copy and paste a formatted citation
x
Spandidos Publications style
Xu G, Gan T, Tang B, Chen Z, Mahemuti A, Jiang T, Song J, Guo X, Li Y, Zhou X, Zhou X, et al: Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation. Mol Med Rep 8: 1343-1352, 2013.
APA
Xu, G., Gan, T., Tang, B., Chen, Z., Mahemuti, A., Jiang, T. ... Li, J. (2013). Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation. Molecular Medicine Reports, 8, 1343-1352. https://doi.org/10.3892/mmr.2013.1684
MLA
Xu, G., Gan, T., Tang, B., Chen, Z., Mahemuti, A., Jiang, T., Song, J., Guo, X., Li, Y., Zhou, X., Zhang, Y., Li, J."Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation". Molecular Medicine Reports 8.5 (2013): 1343-1352.
Chicago
Xu, G., Gan, T., Tang, B., Chen, Z., Mahemuti, A., Jiang, T., Song, J., Guo, X., Li, Y., Zhou, X., Zhang, Y., Li, J."Alterations in the expression of atrial calpains in electrical and structural remodeling during aging and atrial fibrillation". Molecular Medicine Reports 8, no. 5 (2013): 1343-1352. https://doi.org/10.3892/mmr.2013.1684
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