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Article

Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn

  • Authors:
    • Shen Wang
    • Han‑Chun Chen
    • Cai‑Bin Fan
    • Duan‑Gai Wen
    • Jian‑Quan Hou
    • Jun Ouyang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Fourth People's Hospital of Changzhou, Changzhou, Jiangsu 213032, P.R. China, Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, Suzhou, Jiangsu 215007, P.R. China, Department of Urology, Shaoxing County Center Hospital, Shaoxing, Zhejiang 312030, P.R. China, Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
  • Pages: 1519-1524
    |
    Published online on: September 17, 2013
       https://doi.org/10.3892/mmr.2013.1689
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Abstract

Previous studies have demonstrated that recipient‑derived immature dendritic cells transfected by recombinant adenovirus‑mediated IKK2dn (Adv‑IKK2dn) and loaded with donor splenocyte lysate generate CD4+CD25‑ T cells (Adv‑IKK2dn‑CD4+CD25‑ T cells). These cells may inhibit T cell responses in vitro. In the present study, Lewis (LW) rats were administered with an intravenous injection of naive CD4+ T cells, empty adenovirus (Adv‑0)‑dendritic cell‑generated CD4+CD25‑ T cells (Adv‑0‑CD4+CD25‑ T cells), Adv‑IKK2dn‑CD4+CD25‑ T cells or an equal volume of normal saline, seven days prior to transplantation. The potency and the mechanism of action of Adv‑IKK2dn‑CD4+CD25‑ T cells was analyzed, as well as an investigation of their tolerogenic properties in vivo. Administration of Adv‑IKK2dn‑CD4+CD25‑ T cells in vivo to LW rats was observed to markedly prolong the survival of a kidney allograft from Brown Norway rats. Furthermore, the Adv‑IKK2dn‑CD4+CD25‑ T cell‑treated group exhibited significantly reduced levels of interleukin (Il)‑2 and interferon‑γ production and increased Il‑10 and transforming growth factor‑β (TGF‑β) secretion. The serum creatinine levels remained at low levels in the Adv‑IKK2dn‑CD4+CD25‑ T cell‑treated group. Their ability to induce allogeneic T cell proliferation was markedly reduced compared with the other groups. These observations indicated that Adv‑IKK2dn‑CD4+CD25‑ T cells induce prolongation of kidney allograft survival in vivo, which is hypothesized to be due to the high expression levels of Il‑10 and TGF‑β.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Chen HC, Fan CB, Wen DG, Hou JQ and Ouyang J: Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn. Mol Med Rep 8: 1519-1524, 2013.
APA
Wang, S., Chen, H., Fan, C., Wen, D., Hou, J., & Ouyang, J. (2013). Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn. Molecular Medicine Reports, 8, 1519-1524. https://doi.org/10.3892/mmr.2013.1689
MLA
Wang, S., Chen, H., Fan, C., Wen, D., Hou, J., Ouyang, J."Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn". Molecular Medicine Reports 8.5 (2013): 1519-1524.
Chicago
Wang, S., Chen, H., Fan, C., Wen, D., Hou, J., Ouyang, J."Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn". Molecular Medicine Reports 8, no. 5 (2013): 1519-1524. https://doi.org/10.3892/mmr.2013.1689
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Chen HC, Fan CB, Wen DG, Hou JQ and Ouyang J: Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn. Mol Med Rep 8: 1519-1524, 2013.
APA
Wang, S., Chen, H., Fan, C., Wen, D., Hou, J., & Ouyang, J. (2013). Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn. Molecular Medicine Reports, 8, 1519-1524. https://doi.org/10.3892/mmr.2013.1689
MLA
Wang, S., Chen, H., Fan, C., Wen, D., Hou, J., Ouyang, J."Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn". Molecular Medicine Reports 8.5 (2013): 1519-1524.
Chicago
Wang, S., Chen, H., Fan, C., Wen, D., Hou, J., Ouyang, J."Prolongation of rat renal allograft survival by CD4+CD25‑ T cells induced by recipient dendritic cells transfected with IKK2dn". Molecular Medicine Reports 8, no. 5 (2013): 1519-1524. https://doi.org/10.3892/mmr.2013.1689
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