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Article

TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN

  • Authors:
    • Shihai Liu
    • Junfeng Sun
    • Qing Lan
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu 215004, P.R. China, Department of Surgical Oncology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China
  • Pages: 1741-1746
    |
    Published online on: October 1, 2013
       https://doi.org/10.3892/mmr.2013.1709
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Abstract

Human gliomas are associated with high rates of morbidity and mortality. In the brain, increased mRNA levels of transforming growth factor β (TGF‑β) correlate with the degree of malignancy of human gliomas. miR10a/10b expression has been demonstrated to be associated with TGF‑β expression in brain tumors, and it is reported that TGF‑β induces miR10 expression. Therefore, miR10a/10b expression may be induced by TGF‑β expression and may be involved in the TGF‑β‑induced migration of brain tumor cells. The present study examined the expression of TGF‑β and miR10a/10b in the tissues of 10 patients with brain tumors using quantitative PCR (qPCR), and the correlation between TGF‑β and miR10a or miR10b expression was analyzed. Additionally, U251 and SHG‑44 cells were treated with TGF‑β and the expression of miR10a/10b was examined. Further, cell migration was analyzed following transfection of U251 cells with miR10a/10b and the association between miR10a/10b and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was investigated. U251 cells were transfected with miR10a/10b inhibitors and a PTEN expression plasmid prior to TGF‑β treatment and then cell migration was assessed. A significant correlation was identified between TGF‑β and miR10a expression (r2=0.6936, P=0.007) and between TGF‑β and miR10b expression (r2=0.5876, P=0.02) in the tissues of patients with brain tumors. The results also showed that TGF‑β induces miR10a/10b expression and that TGF‑β‑induced miR10a/10b expression promotes cell migration through the suppression of PTEN. In conclusion, TGF‑β‑induced miR10a/10b promotes brain tumor migration. This study may provide a number of suggestions for the clinical treatment of brain tumors.
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1 

Chan XH, Nama S, Gopal F, Rizk P, Ramasamy S, Sundaram G, et al: Targeting glioma stem cells by functional inhibition of a prosurvival oncomiR-138 in malignant gliomas. Cell Rep. 2:591–602. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Zhang X, Yang H, Gong B, Jiang C and Yang L: Combined gene expression and protein interaction analysis of dynamic modularity in glioma prognosis. J Neurooncol. 107:281–288. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Mathupala SP, Mittal S, Guthikonda M and Sloan AE: MicroRNA and brain tumors: a cause and a cure? DNA Cell Biol. 26:301–310. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Fu H, Hu ZL, Wen JF, Wang KS and Liu Y: TGF-β promotes invasion and metastasis of gastric cancer cells by increasing fascin1 expression via ERK and JNK signal pathways. Acta Biochim Biophys Sin (Shanghai). 41:648–656. 2009.

5 

Kaminska B, Kocyk M and Kijewska M: TGF beta signaling and its role in glioma pathogenesis. Adv Exp Med Biol. 986:171–187. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Connolly EC, Freimuth J and Akhurst RJ: Complexities of TGF-β targeted cancer therapy. Int J Biol Sci. 8:964–978. 2012.

7 

Nicoloso MS and Calin GA: MicroRNA involvement in brain tumors: from bench to bedside. Brain Pathol. 18:122–129. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Hwang HW and Mendell JT: MicroRNAs in cell proliferation, cell death, and tumorigenesis. Br J Cancer. 94:776–780. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Gaur A, Jewell DA, Liang Y, Ridzon D, Moore JH, Chen C, Ambros VR and Israel MA: Characterization of microRNA expression levels and their biological correlates in human cancer cell lines. Cancer Res. 67:2456–2468. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Pang JC, Kwok WK, Chen Z and Ng HK: Oncogenic role of microRNAs in brain tumors. Acta Neuropathol. 117:599–611. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Mansfield JH, Harfe BD, Nissen R, Obenauer J, Srineel J, Chaudhuri A, et al: MicroRNA-responsive ‘sensor’ transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet. 36:1079–1083. 2004.

12 

Garzon R, Pichiorri F, Palumbo T, Iuliano R, Cimmino A, Aqeilan R, et al: MicroRNA fingerprints during human megakaryocytopoiesis. Proc Natl Acad Sci USA. 103:5078–5083. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Yan Y, Luo YC, Wan HY, et al: MicroRNA-10a is involved in the metastatic process by regulating Eph tyrosine kinase receptor A4-mediated epithelial-mesenchymal transition and adhesion in hepatoma cells. Hepatology. 57:667–677. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Weiss FU, Marques IJ, Woltering JM, Vlecken DH, Aghdassi A, Partecke LI, et al: Retinoic acid receptor antagonists inhibit miR-10a expression and block metastatic behaviour of pancreatic cancer. Gastroenterology. 137:2136–2145. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Ørom UA, Nielsen FC and Lund AH: MicroRNA-10a binds the 5′UTR of ribosomal protein mRNAs and enhances their translation. Mol Cell. 30:460–471. 2008.PubMed/NCBI

