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Article

Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus

  • Authors:
    • Jun Fu
    • Rui Xue
    • Junfeng Gu
    • Yun Xiao
    • Heying Zhong
    • Xuelian Pan
    • Ran Ran
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 1708-1714
    |
    Published online on: October 14, 2013
       https://doi.org/10.3892/mmr.2013.1734
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Abstract

Calcitriol has been demonstrated to provide neuroprotection against ischemia/reperfusion (I/R) injury. However, the exact mechanism of this protection remains unknown. In the present study, the neuroprotective effect of calcitriol was investigated in rats exposed to cerebral I/R injury induced by middle cerebral artery occlusion (MCAO). In addition, the involvement of NR3A, extracellular signal‑regulated kinase 1/2 (ERK1/2), and phosphorylated cAMP/Ca2+‑response element binding protein (p‑CREB) in this protective action was determined in the hippocampal neurons. Western blot analysis was conducted to analyze the protein levels of NR3A, mitogen‑activated protein kinase kinase (MEK) and p‑CREB. The immunoreactivity of p‑CREB and NR3A were measured by quantum dot‑based immunofluorescence analysis. Results showed that MCAO rats exhibited large cortical infarct volumes. By contrast, intraperitoneal administration of calcitriol significantly reduced infarct volumes seven days following reperfusion, and these results were accompanied by elevated NR3A and p‑CREB activity in the hippocampal neurons. The inhibition of MEK by the addition of PD98059 led to attenuation of the neuroprotective effects of calcitriol and a correlated decrease in CREB activity. The results also demonstrated that calcitriol protected the brain from I/R injury through the NR3A‑MEK/ERK‑CREB pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fu J, Xue R, Gu J, Xiao Y, Zhong H, Pan X and Ran R: Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Mol Med Rep 8: 1708-1714, 2013.
APA
Fu, J., Xue, R., Gu, J., Xiao, Y., Zhong, H., Pan, X., & Ran, R. (2013). Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Molecular Medicine Reports, 8, 1708-1714. https://doi.org/10.3892/mmr.2013.1734
MLA
Fu, J., Xue, R., Gu, J., Xiao, Y., Zhong, H., Pan, X., Ran, R."Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus". Molecular Medicine Reports 8.6 (2013): 1708-1714.
Chicago
Fu, J., Xue, R., Gu, J., Xiao, Y., Zhong, H., Pan, X., Ran, R."Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus". Molecular Medicine Reports 8, no. 6 (2013): 1708-1714. https://doi.org/10.3892/mmr.2013.1734
Copy and paste a formatted citation
x
Spandidos Publications style
Fu J, Xue R, Gu J, Xiao Y, Zhong H, Pan X and Ran R: Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Mol Med Rep 8: 1708-1714, 2013.
APA
Fu, J., Xue, R., Gu, J., Xiao, Y., Zhong, H., Pan, X., & Ran, R. (2013). Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Molecular Medicine Reports, 8, 1708-1714. https://doi.org/10.3892/mmr.2013.1734
MLA
Fu, J., Xue, R., Gu, J., Xiao, Y., Zhong, H., Pan, X., Ran, R."Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus". Molecular Medicine Reports 8.6 (2013): 1708-1714.
Chicago
Fu, J., Xue, R., Gu, J., Xiao, Y., Zhong, H., Pan, X., Ran, R."Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus". Molecular Medicine Reports 8, no. 6 (2013): 1708-1714. https://doi.org/10.3892/mmr.2013.1734
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