Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December 2013 Volume 8 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December 2013 Volume 8 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

A novel four-step system for screening angiogenesis inhibitors

  • Authors:
    • Qin Zhou
    • Cui-Ling Qi
    • Yan Li
    • Xiao-Dong He
    • Jiang-Chao Li
    • Qian-Qian Zhang
    • Lan Tian
    • Ming Zhang
    • Zhe Han
    • Huiping Wang
    • Xuesong Yang
    • Li-Jing Wang
  • View Affiliations / Copyright

    Affiliations: Vascular Biology Research Institute, Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Division of Histology and Embryology, Medical College, Jinan University, Guangzhou, Guangdong 510632, P.R. China
  • Pages: 1734-1740
    |
    Published online on: September 26, 2013
       https://doi.org/10.3892/mmr.2013.1704
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Angiogenesis exhibits a significant effect on tumor progression. Inhibiting angiogenesis may provide significant advantages over currently available therapeutics for cancer therapies thus, the development of a system of screening angiogenesis is essential. In the present study, a novel available system of screening angiogenesis inhibitors by four steps was developed. The chorioallantoic membrane (CAM), yolk sac membrane and early chick embryo blood island assay were initially performed to obtain possible antitumor compounds. The MMTV‑PyMT transgenic breast cancer mouse model was used for final screening and to confirm potential antitumor effects. Four angiogenesis inhibitors were isolated from 480 compounds, which were obtained from ICCB known bioactives library, by a combination of the CAM, yolk sac membrane and early chick embryo blood island assay. The MMTV‑PyMT mouse was treated with one of four agents and it was demonstrated that the tumor volume was significantly inhibited. These results demonstrate that the four‑step screening system is feasible.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Risau W, Sariola H, Zerwes HG, et al: Vasculogenesis and angiogenesis in embryonic-stem-cell-derived embryoid bodies. Development. 102:471–478. 1988.PubMed/NCBI

2 

Weidner N, Folkman J, Pozza F, et al: Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst. 84:1875–1887. 1992. View Article : Google Scholar : PubMed/NCBI

3 

Mendel DB, Laird AD, Xin X, et al: In vivo antitumor activity of SU11248, a novel tyrosine kinase inhibitor targeting vascular endothelial growth factor and platelet-derived growth factor receptors: determination of a pharmacokinetic/pharmacodynamic relationship. Clin Cancer Res. 9:327–337. 2003.

4 

Toppmeyer DL, Gounder M, Much J, et al: A phase I and pharmacologic study of the combination of marimastat and paclitaxel in patients with advanced malignancy. Med Sci Monit. 9:PI99–PI104. 2003.PubMed/NCBI

5 

Herbst RS: Targeted therapy using novel agents in the treatment of non-small-cell lung cancer. Clin Lung Cancer. 3(Suppl 1): S30–S38. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Ribatti D, Nico B, Vacca A, Roncali L, Burri PH and Djonov V: Chorioallantoic membrane capillary bed: a useful target for studying angiogenesis and anti-angiogenesis in vivo. Anat Rec. 264:317–324. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Ausprunk DH, Knighton DR and Folkman J: Differentiation of vascular endothelium in the chick chorioallantois: a structural and autoradiographic study. Dev Biol. 38:237–248. 1974. View Article : Google Scholar : PubMed/NCBI

8 

Ribatti D, Vacca A, Roncali L and Dammacco F: The chick embryo chorioallantoic membrane as a model for in vivo research on angiogenesis. Int J Dev Biol. 40:1189–1197. 1996.PubMed/NCBI

9 

Wilting J, Christ B and Bokeloh M: A modified chorioallantoic membrane (CAM) assay for qualitative and quantitative study of growth factors. Studies on the effects of carriers, PBS, angiogenin, and bFGF. Anat Embryol (Berl). 183:259–271. 1991. View Article : Google Scholar : PubMed/NCBI

10 

Olivo M, Bhardwaj R, Schulze-Osthoff K, Sorg C, Jacob HJ and Flamme I: A comparative study on the effects of tumor necrosis factor-alpha (TNF-alpha), human angiogenic factor (h-AF) and basic fibroblast growth factor (bFGF) on the chorioallantoic membrane of the chick embryo. Anat Rec. 234:105–115. 1992. View Article : Google Scholar

11 

Leibovich SJ, Polverini PJ, Shepard HM, Wiseman DM, Shively V and Nuseir N: Macrophage-induced angiogenesis is mediated by tumour necrosis factor-alpha. Nature. 329:630–632. 1987. View Article : Google Scholar : PubMed/NCBI

12 

Leene W, Duyzings MJ and van Steeg C: Lymphoid stem cell identification in the developing thymus and bursa of Fabricius of the chick. Z Zellforsch Mikrosk Anat. 136:521–533. 1973. View Article : Google Scholar : PubMed/NCBI

13 

Jakob W, Jentzsch KD, Mauersberger B and Heder G: The chick embryo choriallantoic membrane as a bioassay for angiogenesis factors: reactions induced by carrier materials. Exp Pathol (Jena). 15:241–249. 1978.PubMed/NCBI

14 

Knighton DR, Fiegel VD and Phillips GD: The assay of angiogenesis. Prog Clin Biol Res. 365:291–299. 1991.