16 

Ma L, Teruya-Feldstein J and Weinberg RA: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 449:682–688. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Liu Z, Zhu J, Cao H, Ren H and Fang X: miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer. Int J Oncol. 40:1553–60. 2012.PubMed/NCBI

18 

Tian Y, Luo A, Cai Y, Su Q, Ding F, Chen H and Liu Z: MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. J Biol Chem. 285:7986–7994. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Sasayama T, Nishihara M, Kondoh T, Hosoda K and Kohmura E: MicroRNA-10b is overexpressed in malignant glioma and associated with tumor invasive factors, uPAR and RhoC. Int J Cancer. 125:1407–1413. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Garzon R, Garofalo M, Martelli MP, Briesewitz R, Wang L, Fernandez-Cymering C, et al: Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin. Proc Natl Acad Sci USA. 105:3945–3950. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Lund AH: miR-10 in development and cancer. Cell Death Differ. 17:209–214. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Takahashi H, Kanno T, Nakayamada S, Hirahara K, Sciumè G, Muljo SA, et al: TGF-β and retinoic acid induce the microRNA miR-10a, which targets Bcl-6 and constrains the plasticity of helper T cells. Nat Immunol. 13:587–595. 2012.

23 

Luo H, Yang Y, Duan J, Wu P, Jiang Q and Xu C: PTEN-regulated AKT/FoxO3a/Bim signaling contributes to reactive oxygen species-mediated apoptosis in selenite-treated colorectal cancer cells. Cell Death Dis. 4:e4812013. View Article : Google Scholar : PubMed/NCBI

24 

Wakefield LM, Letterio JJ, Chen T, Danielpour D, Allison RS, Pai LH, Denicoff AM, Noone MH, Cowan KH, O’Shaughnessy JA, et al: Transforming growth factor-beta1 circulates in normal human plasma and is unchanged in advanced metastatic breast cancer. Clin Cancer Res. 1:129–136. 1995.PubMed/NCBI

25 

Patel RR and Mehta MP: Targeted therapy for brain metastases: improving the therapeutic ratio. Clin Cancer Res. 13:1675–1683. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Heldin CH, Vanlandewijck M and Moustakas A: Regulation of EMT by TGF-β in cancer. FEBS Letters. 586:1959–1970. 2012.

27 

Wang B, Hsu SH, Majumder S, Kutay H, Huang W, Jacob ST and Ghosal K: TGFbeta-mediated upregulation of hepatic miR-181b promotes hepatocarcinogenesis by targeting TIMP3. Oncogene. 29:1787–1797. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Chung AC, Huang XR, Meng X and Lan HY: miR-192 mediates TGF-beta/Smad3-driven renal fibrosis. J Am Soc Nephrol. 21:1317–1325. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Dey N, Ghosh-Choudhury N, Kasinath BS and Choudhury GG: TGFβ-stimulated microRNA-21 utilizes PTEN to orchestrate AKT/mTORC1 signaling for mesangial cell hypertrophy and matrix expansion. PLoS One. 7:e423162012.

30 

Tian Y, Luo A, Cai Y, Su Q, Ding F, Chen H and Liu Z: MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. J Biol Chem. 285:7986–7994. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Chai G, Liu N, Ma J, Li H, Oblinger JL, Prahalad AK, et al: MicroRNA-10b regulates tumorigenesis in neurofibromatosis type 1. Cancer Sci. 101:1997–2004. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Zhang BG, Li JF, Yu BQ, Zhu ZG, Liu BY and Yan M: microRNA-21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN. Oncol Rep. 27:1019–1026. 2012.PubMed/NCBI

33 

Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST and Patel T: MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology. 133:647–658. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Sun J and Lan Q: TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN. Mol Med Rep 8: 1741-1746, 2013.
APA
Liu, S., Sun, J., & Lan, Q. (2013). TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN. Molecular Medicine Reports, 8, 1741-1746. https://doi.org/10.3892/mmr.2013.1709
MLA
Liu, S., Sun, J., Lan, Q."TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN". Molecular Medicine Reports 8.6 (2013): 1741-1746.
Chicago
Liu, S., Sun, J., Lan, Q."TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN". Molecular Medicine Reports 8, no. 6 (2013): 1741-1746. https://doi.org/10.3892/mmr.2013.1709
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Sun J and Lan Q: TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN. Mol Med Rep 8: 1741-1746, 2013.
APA
Liu, S., Sun, J., & Lan, Q. (2013). TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN. Molecular Medicine Reports, 8, 1741-1746. https://doi.org/10.3892/mmr.2013.1709
MLA
Liu, S., Sun, J., Lan, Q."TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN". Molecular Medicine Reports 8.6 (2013): 1741-1746.
Chicago
Liu, S., Sun, J., Lan, Q."TGF-β‑induced miR10a/b expression promotes human glioma cell migration by targeting PTEN". Molecular Medicine Reports 8, no. 6 (2013): 1741-1746. https://doi.org/10.3892/mmr.2013.1709
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