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Article

Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media

  • Authors:
    • Xiu M. Guan
    • Min Cheng
    • Hong Li
    • Xiao D. Cui
    • Xin Li
    • Yu L. Wang
    • Jin L. Sun
    • Xiao Y. Zhang
  • View Affiliations / Copyright

    Affiliations: Medicine Research Center, Weifang Medical University, Weifang, Shandong 261053, P.R. China
  • Pages: 1722-1728
    |
    Published online on: October 9, 2013
       https://doi.org/10.3892/mmr.2013.1718
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Abstract

Ex vivo expansion of endothelial progenitor cells (EPCs) may be a promising strategy to overcome the clinical problem of limited cell numbers. As the culture medium is the key for the cell characteristics, the effects of different culture media on EPCs were investigated in the present study. Rat bone marrow mononuclear cells were cultured in different media, including M-199 media with 20% fetal bovine serum (FBS) and bovine pituitary extract (M1); M-199 media with 10% FBS, 20 ng/ml vascular endothelial growth factor (VEGF) and 10 ng/ml basic fibroblast growth factor (bFGF; M2) or epidermal growth medium (EGM)-2MV media. The cell morphology and biological functions, such as proliferation, adhesion, migration, tube formation and nitric oxide (NO) production were subsequently assayed in vitro. Moreover, endothelial biomarkers and apoptosis were also analyzed. The results showed that endothelial‑like cells appeared in all of the culture systems. First‑passage cells, namely early EPCs, tended to form colonies in M2 and EGM-2MV media but showed a fusiform shape in M1 media. The 3rd or 4th generation EPCs, namely late EPCs, cultured in EGM-2MV media exhibited increased adhesion, migration, tube formation and NO production as compared with EPCs in M1 or M2 media. Furthermore, late EPCs cultured in EGM-2MV expressed higher levels of endothelial cell markers, such as von Willibrand factor (vWF)and CD31, but relatively greater levels of apoptosis were observed. In conclusion, cell culture conditions, for example the medium used, affects the biological properties of bone marrow-derived early and late EPCs.
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1 

Vita JA: Endothelial function. Circulation. 124:e906–e912. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Möbius-Winkler S, Höllriegel R, Schuler G and Adams V: Endothelial progenitor cells: implications for cardiovascular disease. Cytometry A. 75:25–37. 2009.

3 

Ben-Shoshan J and George J: Endothelial progenitor cells as therapeutic vectors in cardiovascular disorders: from experimental models to human trials. Pharmacol Ther. 115:25–36. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Urbich C, Aicher A, Heeschen C, et al: Soluble factors released by endothelial progenitor cells promote migration of endothelial cells and cardiac resident progenitor cells. J Mol Cell Cardiol. 39:733–742. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Wang TJ, Yang YJ, Xu B, et al: Atorvastatin accelerates both neointimal coverage and re-endothelialization after sirolimus-eluting stent implantation in a porcine model: new findings from optical coherence tomography and pathology. Circ J. 76:2561–2571. 2012. View Article : Google Scholar

6 

Hur J, Yoon CH, Kim HS, et al: Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 24:288–293. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Brown MA, Wallace CS, Angelos M and Truskey GA: Characterization of umbilical cord blood-derived late outgrowth endothelial progenitor cells exposed to laminar shear stress. Tissue Eng Part A. 15:3575–3587. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Aburakawa Y, Kawabe J, Okada M, et al: Prostacyclin stimulated integrin-dependent angiogenic effects of endothelial progenitor cells and mediated potent circulation recovery in ischemic hind limb model. Circ J. 77:1053–1062. 2013. View Article : Google Scholar

9 

Iwaguro H, Yamaguchi J, Kalka C, et al: Endothelial progenitor cell vascular endothelial growth factor gene transfer for vascular regeneration. Circulation. 105:732–738. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Taniguchi E, Kin M, Torimura T, et al: Endothelial progenitor cell transplantation improves the survival following liver injury in mice. Gastroenterology. 130:521–531. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Loomans CJ, de Koning EJ, Staal FJ, et al: Endothelial progenitor cell dysfunction: a novel concept in the pathogenesis of vascular complications of type 1 diabetes. Diabetes. 53:195–199. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Yang N, Li D, Jiao P, et al: The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions. Cytotechnology. 63:217–226. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Ye C, Bai L, Yan ZQ, Wang YH and Jiang ZL: Shear stress and vascular smooth muscle cells promote endothelial differentiation of endothelial progenitor cells via activation of Akt. Clin Biomech (Bristol, Avon). 23(Suppl 1): S118–S124. 2008. View Article : Google Scholar

14 

Zhang X, Cui X, Cheng L, et al: Actin stabilization by jasplakinolide affects the function of bone marrow-derived late endothelial progenitor cells. PLoS One. 7:e508992012. View Article : Google Scholar : PubMed/NCBI

15 

Tepper OM, Galiano RD, Capla JM, et al: Human endothelial progenitor cells from type II diabetics exhibit impaired proliferation, adhesion, and incorporation into vascular structures. Circulation. 106:2781–2786. 2002. View Article : Google Scholar

