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Article

Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes

  • Authors:
    • Biswajit Podder
    • Yong-Sik Kim
    • Ho-Yeon Song
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan, Chungnam 330-721, Republic of Korea
  • Pages: 1852-1860
    |
    Published online on: October 16, 2013
       https://doi.org/10.3892/mmr.2013.1736
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Abstract

The extract of sea buckthorn (SBT) [Hippophae rhamnoides L. (Elaeagnaceae)], is used as a food supplement and traditional medicine in numerous countries. This study investigated the protective effects of the functional extract of SBT against paraquat (PQ)‑induced toxicity via antioxidant mechanisms in A549 cells. The methanol extract of SBT (25‑200 µg/ml) was used to protect cells against PQ (200 µM)‑induced cell death. A viability assay was conducted using 3‑(4,5‑dimethylthioazol‑2‑ly)‑2,5‑diphenyltetrazolium bromide and lactate dehydrogenase (LDH). Total intracellular reactive oxygen species (ROS) were measured and plotted. For validation of the SBT‑induced expression of nuclear factor‑E2‑related factor 2 (Nrf2) and its target genes, western blot analysis and qPCR were performed. The present study showed that pretreatment of A549 cells with SBT extract significantly attenuated PQ (200 µM)‑induced cellular toxicity. The maximum cytoprotective effect was identified using 200 µg/ml SBT extract; it began 24 h following exposure and was sustained up to 120 h (P<0.05). SBT extract significantly reduced LDH activity by 35.63% and ROS levels by 30.90% (P<0.05). Pretreatment with SBT extract activated Nrf2 mRNA and protein expression and its nuclear translocation. The SBT extract effectively induced Nrf2 target genes, such as NAD(P)H dehydrogenase quinone 1, glutathione peroxidase 1, glutathione reductase and catalase following treatment with PQ. Based on these results, it was hypothesized that SBT extract may be used as a potential therapeutic agent for the treatment of various oxidative stress‑related diseases.
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1 

Schuster BG: Demonstrating the validity of natural products as anti-infective drugs. J Altern Complement Med. 7(Supp 1): S73–S82. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Nocentini S, Guggiari M, Rouillard D and Surgis S: Exacerbating effect of vitamin E supplementation on DNA damage induced in cultured human normal fibroblasts by UVA radiation. Photochem Photobiol. 73:370–377. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Kumar R, Shyam R, Divekar HM, Pahwa ML and Srivastava KK: Mechanism of increased tolerance to hypothermia after composite Indian herbal preparation II administration. J Altern Complement Med. 6:509–517. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Duarte J, Andriambeloson E, Diebolt M and Andriantsitohaina R: Wine polyphenols stimulate superoxide anion production to promote calcium signaling and endothelial-dependent vasodilatation. Physiol Res. 53:595–602. 2004.

5 

Guliyev VB, Gul M and Yildirim A: Hippophae rhamnoides L.: chromatographic methods to determine chemical composition, use in traditional medicine and pharmacological effects. J Chromatogr B Analyt Technol Biomed Life Sci. 812:291–307. 2004. View Article : Google Scholar

6 

Suryakumar G and Gupta A: Medicinal and therapeutic potential of sea buckthorn (Hippophae rhamnoides L.). J Ethnopharmacol. 138:268–278. 2011. View Article : Google Scholar

7 

Kim JS, Kwon YS, Sa YJ and Kim MJ: Isolation and identification of sea buckthorn (Hippophae rhamnoides) phenolics with antioxidant activity and alpha-glucosidase inhibitory effect. J Agric Food Chem. 59:138–144. 2011.

8 

Saggu S and Kumar R: Effect of seabuckthorn (Hippophae rhamnoides) leaf aqueous and ethanol extracts on avoidance learning during stressful endurance performance of rats: a dose dependent study. Phytother Res. 22:1183–1187. 2008.

