Effects of recombinant human interleukin‑10 on Treg cells, IL‑10 and TGF‑β in transplantation of rabbit skin

Liu,Kai  Shan; Fan,Xiao  Qin; Zhang,Lei; Wen,Qiong  Na; Feng,Ji  Hong; Chen,Fu  Chao; Luo,Jun  Min; Sun,Wan  Bang

doi:10.3892/mmr.2013.1817

2014-February Volume 9 Issue 2

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2014-February Volume 9 Issue 2

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Article Open Access

Effects of recombinant human interleukin‑10 on Treg cells, IL‑10 and TGF‑β in transplantation of rabbit skin

Authors:
- Kai Shan Liu
- Xiao Qin Fan
- Lei Zhang
- Qiong Na Wen
- Ji Hong Feng
- Fu Chao Chen
- Jun Min Luo
- Wan Bang Sun
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Affiliations: The Border Armed Police Central Hospital of Guangdong Province, Shenzhen, Guangdong 518023, P.R. China, College of Life Science and Teaching, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Basic Teaching, Zunyi Medical College, Zhuhai Campus, Zhuhai, Guizhou 519041, P.R. China, Department of Respiratory Diseases, Central People's Hospital of Zhanjiang, Zhanjiang, Guangdong 524037, P.R. China, Department of Surgical Laboratory, Zunyi Medical College Zhuhai Campus, Zhuhai, Guizhou 519041, P.R. China, Department of Immunology, Institute of Basic Medical Science, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China

Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 639-644
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Published online on: November 20, 2013

https://doi.org/10.3892/mmr.2013.1817
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Abstract

The current study aimed to investigate the rejection and survival time of grafted skin, and the changes of Treg cells, interleukin 10 (IL‑10) and transforming growth factor‑β (TGF‑β) in peripheral blood following skin transplantation with recombinant human interleukin‑10 (rhIL‑10) or cyclosporin A (CsA), as well as the role of IL‑10 in immunological rejection mechanisms. A total of 36 rabbits were divided into two groups. The skin of a donor rabbit was transplanted onto the back of one receptor rabbit. Receptors were randomly divided into six groups, including rhIL‑10 low‑dose (5 µg/kg/d), rhIL‑10 high‑dose (10 µg/kg/d), CsA low‑dose (5 mg/kg/d), CsA high‑dose (10 mg/kg/d), rhIL‑10 (5 µg/kg/d) and CsA (5 mg/kg/d) and negative control normal saline (NS; 1 ml/d). All groups received intramuscular drug injection for ten days, beginning one day prior to skin transplantation surgery. Following transplantation, each rabbit's peripheral blood was collected at different times. The changes of CD4+CD25+ regulatory T cells, IL‑10 and TGF‑β were determined by flow cytometry and enzyme‑linked immunosorbent assay. When compared with the control group, the rejection and survival times of the experimental groups were longer following skin graft. Compared with the two CsA groups and the control group, the proportion of CD4+CD25+ regulatory T cells of rhIL‑10 groups was significantly upregulated on the 4th and 7th days following surgery. However, TGF‑β levels were not significantly different. Data suggested that the concentration of IL‑10 was positively correlated with the proportion of CD4+CD25+ regulatory T cells. In addition, IL‑10 may delay the rejection time of rabbit skin transplantation and prolong the survival time. Thus, the role of IL‑10 in inhibited allograft rejection may be associated with CD4+CD25+ regulatory T cells and IL‑10, and may be independent of TGF‑β.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of recombinant human interleukin‑10 on Treg cells, IL‑10 and TGF‑β in transplantation of rabbit skin

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