16 

Chen YH, Lin SJ, Lin FY, et al: High glucose impairs early and late endothelial progenitor cells by modifying nitric oxide-related but not oxidative stress-mediated mechanisms. Diabetes. 56:1559–1568. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Jianguo W, Tianhang L, Hong Z, et al: Optimization of culture conditions for endothelial progenitor cells from porcine bone marrow in vitro. Cell Prolif. 43:418–426. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Cui X, Zhang X, Guan X, et al: Shear stress augments the endothelial cell differentiation marker expression in late EPCs by upregulating integrins. Biochem Biophys Res Commun. 425:419–425. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Urbich C and Dimmeler S: Endothelial progenitor cells: characterization and role in vascular biology. Circ Res. 95:343–353. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Yoon CH, Hur J, Park KW, et al: Synergistic neovascularization by mixed transplantation of early endothelial progenitor cells and late outgrowth endothelial cells: the role of angiogenic cytokines and matrix metalloproteinases. Circulation. 112:1618–1627. 2005. View Article : Google Scholar

21 

Hamed S, Brenner B and Roguin A: Nitric oxide: a key factor behind the dysfunctionality of endothelial progenitor cells in diabetes mellitus type-2. Cardiovasc Res. 91:9–15. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Asahara T, Murohara T, Sullivan A, et al: Isolation of putative progenitor endothelial cells for angiogenesis. Science. 275:964–967. 1997. View Article : Google Scholar : PubMed/NCBI

23 

Wang QR, Wang BH, Huang YH, Dai G, Li WM and Yan Q: Purification and growth of endothelial progenitor cells from murine bone marrow mononuclear cells. J Cell Biochem. 103:21–29. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Muscari C, Gamberini C, Basile I, et al: Comparison between culture conditions improving growth and differentiation of blood and bone marrow cells committed to the endothelial cell lineage. Biol Proced Online. 12:90232010. View Article : Google Scholar : PubMed/NCBI

25 

Yang J, Ii M, Kamei N, et al: CD34+ cells represent highly functional endothelial progenitor cells in murine bone marrow. PLoS One. 6:e202192011.

26 

Ramasamy R, Tong CK, Yip WK, Vellasamy S, Tan BC and Seow HF: Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. Cell Prolif. 45:132–139. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Desjardins F and Balligand JL: Nitric oxide-dependent endothelial function and cardiovascular disease. Acta Clin Belg. 61:326–334. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Ozüyaman B, Ebner P, Niesler U, et al: Nitric oxide differentially regulates proliferation and mobilization of endothelial progenitor cells but not of hematopoietic stem cells. Thromb Haemost. 94:770–772. 2005.PubMed/NCBI

29 

He T, Peterson TE, Holmuhamedov EL, et al: Human endothelial progenitor cells tolerate oxidative stress due to intrinsically high expression of manganese superoxide dismutase. Arterioscler Thromb Vasc Biol. 24:2021–2027. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guan XM, Cheng M, Li H, Cui XD, Li X, Wang YL, Sun JL and Zhang XY: Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Mol Med Rep 8: 1722-1728, 2013.
APA
Guan, X.M., Cheng, M., Li, H., Cui, X.D., Li, X., Wang, Y.L. ... Zhang, X.Y. (2013). Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Molecular Medicine Reports, 8, 1722-1728. https://doi.org/10.3892/mmr.2013.1718
MLA
Guan, X. M., Cheng, M., Li, H., Cui, X. D., Li, X., Wang, Y. L., Sun, J. L., Zhang, X. Y."Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media". Molecular Medicine Reports 8.6 (2013): 1722-1728.
Chicago
Guan, X. M., Cheng, M., Li, H., Cui, X. D., Li, X., Wang, Y. L., Sun, J. L., Zhang, X. Y."Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media". Molecular Medicine Reports 8, no. 6 (2013): 1722-1728. https://doi.org/10.3892/mmr.2013.1718
Copy and paste a formatted citation
x
Spandidos Publications style
Guan XM, Cheng M, Li H, Cui XD, Li X, Wang YL, Sun JL and Zhang XY: Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Mol Med Rep 8: 1722-1728, 2013.
APA
Guan, X.M., Cheng, M., Li, H., Cui, X.D., Li, X., Wang, Y.L. ... Zhang, X.Y. (2013). Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Molecular Medicine Reports, 8, 1722-1728. https://doi.org/10.3892/mmr.2013.1718
MLA
Guan, X. M., Cheng, M., Li, H., Cui, X. D., Li, X., Wang, Y. L., Sun, J. L., Zhang, X. Y."Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media". Molecular Medicine Reports 8.6 (2013): 1722-1728.
Chicago
Guan, X. M., Cheng, M., Li, H., Cui, X. D., Li, X., Wang, Y. L., Sun, J. L., Zhang, X. Y."Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media". Molecular Medicine Reports 8, no. 6 (2013): 1722-1728. https://doi.org/10.3892/mmr.2013.1718
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