Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro

Yu,Bohan; Wang,Zuolin

doi:10.3892/mmr.2013.1756

Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro

Authors:
- Bohan Yu
- Zuolin Wang
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Affiliations: Center of Implant Dentistry, Hospital of Stomatology, Tongji University, Shanghai 200092, P.R. China
Published online on: October 24, 2013 https://doi.org/10.3892/mmr.2013.1756
Pages: 235-242
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Identifying a reliable and effective cytokine or growth factor group has been the focus of stem cell osteogenic induction studies. Concentrated growth factors (CGFs) as the novel generation of platelet concentrate products, appear to exhibit a superior clinical and biotechnological application potential, however, there are few studies that have demonstrated this effect. This study investigated the proliferation and differentiation of periodontal ligament stem cells (PDLSCs) co‑cultured with CGFs. The rate of proliferation was analyzed by cell counting and an MTT assay. Mineralization nodule counts, alkaline phosphatase activity detection, qPCR, western blot analysis and immunohistochemistry were used to analyze mineralization effects. The results showed that CGF significantly promoted the proliferation of PDLSCs, and exhibited a dose‑dependent effect on the activation and differentiation of the stem cells. The application of CGF on PDLSC proliferation and osteoinduction may offer numerous clinical and biotechnological application strategies.

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