9 

Padwad Y, Ganju L, Jain M, et al: Effect of leaf extract of Seabuckthorn on lipopolysaccharide induced inflammatory response in murine macrophages. Int Immunopharmacol. 6:46–52. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Maheshwari DT, Yogendra Kumar MS, Verma SK, Singh VK and Singh SN: Antioxidant and hepatoprotective activities of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves. Food Chem Toxicol. 49:2422–2428. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Bao M and Lou Y: Flavonoids from seabuckthorn protect endothelial cells (EA. hy926) from oxidized low-density lipoprotein induced injuries via regulation of LOX-1 and eNOS expression. J Cardiovasc Pharmacol. 48:834–841. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Kurata S, Matsumoto M, Tsuji Y and Nakajima H: Lipopolysaccharide activates transcription of the heme oxygenase gene in mouse M1 cells through oxidative activation of nuclear factor kappa B. Eur J Biochem. 239:566–571. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Suntres ZE: Role of antioxidants in paraquat toxicity. Toxicology. 180:65–77. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Bus JS and Gibson JE: Paraquat: model for oxidant-initiated toxicity. Environ Health Perspect. 55:37–46. 1984. View Article : Google Scholar : PubMed/NCBI

15 

Ghio AJ: Disruption of iron homeostasis and lung disease. Biochim Biophys Acta. 1790:731–739. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Mitsopoulos P and Suntres ZE: Cytotoxicity and gene array analysis of alveolar epithelial A549 cells exposed to paraquat. Chem Biol Interact. 188:427–436. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Zerin T, Kim YS, Hong SY and Song HY: Quercetin reduces oxidative damage induced by paraquat via modulating expression of antioxidant genes in A549 cells. J Appl Toxicol. Sep 20–2012.(Epub ahead of print).

18 

Geetha S, Singh V, Ram MS, Ilavazhagan G, Banerjee PK and Sawhney RC: Immunomodulatory effects of seabuckthorn (Hippophae rhamnoides L.) against chromium (VI) induced immunosuppression. Mol Cell Biochem. 278:101–109. 2005.

19 

Geetha S, Sai Ram M, Singh V, Ilavazhagan G and Sawhney RC: Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides) - an in vitro study. J Ethnopharmacol. 79:373–378. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Narayanan S, Ruma D, Gitika B, et al: Antioxidant activities of seabuckthorn (Hippophae rhamnoides) during hypoxia induced oxidative stress in glial cells. Mol Cell Biochem. 278:9–14. 2005.

21 

Upadhyay NK, Kumar R, Mandotra SK, et al: Safety and healing efficacy of Sea buckthorn (Hippophae rhamnoides L.) seed oil on burn wounds in rats. Food Chem Toxicol. 47:1146–1153. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Ganju L, Padwad Y, Singh R, et al: Anti-inflammatory activity of seabuckthorn (Hippophae rhamnoides) leaves. Int Immunopharmacol. 5:1675–1684. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Chen CH, Liu TZ, Kuo TC, et al: Casuarinin protects cultured MDCK cells from hydrogen peroxide-induced oxidative stress and DNA oxidative damage. Planta Med. 70:1022–1026. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Kwon DJ, Bae YS, Ju SM, Goh AR, Choi SY and Park J: Casuarinin suppresses TNF-alpha-induced ICAM-1 expression via blockade of NF-κB activation in HaCaT cells. Biochem Biophys Res Commun. 409:780–785. 2011.PubMed/NCBI

25 

Zerin T, Kim YS, Hong SY and Song HY: Protective effect of methylprednisolone on paraquat-induced A549 cell cytotoxicity via induction of efflux transporter, P-glycoprotein expression. Toxicol Lett. 208:101–107. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Surh YJ, Kundu JK and Na HK: Nrf2 as a master redox switch in turning on the cellular signaling involved in the induction of cytoprotective genes by some chemopreventive phytochemicals. Planta Med. 74:1526–1539. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Jaiswal AK: Nrf2 signaling in coordinated activation of antioxidant gene expression. Free Radic Biol Med. 36:1199–1207. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Kode A, Rajendrasozhan S, Caito S, Yang SR, Megson IL and Rahman I: Resveratrol induces glutathione synthesis by activation of Nrf2 and protects against cigarette smoke-mediated oxidative stress in human lung epithelial cells. Am J Physiol Lung Cell Mol Physiol. 294:L478–L488. 2008. View Article : Google Scholar

