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Article

Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG

  • Authors:
    • Junwei Zhao
    • Zhanqiang Sun
    • Hao Pei
    • Juan Ye
    • Cuicui Chen
    • Buka Samten
    • Shulin Zhang
    • Xiaokui Guo
  • View Affiliations / Copyright

    Affiliations: Department of Medical Microbiology and Parasitology, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, P.R. China, Clinical Laboratory, Wuxi No. 5 People's Hospital, Wuxi, Jiangsu 214005, P.R. China, Center for Pulmonary and Infectious Disease Control, The University of Texas Health Science Center, Tyler, TX 75708, USA
  • Pages: 1587-1593
    |
    Published online on: September 17, 2013
       https://doi.org/10.3892/mmr.2013.1687
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Abstract

Tuberculosis (TB) remains a global infectious disease. To investigate the value of a novel Mycobacterium tuberculosis (M. tuberculosis) region of difference 5 (RD5)‑encoded antigen, Rv3117, in the development of effective immuno­diagnostics and vaccines against TB, the immune responses to the antigen were examined in human subjects, as well as in C57BL/6 mice. The results showed that Rv3117 was able to evoke specific humoral and cellular immune responses. Consistent with the results from the RD1‑encoded antigens, culture filtrate protein 10 kDa (CFP‑10) and early secreted antigenic target 6 kDa (ESAT‑6), the immunoglobulin G (IgG), IgM and IgA antibody responses to Rv3117 were able to statistically distinguish between the 65 patients with active pulmonary TB and the 59 healthy controls (P<0.01, respectively). In addition, higher levels of Rv3117‑specific interferon‑γ (IFN‑γ) were observed in immunized C57BL/6 mice than in the negative control mice (P<0.05). Furthermore, high titers of total IgG, IgG1 and IgG2a antibodies were present in the sera from immunized mice, even six weeks subsequent to the immunization. In conclusion, the present results suggested that Rv3117 may be used as a candidate for the development of TB immunodiagnostics and vaccine design.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Sun Z, Pei H, Ye J, Chen C, Samten B, Zhang S and Guo X: Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG. Mol Med Rep 8: 1587-1593, 2013.
APA
Zhao, J., Sun, Z., Pei, H., Ye, J., Chen, C., Samten, B. ... Guo, X. (2013). Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG. Molecular Medicine Reports, 8, 1587-1593. https://doi.org/10.3892/mmr.2013.1687
MLA
Zhao, J., Sun, Z., Pei, H., Ye, J., Chen, C., Samten, B., Zhang, S., Guo, X."Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG". Molecular Medicine Reports 8.5 (2013): 1587-1593.
Chicago
Zhao, J., Sun, Z., Pei, H., Ye, J., Chen, C., Samten, B., Zhang, S., Guo, X."Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG". Molecular Medicine Reports 8, no. 5 (2013): 1587-1593. https://doi.org/10.3892/mmr.2013.1687
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Sun Z, Pei H, Ye J, Chen C, Samten B, Zhang S and Guo X: Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG. Mol Med Rep 8: 1587-1593, 2013.
APA
Zhao, J., Sun, Z., Pei, H., Ye, J., Chen, C., Samten, B. ... Guo, X. (2013). Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG. Molecular Medicine Reports, 8, 1587-1593. https://doi.org/10.3892/mmr.2013.1687
MLA
Zhao, J., Sun, Z., Pei, H., Ye, J., Chen, C., Samten, B., Zhang, S., Guo, X."Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG". Molecular Medicine Reports 8.5 (2013): 1587-1593.
Chicago
Zhao, J., Sun, Z., Pei, H., Ye, J., Chen, C., Samten, B., Zhang, S., Guo, X."Immunological evaluation of a novel Mycobacterium tuberculosis antigen, Rv3117, absent in Mycobacterium bovis BCG". Molecular Medicine Reports 8, no. 5 (2013): 1587-1593. https://doi.org/10.3892/mmr.2013.1687
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