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Article

Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells

  • Authors:
    • Bin Liu
    • Jia Wang
    • Lan Cheng
    • Jingping Liang
  • View Affiliations / Copyright

    Affiliations: Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China, Department of Periodontology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China
  • Pages: 1594-1600
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    Published online on: September 17, 2013
       https://doi.org/10.3892/mmr.2013.1685
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Abstract

An increasing number of studies have shown a correlation between Porphyromonas gingivalis (P. gingivalis) infection and atherosclerosis. A recent study demonstrated that the expression of vascular cell adhesion molecule‑1 (VCAM‑1) was induced by P. gingivalis lipopolysaccharide (LPS) in human aortic endothelial cells (HAECs). The activation of p38 mitogen‑activated protein kinase (p38 MAPK) was at least partially involved in this process. Those results suggested the potential involvement of P. gingivalis LPS in the pathogenesis of atherosclerosis. However, the mechanism involved in P. gingivalis LPS‑induced VCAM‑1 production has not yet been elucidated. The present study examined the role of the c‑Jun N‑terminal kinase (JNK) and nuclear factor‑κB (NF‑κB) cell signaling pathways in P. gingivalis LPS‑induced VCAM‑1 expression in HAECs. Western blotting was used to investigate the activation of JNK and NF‑κB pathways in HAECs exposed to P. gingivalis LPS. Following this, specific pharmacological inhibitors were introduced and the protein production of VCAM‑1 was studied. The results showed that the JNK and NF‑κB pathways in HAECs were capable of being activated by P. gingivalis LPS. The inhibition of NF‑κB by SN50 significantly attenuated P. gingivalis LPS‑induced VCAM‑1 expression, while the inhibition of JNK by SP600125 enhanced VCAM‑1 expression in P. gingivalis LPS‑treated HAECs. Therefore, the results indicated that NF‑κB was essential for the P. gingivalis LPS‑induced VCAM‑1 expression in HAECs and that JNK may be a suppressor of VCAM‑1 expression in HAECs.
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Copy and paste a formatted citation
Spandidos Publications style
Liu B, Wang J, Cheng L and Liang J: Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells. Mol Med Rep 8: 1594-1600, 2013.
APA
Liu, B., Wang, J., Cheng, L., & Liang, J. (2013). Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells. Molecular Medicine Reports, 8, 1594-1600. https://doi.org/10.3892/mmr.2013.1685
MLA
Liu, B., Wang, J., Cheng, L., Liang, J."Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells". Molecular Medicine Reports 8.5 (2013): 1594-1600.
Chicago
Liu, B., Wang, J., Cheng, L., Liang, J."Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells". Molecular Medicine Reports 8, no. 5 (2013): 1594-1600. https://doi.org/10.3892/mmr.2013.1685
Copy and paste a formatted citation
x
Spandidos Publications style
Liu B, Wang J, Cheng L and Liang J: Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells. Mol Med Rep 8: 1594-1600, 2013.
APA
Liu, B., Wang, J., Cheng, L., & Liang, J. (2013). Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells. Molecular Medicine Reports, 8, 1594-1600. https://doi.org/10.3892/mmr.2013.1685
MLA
Liu, B., Wang, J., Cheng, L., Liang, J."Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells". Molecular Medicine Reports 8.5 (2013): 1594-1600.
Chicago
Liu, B., Wang, J., Cheng, L., Liang, J."Role of JNK and NF‑κB pathways in Porphyromonas gingivalis LPS‑induced vascular cell adhesion molecule‑1 expression in human aortic endothelial cells". Molecular Medicine Reports 8, no. 5 (2013): 1594-1600. https://doi.org/10.3892/mmr.2013.1685
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