15 

Ribatti D, Nico B, Vacca A and Presta M: The gelatin sponge-chorioallantoic membrane assay. Nat Protoc. 1:85–91. 2006. View Article : Google Scholar : PubMed/NCBI

16 

le Noble F, Moyon D, Pardanaud L, et al: Flow regulates arterial-venous differentiation in the chick embryo yolk sac. Development. 131:361–375

17 

Takigawa M, Nishida Y, Suzuki F, Kishi J, Yamashita K and Hayakawa T: Induction of angiogenesis in chick yolk-sac membrane by polyamines and its inhibition by tissue inhibitors of metalloproteinases (TIMP and TIMP-2). Biochem Biophys Res Commun. 171:1264–1271. 1990. View Article : Google Scholar : PubMed/NCBI

18 

Plasswilm L, Höper J, Cordes N and Tannapfel A: Investigation of microvessel density after irradiation. Radiat Res. 151:454–460. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Dias PF, Siqueira JM Jr, Maraschin M, Ferreira AG, Gagliardi AR and Ribeiro-do-Valle RM: A polysaccharide isolated from the brown seaweed Sargassum stenophyllum exerts antivasculogenic effects evidenced by modified morphogenesis. Microvasc Res. 75:34–44. 2008.

20 

Dieterlen-Lievre F: On the origin of haemopoietic stem cells in the avian embryo: an experimental approach. J Embryol Exp Morphol. 33:607–619. 1975.PubMed/NCBI

21 

Sheng G: Primitive and definitive erythropoiesis in the yolk sac: a bird’s eye view. Int J Dev Biol. 54:1033–1043. 2010.PubMed/NCBI

22 

Guy CT, Cardiff RD and Muller WJ: Induction of mammary tumors by expression of polyomavirus middle T oncogene: a transgenic mouse model for metastatic disease. Mol Cell Biol. 12:954–961. 1992.PubMed/NCBI

23 

Lin EY, Jones JG, Li P, et al: Progression to malignancy in the polyoma middle T oncoprotein mouse breast cancer model provides a reliable model for human diseases. Am J Pathol. 163:2113–2126. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Chan T and Burggren W: Hypoxic incubation creates differential morphological effects during specific developmental critical windows in the embryo of the chicken (Gallus gallus). Respir Physiol Neurobiol. 145:251–263. 2005. View Article : Google Scholar

25 

Hamburger V and Hamilton HL: A series of normal stages in the development of the chick embryo. 1951. Dev Dyn. 195:231–272. 1992. View Article : Google Scholar : PubMed/NCBI

26 

Breier G, Albrecht U, Sterrer S and Risau W: Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation. Development. 114:521–532. 1992.PubMed/NCBI

27 

Ribatti D, Urbinati C, Nico B, Rusnati M, Roncali L and Presta M: Endogenous basic fibroblast growth factor is implicated in the vascularization of the chick embryo chorioallantoic membrane. Dev Biol. 170:39–49. 1995. View Article : Google Scholar : PubMed/NCBI

28 

Iurlaro M, Vacca A, Minischetti M, et al: Antiangiogenesis by cyclosporine. Exp Hematol. 26:1215–1222. 1998.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou Q, Qi C, Li Y, He X, Li J, Zhang Q, Tian L, Zhang M, Han Z, Wang H, Wang H, et al: A novel four-step system for screening angiogenesis inhibitors. Mol Med Rep 8: 1734-1740, 2013.
APA
Zhou, Q., Qi, C., Li, Y., He, X., Li, J., Zhang, Q. ... Wang, L. (2013). A novel four-step system for screening angiogenesis inhibitors. Molecular Medicine Reports, 8, 1734-1740. https://doi.org/10.3892/mmr.2013.1704
MLA
Zhou, Q., Qi, C., Li, Y., He, X., Li, J., Zhang, Q., Tian, L., Zhang, M., Han, Z., Wang, H., Yang, X., Wang, L."A novel four-step system for screening angiogenesis inhibitors". Molecular Medicine Reports 8.6 (2013): 1734-1740.
Chicago
Zhou, Q., Qi, C., Li, Y., He, X., Li, J., Zhang, Q., Tian, L., Zhang, M., Han, Z., Wang, H., Yang, X., Wang, L."A novel four-step system for screening angiogenesis inhibitors". Molecular Medicine Reports 8, no. 6 (2013): 1734-1740. https://doi.org/10.3892/mmr.2013.1704
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Q, Qi C, Li Y, He X, Li J, Zhang Q, Tian L, Zhang M, Han Z, Wang H, Wang H, et al: A novel four-step system for screening angiogenesis inhibitors. Mol Med Rep 8: 1734-1740, 2013.
APA
Zhou, Q., Qi, C., Li, Y., He, X., Li, J., Zhang, Q. ... Wang, L. (2013). A novel four-step system for screening angiogenesis inhibitors. Molecular Medicine Reports, 8, 1734-1740. https://doi.org/10.3892/mmr.2013.1704
MLA
Zhou, Q., Qi, C., Li, Y., He, X., Li, J., Zhang, Q., Tian, L., Zhang, M., Han, Z., Wang, H., Yang, X., Wang, L."A novel four-step system for screening angiogenesis inhibitors". Molecular Medicine Reports 8.6 (2013): 1734-1740.
Chicago
Zhou, Q., Qi, C., Li, Y., He, X., Li, J., Zhang, Q., Tian, L., Zhang, M., Han, Z., Wang, H., Yang, X., Wang, L."A novel four-step system for screening angiogenesis inhibitors". Molecular Medicine Reports 8, no. 6 (2013): 1734-1740. https://doi.org/10.3892/mmr.2013.1704
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team