29 

Cho HY, Jedlicka AE, Reddy SP, et al: Role of NRF2 in protection against hyperoxic lung injury in mice. Am J Respir Cell Mol Biol. 26:175–182. 2002. View Article : Google Scholar : PubMed/NCBI

30 

He X, Lin GX, Chen MG, Zhang JX and Ma Q: Protection against chromium (VI)-induced oxidative stress and apoptosis by Nrf2. Recruiting Nrf2 into the nucleus and disrupting the nuclear Nrf2/Keap1 association. Toxicol Sci. 98:298–309. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Chen J and Shaikh ZA: Activation of Nrf2 by cadmium and its role in protection against cadmium-induced apoptosis in rat kidney cells. Toxicol Appl Pharmacol. 241:81–89. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Singh M, Murthy V and Ramassamy C: Standardized extracts of Bacopa monniera protect against MPP+- and paraquat-induced toxicity by modulating mitochondrial activities, proteasomal functions, and redox pathways. Toxicol Sci. 125:219–232. 2012.

33 

Liu XP, Goldring CE, Copple IM, et al: Extract of Ginkgo biloba induces phase 2 genes through Keap1-Nrf2-ARE signaling pathway. Life Sci. 80:1586–1591. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Camhi SL, Alam J, Wiegand GW, Chin BY and Choi AM: Transcriptional activation of the HO-1 gene by lipopolysaccharide is mediated by 5′ distal enhancers: role of reactive oxygen intermediates and AP-1. Am J Respir Cell Mol Biol. 18:226–234. 1998.

35 

Fujii J, Ito JI, Zhang X and Kurahashi T: Unveiling the roles of the glutathione redox system in vivo by analyzing genetically modified mice. J Clin Biochem Nutr. 49:70–78. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Singh A, Boldin-Adamsky S, Thimmulappa RK, et al: RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapy. Cancer Res. 68:7975–7984. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Rangasamy T, Cho CY, Thimmulappa RK, et al: Genetic ablation of Nrf2 enhances susceptibility to cigarette smoke-induced emphysema in mice. J Clin Invest. 114:1248–1259. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Tomita M and Okuyama T: Effect of paraquat on the malondialdehyde level in rat liver microsomes (in vitro). Arch Toxicol. 68:187–192. 1994. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Podder B, Kim Y and Song H: Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes. Mol Med Rep 8: 1852-1860, 2013.
APA
Podder, B., Kim, Y., & Song, H. (2013). Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes. Molecular Medicine Reports, 8, 1852-1860. https://doi.org/10.3892/mmr.2013.1736
MLA
Podder, B., Kim, Y., Song, H."Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes". Molecular Medicine Reports 8.6 (2013): 1852-1860.
Chicago
Podder, B., Kim, Y., Song, H."Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes". Molecular Medicine Reports 8, no. 6 (2013): 1852-1860. https://doi.org/10.3892/mmr.2013.1736
Copy and paste a formatted citation
x
Spandidos Publications style
Podder B, Kim Y and Song H: Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes. Mol Med Rep 8: 1852-1860, 2013.
APA
Podder, B., Kim, Y., & Song, H. (2013). Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes. Molecular Medicine Reports, 8, 1852-1860. https://doi.org/10.3892/mmr.2013.1736
MLA
Podder, B., Kim, Y., Song, H."Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes". Molecular Medicine Reports 8.6 (2013): 1852-1860.
Chicago
Podder, B., Kim, Y., Song, H."Cytoprotective effect of bioactive sea buckthorn extract on paraquat-exposed A549 cells via induction of Nrf2 and its downstream genes". Molecular Medicine Reports 8, no. 6 (2013): 1852-1860. https://doi.org/10.3892/mmr.2013.1736
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