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Molecular Medicine Reports
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December 2013 Volume 8 Issue 6

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Article

Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation

  • Authors:
    • Masato Tamura
    • Maki Uyama
    • Yuri Sugiyama
    • Mari Sato
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, Kita‑ku, Sapporo 060-8586, Japan
  • Pages: 1807-1811
    |
    Published online on: October 4, 2013
       https://doi.org/10.3892/mmr.2013.1713
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Abstract

The canonical Wnt signaling pathway is crucial for the regulation of bone mass in humans and for the development of osteoblasts. MicroRNAs (miRs) represent a class of non‑coding RNAs, ~22 nucleotides in length, that regulate gene expression by targeting mRNAs for cleavage or translational repression. Several previous studies have demonstrated the involvement of miRNAs in modulating gene expression in osteoblasts and regulating osteoblast differentiation. In the present study, microRNA profiling was conducted using Wnt3a‑C2C12 cells; C2C12 cells were transfected with a Wnt3a expression plasmid to activate canonical Wnt signaling. miR‑34b‑5p and miR‑34c were identified to be upregulated by the activation of canonical Wnt signaling in C2C12 cells. Expression of mature miR‑34b/c increased from low levels at day 0 to maximum levels at day 28 of MC3T3‑E1 cell differentiation. To analyze the effects of these miRNAs on osteoblast differentiation, an antisense inhibitor was transfected into MC3T3‑E1 cells and osteoblast‑related gene expression was investigated. Knockdown of miR34b/c enhanced osteocalcin mRNA expression; however, alkaline phosphatase mRNA expression and activity were decreased by miR34b/c inhibition. These results indicated that miR‑34b/c regulates gene expression by targeting regulators of the osteogenic pathways and thereby contributes to osteoblast differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Tamura M, Uyama M, Sugiyama Y and Sato M: Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation. Mol Med Rep 8: 1807-1811, 2013.
APA
Tamura, M., Uyama, M., Sugiyama, Y., & Sato, M. (2013). Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation. Molecular Medicine Reports, 8, 1807-1811. https://doi.org/10.3892/mmr.2013.1713
MLA
Tamura, M., Uyama, M., Sugiyama, Y., Sato, M."Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation". Molecular Medicine Reports 8.6 (2013): 1807-1811.
Chicago
Tamura, M., Uyama, M., Sugiyama, Y., Sato, M."Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation". Molecular Medicine Reports 8, no. 6 (2013): 1807-1811. https://doi.org/10.3892/mmr.2013.1713
Copy and paste a formatted citation
x
Spandidos Publications style
Tamura M, Uyama M, Sugiyama Y and Sato M: Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation. Mol Med Rep 8: 1807-1811, 2013.
APA
Tamura, M., Uyama, M., Sugiyama, Y., & Sato, M. (2013). Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation. Molecular Medicine Reports, 8, 1807-1811. https://doi.org/10.3892/mmr.2013.1713
MLA
Tamura, M., Uyama, M., Sugiyama, Y., Sato, M."Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation". Molecular Medicine Reports 8.6 (2013): 1807-1811.
Chicago
Tamura, M., Uyama, M., Sugiyama, Y., Sato, M."Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation". Molecular Medicine Reports 8, no. 6 (2013): 1807-1811. https://doi.org/10.3892/mmr.2013.1713
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