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Article

Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy

  • Authors:
    • Yanping Wang
    • Qiong Wei
    • Qiang Liu
    • Zheng Li
    • Li Zhou
    • Fang Zou
    • Yang Yuan
    • Zilin Sun
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Zhongda Hospital, Institute of Diabetes, Medical School, Southeast University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 1817-1823
    |
    Published online on: September 25, 2013
       https://doi.org/10.3892/mmr.2013.1703
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Abstract

An increasing number of studies suggest that the activation of innate immunity with the development of a chronic low‑grade inflammatory response is a factor in the pathogenesis of diabetic nephropathy (DN). Advanced glycation end products (AGEs), chemokines and matrix metalloproteinases (MMPs) are known to be important in inflammatory reactions in DN. In the present study, the inter-regulation of MMP2 and fractalkine was observed between monocytes (U937) and human renal mesangial cells (HRMCs) and its potential pathophysiological role in DN. The expression of fractalkine and MMP2 was analyzed by RT-PCR, western blot analysis and enzyme‑linked immunosorbent assay. The chemotaxis and adhesiveness of HRMCs to U937 cells was detected with a transwell system, co‑culture and fluorescent staining, respectively. The results showed a decreased expression of MMP2 and an increased expression of fractalkine by AGEs in HRMCs. Fractalkine downregulated the mRNA expression and activity of MMP2, and the reduced MMP2 activity was reversed with an anti‑fractalkine antibody. Conversely, MMP2 upregulated fractalkine mRNA and protein expression in HRMCs, which led to an increase in chemotaxis and a decrease in monocytic adhesion to HRMCs. In conclusion, these observations suggest a crosstalk between monocytes and HRMCs via the interaction of MMP2 and fractalkine, which may represent a therapeutic target to impede the inflammatory process associated with DN.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y and Sun Z: Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy. Mol Med Rep 8: 1817-1823, 2013.
APA
Wang, Y., Wei, Q., Liu, Q., Li, Z., Zhou, L., Zou, F. ... Sun, Z. (2013). Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy. Molecular Medicine Reports, 8, 1817-1823. https://doi.org/10.3892/mmr.2013.1703
MLA
Wang, Y., Wei, Q., Liu, Q., Li, Z., Zhou, L., Zou, F., Yuan, Y., Sun, Z."Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy". Molecular Medicine Reports 8.6 (2013): 1817-1823.
Chicago
Wang, Y., Wei, Q., Liu, Q., Li, Z., Zhou, L., Zou, F., Yuan, Y., Sun, Z."Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy". Molecular Medicine Reports 8, no. 6 (2013): 1817-1823. https://doi.org/10.3892/mmr.2013.1703
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y and Sun Z: Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy. Mol Med Rep 8: 1817-1823, 2013.
APA
Wang, Y., Wei, Q., Liu, Q., Li, Z., Zhou, L., Zou, F. ... Sun, Z. (2013). Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy. Molecular Medicine Reports, 8, 1817-1823. https://doi.org/10.3892/mmr.2013.1703
MLA
Wang, Y., Wei, Q., Liu, Q., Li, Z., Zhou, L., Zou, F., Yuan, Y., Sun, Z."Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy". Molecular Medicine Reports 8.6 (2013): 1817-1823.
Chicago
Wang, Y., Wei, Q., Liu, Q., Li, Z., Zhou, L., Zou, F., Yuan, Y., Sun, Z."Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy". Molecular Medicine Reports 8, no. 6 (2013): 1817-1823. https://doi.org/10.3892/mmr.2013.1703
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