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December 2013 Volume 8 Issue 6

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Article

Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1

  • Authors:
    • Xinyu Liu
    • Xianglu Li
    • Ling Wang
    • Xiao Lv
    • Na Chen
    • Peipei Li
    • Kang Lu
    • Xin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 1866-1870
    |
    Published online on: October 14, 2013
       https://doi.org/10.3892/mmr.2013.1731
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Abstract

The aim of the present study was to investigate the effect of realgar on the viability, proliferation and apoptosis in the human chronic lymphocytic leukemia (CLL) cell line, MEC‑1. Potential mechanisms mediating the effect were also explored in the experiment. Cultured MEC‑1 cells were incubated with various concentrations of realgar for 24, 48 and 72 h. A WST‑8 assay was employed to evaluate the effect on cell viability. Inhibitory effects on cell proliferation were determined using a 5‑bromodeoxyuridine cell proliferation ELISA. The apoptotic effect on MEC‑1 cells was evaluated by annexin V‑fluorescein isothiocyanate/propidium iodide dual staining, followed by flow cytometry. Quantitative polymerase chain reaction was performed to determine the mRNA expression levels of BCL2‑associated X protein (BAX), BCL2‑like 1 (Bcl-xL), v‑myc myelocytomatosis viral oncogene homolog (avian; c‑Myc) and cyclin‑dependent kinase inhibitor 1A (p21). It was found that viability and proliferation were significantly reduced while apoptotic rates increased in MEC‑1 cells following exposure to realgar. Furthermore, mRNA expression of BAX and c‑Myc was upregulated and downregulated, respectively, in realgar‑treated MEC‑1 cells. In conclusion, the results showed that realgar inhibits viability and prolife­ration and induces apoptosis of MEC‑1 cells in a dose‑ and time‑dependent manner. The effect may depend on the mitochondrial apoptosis pathway. The results of the present study may be beneficial in the identification of a new target therapy for CLL.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Li X, Wang L, Lv X, Chen N, Li P, Lu K and Wang X: Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1. Mol Med Rep 8: 1866-1870, 2013.
APA
Liu, X., Li, X., Wang, L., Lv, X., Chen, N., Li, P. ... Wang, X. (2013). Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1. Molecular Medicine Reports, 8, 1866-1870. https://doi.org/10.3892/mmr.2013.1731
MLA
Liu, X., Li, X., Wang, L., Lv, X., Chen, N., Li, P., Lu, K., Wang, X."Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1". Molecular Medicine Reports 8.6 (2013): 1866-1870.
Chicago
Liu, X., Li, X., Wang, L., Lv, X., Chen, N., Li, P., Lu, K., Wang, X."Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1". Molecular Medicine Reports 8, no. 6 (2013): 1866-1870. https://doi.org/10.3892/mmr.2013.1731
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Li X, Wang L, Lv X, Chen N, Li P, Lu K and Wang X: Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1. Mol Med Rep 8: 1866-1870, 2013.
APA
Liu, X., Li, X., Wang, L., Lv, X., Chen, N., Li, P. ... Wang, X. (2013). Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1. Molecular Medicine Reports, 8, 1866-1870. https://doi.org/10.3892/mmr.2013.1731
MLA
Liu, X., Li, X., Wang, L., Lv, X., Chen, N., Li, P., Lu, K., Wang, X."Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1". Molecular Medicine Reports 8.6 (2013): 1866-1870.
Chicago
Liu, X., Li, X., Wang, L., Lv, X., Chen, N., Li, P., Lu, K., Wang, X."Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC‑1". Molecular Medicine Reports 8, no. 6 (2013): 1866-1870. https://doi.org/10.3892/mmr.2013.1731
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