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Article

Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19

  • Authors:
    • Xiu-Ran Wang
    • Guang-Mou Yan
    • Rui Zhang
    • Xu-Long Lang
    • Yan‑Ling Yang
    • Xiao-Yan Li
    • Si Chen
    • Jing Qian
    • Xing-Long Wang
  • View Affiliations / Copyright

    Affiliations: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, P.R. China, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, AMMS, Changchun 130122, P.R. China, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130122, P.R. China
  • Pages: 653-658
    |
    Published online on: November 18, 2013
       https://doi.org/10.3892/mmr.2013.1810
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Abstract

Brucellosis is an infectious disease affecting humans and animals worldwide. Effective methods of control include inducing immunity in animals by vaccination and elimination. Brucella abortus S19 is one of the popular vaccines for control of cattle brucellosis, as it has low virulence. In this paper, allelic exchange plasmids of wzm and wzt genes were constructed and partially knocked out to evaluate the effects on the induction of immunity to Brucella abortus S19 mutants. Cytokine secretion in vitro, INF-γ induction in vivo and antibody dynamics were evaluated. These data suggested that the immunity-eliciting ability of the wzm and wzt gene deletion mutants was similar, although reduced compared with the S19 strain. The results demonstrated that the wzt gene may be more important in the regulation of the induction of immunity than the wzm gene.
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1 

Cardoso PG, Macedo GC, Azevedo V and Oliveira SC: Brucella spp noncanonical LPS: structure, biosynthesis and interaction with host immune system. Microb Cell Fact. 5:132006. View Article : Google Scholar

2 

Rambow-Larsen AA, Petersen EM, Gourley CR and Splitter GA: Brucella regulators: self-control in hostil environment. Trends Microbiol. 17:371–377. 2009. View Article : Google Scholar

3 

Al-Tawfiq JA: Therapeutic options for human brucellosis. Expert Rev Anti Infect Ther. 6:109–120. 2008. View Article : Google Scholar

4 

Pappas G, Papadimitrious P, Akritidis N, Christou L and Tsianos E: The new global map of human brucellosis. Lancet Infect Dis. 6:91–99. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Ficht TA, Kahl-McDonagh MM, Arenas-Gamboa AM and Rice-Ficht AC: Brucellosis: the case for live, attenuated vaccines. Vaccine. 27:D40–D43. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Spink WW, Hall JW, Finstad J and Mallet E: Immunization with viable Brucella organisms. Results of a safety test in humans. B World Health Organ. 26:409–419. 1962.

7 

Perkins SD, Smither SJ and Atkins HS: Towards a Brucella vaccine for humans. FEMS Microbiol Rev. 34:379–394. 2010.

8 

Monreal D, Grilló MJ, González D, Marín CM, De Miguel MJ, López-Goni I, Blasco JM, Cloeckaert A and Moriyón I: Characterization of Brucella abortus O-polysaccharide and core lipopolysaccharide mutant and demonstration that a complete core is required for rough vaccines to be efficient against Brucella abortus and Brucella ovis in the mouse model. Infect Immun. 71:3261–3271. 2003.

9 

Fugier E, Pappas G and Gorvel JP: Virulence factors in brucellosis: implications for aetiopathogenesis and treatment. Expert Rev Mol Med. 9:1–10. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Bundle DR, Cherwonogrodzky JW, Gidney MA, Meikle PJ, Perry MB and Peters T: Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides. Infect Immun. 57:2829–2836. 1989.PubMed/NCBI

11 

Weynants V, Gilson D, Cloeckaert A, Tibor A, Denoel PA, Godfroid F, Limet JN and Letesson JJ: Characterization of smooth lipopolysaccharides and O-polysaccharides of Brucella species by competition binding assays with monoclonal antibodies. Infect Immun. 65:1939–1943. 1997.PubMed/NCBI

12 

Ugalde JE, Comerci DJ, Leguizamón MS and Ugalde RA: Evaluation of Brucella abortus phosphoglucomutase (pgm) mutant as a new live rough-phenotype vaccine. Infect Immun. 71:6264–6269. 2003.PubMed/NCBI

13 

Lapaque N, Moriyon I, Moreno E and Gorvel JP: Brucella lipopolysaccharide acts as a virulence factor. Curr Opin Microbiol. 8:60–66. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Eisenschenk FC, Houle JJ and Hoffmann EM: Serum sensitivity of field isolates and laboratory strains of Brucella abortus. Am J Vet Res. 56:1592–1598. 1995.PubMed/NCBI

15 

Velasco J, Bengoechae JA, Brandenberg K, Lindner B, Seydel U, Gonzalez D, Zahringer U, Moreno E and Moriyon I: Brucella abortus and its closest phylogenetic relative, ochrobacterum spp, differ in outer membrane permeability and cationic peptide resistance. Infect Immun. 68:3210–3218. 2000. View Article : Google Scholar

16 

Lapaque N, Forquet F, de Chastellier C, Mishal Z, Jolly G, Moreno E, Moriyon I, Heuser JE, He HT and Gorvel JP: Characterization of Brucella abortus lipopolysaccharide macrodomains as mega rafts. Cell Microbiol. 8:197–206. 2006.

