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Article

Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy

  • Authors:
    • Lilin Wang
    • Wei Tang
    • Shirong Yan
    • Long Zhou
    • Tao Shen
    • Xiuqing  Huang
    • Lin Dou
    • Mo Wang
    • Songlin Yu
    • Jian Li
  • View Affiliations / Copyright

    Affiliations: Center for Gene Diagnosis, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China, Life Science Research Institute of Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Life Science Research Institute of Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Medicine, Shenzhen Family Planning Service Center, Shenzhen, Guangdong 518028, P.R. China, The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics and Beijing Hospital, Ministry of Health, Dongdan, Beijing 100730, P.R. China
  • Pages: 1472-1478
    |
    Published online on: September 18, 2013
       https://doi.org/10.3892/mmr.2013.1691
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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs that are important in the pathogenesis of multiple diseases and, therefore, may represent a novel class of targets for therapeutic intervention. However, like the majority of oligonucleotide‑based strategies, there are obstacles to their clinical application, including poor cellular uptake due to the low permeability of the cell membrane to negatively charged molecules. MPG is a 27‑residue peptide vector which contains a hydrophobic domain derived from the fusion sequence of HIV-1 gp41 and a hydrophilic domain derived from the nuclear localization sequence of SV40 T-antigen. MPG is one of the most promising tools for the non‑invasive cellular import of oligonucleotides and analogs. In the present study, a non‑covalent peptide‑based strategy was used for the efficient delivery of the miRNA‑122 (miR‑122) mimic and inhibitor into mouse liver cell lines, mouse primary hepatocytes and C. elegans, without any associated cytotoxicity. Moreover, high‑performance liquid chromatography analysis determined that MPG and MPGΔNLS delivered the miR‑122 mimic and inhibitor into mouse liver cells and effectively regulated cholesterol levels. The results demonstrated that MPG family members may be used for the efficient delivery of miR‑122 to regulate cholesterol metabolism, and that this cell‑penetrating peptide‑based technology may be beneficial for further biological applications of RNA therapeutics in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Tang W, Yan S, Zhou L, Shen T, Huang X, Dou L, Wang M, Yu S, Li J, Li J, et al: Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy. Mol Med Rep 8: 1472-1478, 2013.
APA
Wang, L., Tang, W., Yan, S., Zhou, L., Shen, T., Huang, X. ... Li, J. (2013). Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy. Molecular Medicine Reports, 8, 1472-1478. https://doi.org/10.3892/mmr.2013.1691
MLA
Wang, L., Tang, W., Yan, S., Zhou, L., Shen, T., Huang, X., Dou, L., Wang, M., Yu, S., Li, J."Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy". Molecular Medicine Reports 8.5 (2013): 1472-1478.
Chicago
Wang, L., Tang, W., Yan, S., Zhou, L., Shen, T., Huang, X., Dou, L., Wang, M., Yu, S., Li, J."Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy". Molecular Medicine Reports 8, no. 5 (2013): 1472-1478. https://doi.org/10.3892/mmr.2013.1691
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Tang W, Yan S, Zhou L, Shen T, Huang X, Dou L, Wang M, Yu S, Li J, Li J, et al: Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy. Mol Med Rep 8: 1472-1478, 2013.
APA
Wang, L., Tang, W., Yan, S., Zhou, L., Shen, T., Huang, X. ... Li, J. (2013). Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy. Molecular Medicine Reports, 8, 1472-1478. https://doi.org/10.3892/mmr.2013.1691
MLA
Wang, L., Tang, W., Yan, S., Zhou, L., Shen, T., Huang, X., Dou, L., Wang, M., Yu, S., Li, J."Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy". Molecular Medicine Reports 8.5 (2013): 1472-1478.
Chicago
Wang, L., Tang, W., Yan, S., Zhou, L., Shen, T., Huang, X., Dou, L., Wang, M., Yu, S., Li, J."Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based strategy". Molecular Medicine Reports 8, no. 5 (2013): 1472-1478. https://doi.org/10.3892/mmr.2013.1691
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