Altered expression of pannexin proteins in patients with temporal lobe epilepsy

Jiang,Ting; Long,Hongyu; Ma,Yan; Long,Lili; Li,Yi; Li,Feng; Zhou,Pinting; Yuan,Chunyun; Xiao,Bo

doi:10.3892/mmr.2013.1739

December 2013 Volume 8 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

December 2013 Volume 8 Issue 6

Full Size Image

Article

Altered expression of pannexin proteins in patients with temporal lobe epilepsy

Authors:
- Ting Jiang
- Hongyu Long
- Yan Ma
- Lili Long
- Yi Li
- Feng Li
- Pinting Zhou
- Chunyun Yuan
- Bo Xiao
View Affiliations / Copyright

Affiliations: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Pages: 1801-1806
|
Published online on: October 18, 2013

https://doi.org/10.3892/mmr.2013.1739
Expand metrics +

Abstract

The aim of the present study was to investigate the expression of the pannexin (Panx) proteins, Panx1 and Panx2, in the temporal lobe tissue of patients with temporal lobe epilepsy (TLE). Immunohistochemistry and western blotting methods were used to localize and quantify Panx1 and Panx2 in the surgically removed brain tissue of patients with TLE (n=37). The results were then compared with non-epileptogenic controls (n=9). Panx1 and Panx2 expression was detected in the temporal lobe cortex of patients with TLE and in the control tissues. Panx1 and Panx2 proteins were expressed in all layers of the epileptic cortex, but predominantly in layers II and III of the cortex in the control group. Panx1 protein expression was significantly higher in the temporal lobe cortex of the patients with TLE than in the controls (P<0.05; t-test); however, no significant differences were identified in the Panx2 expression levels between the patients and the controls (P>0.05; t-test). The expression of the two Panx proteins in the tissue layers of the epileptic cortex varied in the patients and controls. The results indicate that Panx channels may be involved in the pathogenesis of TLE.

View Figures

View References

1	Panchin Y, Kelmanson I, Matz M, Lukyanov K, Usman N and Lukyanov S: A ubiquitous family of putative gap junction molecules. Curr Biol. 10:R473–474. 2000. View Article : Google Scholar : PubMed/NCBI
2	Bruzzone R, Hormuzdi SG, Barbe MT, Herb A and Monyer H: Pannexins, a family of gap junction proteins expressed in brain. Proc Natl Acad Sci USA. 100:13644–13649. 2003. View Article : Google Scholar : PubMed/NCBI
3	Bruzzone R, White TW and Paul DL: Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem. 238:1–27. 1996. View Article : Google Scholar : PubMed/NCBI
4	White TW and Paul DL: Genetic diseases and gene knockouts reveal diverse connexin functions. Annu Rev Physiol. 61:283–310. 1999. View Article : Google Scholar : PubMed/NCBI
5	Vogt A, Hormuzdi SG and Monyer H: Pannexin1 and Pannexin2 expression in the developing and mature rat brain. Brain Res Mol Brain Res. 141:113–120. 2005. View Article : Google Scholar : PubMed/NCBI
6	Thompson RJ, Jackson MF, Olah ME, et al: Activation of pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science. 322:1555–1559. 2008. View Article : Google Scholar : PubMed/NCBI
7	Kawamura M Jr, Ruskin DN and Masino SA: Metabolic autocrine regulation of neurons involves cooperation among pannexin hemichannels, adenosine receptors, and KATP channels. J Neurosci. 30:3886–3895. 2010. View Article : Google Scholar
8	No authors listed. Proposal for Revised Clinical and Electroencephalographic Classification of Epileptic Seizures. Epilepsia. 22:489–501. 1981. View Article : Google Scholar : PubMed/NCBI
9	Yuan J, Wang LY, Li JM, et al: Altered expression of the small guanosine triphosphatase RhoA in human temporal lobe epilepsy. J Mol Neurosci. 42:53–58. 2010. View Article : Google Scholar : PubMed/NCBI
10	Zappalà A, Cicero D, Serapide MF, et al: Expression of pannexin1 in the CNS of adult mouse: cellular localization and effect of 4-aminopyridine-induced seizures. Neuroscience. 141:167–178. 2006.PubMed/NCBI
11	Zappalà A, Li Volti G, Serapide MF, et al: Expression of pannexin2 protein in healthy and ischemized brain of adult rats. Neuroscience. 148:653–667. 2007.PubMed/NCBI
12	Di Pasquale E, Keegan KD and Noebels JL: Increased excitability and inward rectification in layer V cortical pyramidal neurons in the epileptic mutant mouse Stargazer. J Neurophysiol. 77:621–631. 1997.PubMed/NCBI
13	Jin X, Prince DA and Huguenard JR: Enhanced excitatory synaptic connectivity in layer v pyramidal neurons of chronically injured epileptogenic neocortex in rats. J Neurosci. 26:4891–4900. 2006. View Article : Google Scholar : PubMed/NCBI
14	Polack PO, Guillemain I, Hu E, Deransart C, Depaulis A and Charpier S: Deep layer somatosensory cortical neurons initiate spike-and-wave discharges in a genetic model of absence seizures. J Neurosci. 27:6590–6599. 2007. View Article : Google Scholar
15	Mylvaganam S, Zhang L, Wu C, et al: Hippocampal seizures alter the expression of the pannexin and connexin transcriptome. J Neurochem. 112:92–102. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Altered expression of pannexin proteins in patients with temporal lobe epilepsy

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Related Articles