17 

Porte F, Naroeni A, Ouahrani-Bettache S and Liautard JP: Role of the Brucella suis lipopolysaccharide O antigen in phagosomal genesis and in inhibition of phagosome-lysosome fusion in murine macrophages. Infect Immun. 74:1481–1490. 2003.

18 

Fernandez-Prada CM, Zelazowska EB, Nikolich M, Hadfield TL, Roop RM, Robertson GL and Hoover DL: Interactions between Brucella melitensis and human phagocytes: bacterial surface O-polysaccharide inhibits phagocytosis, bacterial killing and subsequent host cell apoptosis. Infect Immun. 71:2110–2119. 2003.

19 

Jiménez de Bagüés MP, Terraza A, Gross A and Dornand J: Different responses of macrophages to smooth and rough Brucella spp: relationship to virulence. Infect Immun. 72:2429–2433. 2004.PubMed/NCBI

20 

Moriyón I, Grilló MJ, Monreal D, González D, Marín C, López-Goñi I, Mainar-Jaime RC, Moreno E and Blasco JM: Rough vaccines in animals brucellosis: structural and genetic basis and present status. Vet Res. 35:1–38. 2004.PubMed/NCBI

21 

Adone R, Ciuchini F, Marianelli C, Tarantino M, Pistoia C, Marcon G, Petrucci P, Francia M, Riccardi G and Pasquali P: Protective properties of rifampin-resistant rough mutants of Brucella melitensis. Infect Immun. 73:4198–4204. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Haag AF, Myka KK, Arnold MF, Caro–Hernández P and Ferguson GP: Importance of lipopolysaccharide and cyclic β-1,2-glucans in Brucella-mammalian infections. Int J Microbiol. 2010:1245092010.

23 

Cloeckaert A, Grayon M, Verger JM, Letesson JJ and Godfroid F: Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp. Res Microbiol. 151:209–216. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Godfroid F, Cloeckaert A, Taminiau B, Danese I, Tibor A, de Bolle X, Mertens P and Letesson JJ: Genetic organization of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk). Res Microbiol. 151:655–668. 2000. View Article : Google Scholar : PubMed/NCBI

25 

González D, Grilló MJ, De Miguel MJ, Ali T, Arce-Gorvel V, Delrue RM, Conde-Alvarez R, Muñoz P, López-Goñi I, Iriarte M, Marín CM, Weintraub A, Widmalm G, Zygmunt M, Letesson JJ, Gorvel JP, Blasco JM and Moriyón I: Brucellosis vaccines: assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export. PLoS One. 3:e27602008.

26 

Allen CA, Adams LG and Ficht TA: Transposon-derived Brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival. Infect Immun. 66:1008–1016. 1998.PubMed/NCBI

27 

Ried JL and Collmer A: An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis. Gene. 57:239–246. 1987. View Article : Google Scholar : PubMed/NCBI

28 

Christian RHR and Chris W: Lipopolysaccharide endotoxins. Annu Rev Biochem. 71:635–700. 2002. View Article : Google Scholar

29 

Grilló MJ, Blasco JM, Gorvel JP, Moriyón I and Moreno E: What have we learned from brucellosis in the mouse model? Vet Res. 43:292012.PubMed/NCBI

30 

Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA and Pascual DW: Murine and bovine γδT cells enhance innate immunity against Brucella abortus infections. PLoS One. 6:e219782012.

31 

Li X and He Y: Caspase-2-dependent dendritic cell death, maturation and priming of t cells in response to Brucella abortus infection. PLoS One. 7:e435122012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Yan G, Zhang R, Lang X, Yang YL, Li X, Chen S, Qian J and Wang X: Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19. Mol Med Rep 9: 653-658, 2014.
APA
Wang, X., Yan, G., Zhang, R., Lang, X., Yang, Y., Li, X. ... Wang, X. (2014). Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19. Molecular Medicine Reports, 9, 653-658. https://doi.org/10.3892/mmr.2013.1810
MLA
Wang, X., Yan, G., Zhang, R., Lang, X., Yang, Y., Li, X., Chen, S., Qian, J., Wang, X."Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19". Molecular Medicine Reports 9.2 (2014): 653-658.
Chicago
Wang, X., Yan, G., Zhang, R., Lang, X., Yang, Y., Li, X., Chen, S., Qian, J., Wang, X."Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19". Molecular Medicine Reports 9, no. 2 (2014): 653-658. https://doi.org/10.3892/mmr.2013.1810
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Yan G, Zhang R, Lang X, Yang YL, Li X, Chen S, Qian J and Wang X: Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19. Mol Med Rep 9: 653-658, 2014.
APA
Wang, X., Yan, G., Zhang, R., Lang, X., Yang, Y., Li, X. ... Wang, X. (2014). Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19. Molecular Medicine Reports, 9, 653-658. https://doi.org/10.3892/mmr.2013.1810
MLA
Wang, X., Yan, G., Zhang, R., Lang, X., Yang, Y., Li, X., Chen, S., Qian, J., Wang, X."Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19". Molecular Medicine Reports 9.2 (2014): 653-658.
Chicago
Wang, X., Yan, G., Zhang, R., Lang, X., Yang, Y., Li, X., Chen, S., Qian, J., Wang, X."Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19". Molecular Medicine Reports 9, no. 2 (2014): 653-658. https://doi.org/10.3892/mmr.2013.1810
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