Capsaicin induces apoptosis in MG63 human osteosarcoma cells via the caspase cascade and the antioxidant enzyme system

Cho,Won  Ho; Lee,Hyun  Joo; Choi,Yoon  Ji; Oh,Joo  Han; Kim,Han  Soo; Cho,Hwan   Seong

doi:10.3892/mmr.2013.1737

Capsaicin induces apoptosis in MG63 human osteosarcoma cells via the caspase cascade and the antioxidant enzyme system

Authors:
- Won Ho Cho
- Hyun Joo Lee
- Yoon Ji Choi
- Joo Han Oh
- Han Soo Kim
- Hwan Seong Cho
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Affiliations: Department of Neurosurgery and Medical Research Institute, Pusan National University Hospital, Pusan National University School of Medicine, Busan 602739, Republic of Korea, Department of Orthopaedic Surgery, Seoul National University Bundang Hospital, Gyeonnggido 463707, Republic of Korea, Department of Orthopaedic Surgery, Seoul National University Hospital, Seoul 110744, Republic of Korea
Published online on: October 17, 2013 https://doi.org/10.3892/mmr.2013.1737
Pages: 1655-1662
Copyright: © Cho et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Osteosarcoma is the most common malignant bone tumor in children and adolescents. This aggressive cancer mostly occurs in the long bones. Therefore, novel therapeutic approaches, such as biological therapies and gene therapy, are required to efficiently treat osteosarcoma. Capsaicin (trans‑8‑methyl‑N‑vanillyl‑6‑nonenamide) has been demonstrated to inhibit the growth of several types of cancer cells and a number of studies have shown that osteosarcoma may be vulnerable to biological therapies. However, little is known regarding the therapeutic effects of capsaicin on osteosarcoma. This study investigated the effects of capsaicin on MG63 human osteosarcoma cells, in addition to elucidating the regulatory signaling pathways underlying the effects of capsaicin, the caspase cascade and the antioxidant enzyme system. The MG63 cell line was treated with various concentrations of capsaicin. Cells were analyzed using MTT and flow cytometry, and the presence of DNA fragmentation was evaluated using TUNEL assay. Results showed capsaicin induced apoptosis in MG63 cells. Thus, capsaicin exhibited an anticancer effect in osteosarcoma cells.

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