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November 2013 Volume 8 Issue 5

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Article

Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro

  • Authors:
    • Hua Nan
    • Jiacheng Huang
    • Hongmian Li
    • Qiong Li
    • Dalie Liu
  • View Affiliations / Copyright

    Affiliations: Plastic Surgery Institute, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Plastic Surgery Institute, Zhongshan Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510275, P.R. China
  • Pages: 1446-1452
    |
    Published online on: September 18, 2013
       https://doi.org/10.3892/mmr.2013.1694
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Abstract

The current study aimed to investigate adipose tissue‑derived stem cells (ADSCs) in vivo by multimodality imaging following implantation for cellular therapy. The biological characteristics of ADSCs co‑labeled with Molday ION Rhodamine B™ (MIRB) and green fluorescent protein (GFP) were studied in vitro. Following rat ADSC isolation and culture, a combined labeling strategy for ADSCs based on genetic modification of the reporter gene GFP with lentiviral vector expression enhancement and physical MIRB labeling was performed. Cell viability, proliferation, membrane‑bound antigens and multiple differentiation ability were compared between the labeled and unlabeled ADSCs. The ADSCs were successfully labeled with GFP and MIRB, showing various fluorescent colors for marker identification. The fluorescence emitted by the GFP protein was sustained and exhibited stable expression, while MIRB fluorescence decreased with time. Compared with the unlabeled ADSCs, no significant differences were detected in cell viability, proliferation, membrane‑bound antigens and multiple differentiation ability in the co‑labeled samples (P>0.05). No significant effects on the biophysical properties of ADSCs were observed following co‑labeling with lentiviral vectors encoding the gene for emerald green fluorescent protein and MIRB. The ADSCs were able to be efficiently tracked in vitro and in vivo by multimodality imaging thus, the co‑labeling approach provides a novel strategy for therapeutic gene studies.
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Copy and paste a formatted citation
Spandidos Publications style
Nan H, Huang J, Li H, Li Q and Liu D: Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro. Mol Med Rep 8: 1446-1452, 2013.
APA
Nan, H., Huang, J., Li, H., Li, Q., & Liu, D. (2013). Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro. Molecular Medicine Reports, 8, 1446-1452. https://doi.org/10.3892/mmr.2013.1694
MLA
Nan, H., Huang, J., Li, H., Li, Q., Liu, D."Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro". Molecular Medicine Reports 8.5 (2013): 1446-1452.
Chicago
Nan, H., Huang, J., Li, H., Li, Q., Liu, D."Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro". Molecular Medicine Reports 8, no. 5 (2013): 1446-1452. https://doi.org/10.3892/mmr.2013.1694
Copy and paste a formatted citation
x
Spandidos Publications style
Nan H, Huang J, Li H, Li Q and Liu D: Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro. Mol Med Rep 8: 1446-1452, 2013.
APA
Nan, H., Huang, J., Li, H., Li, Q., & Liu, D. (2013). Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro. Molecular Medicine Reports, 8, 1446-1452. https://doi.org/10.3892/mmr.2013.1694
MLA
Nan, H., Huang, J., Li, H., Li, Q., Liu, D."Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro". Molecular Medicine Reports 8.5 (2013): 1446-1452.
Chicago
Nan, H., Huang, J., Li, H., Li, Q., Liu, D."Assessment of biological characteristics of adipose tissue‑derived stem cells co‑labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro". Molecular Medicine Reports 8, no. 5 (2013): 1446-1452. https://doi.org/10.3892/mmr.2013.1694
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