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Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice

  • Authors:
    • Hao Li
    • Yong Qiang
    • Lian Wang
    • Canhui Liu
    • Nan Yang
    • Lei Xiong
    • Jun Yi
    • Hua Jing
    • Haiwei Wu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Jinling Hospital, Clinical Medicine School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 427-434
    |
    Published online on: November 28, 2013
       https://doi.org/10.3892/mmr.2013.1833
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Abstract

Previous studies have shown that lipopolysaccharide (LPS) induces acute lung injury (ALI), and that endothelial progenitor cells (EPCs) participate in tissue repair. Therefore, in this study it was hypothesized that LPS influences the number and function of EPCs directly. In order to investigate this, an in vitro study was performed using EPCs. EPCs were cultured for seven days (early EPCs), and then treated with increasing concentrations of LPS (10 pg/ml, 100 pg/ml, 1 ng/ml, 10 ng/ml and 100 ng/ml) for 4, 8, 12, and 24 h. The proliferation, senescence and adhesion of EPCs was then assessed. Alongside this an in vivo study was also performed. Mice were administered LPS (2.5 mg/kg) via the trachea. After 4, 8, 12, and 24 h, EPCs were harvested and cultured for seven days, and the proliferation, senescence and adhesion of the EPCs were examined. The results showed that the rate of adhesion and senescence of EPCs decreased in vitro when treated with 10 and 100 ng/ml LPS. The adhesion and senescence rate also decreased after 12 and 24 h in vivo. Proliferation, however, was increased in vitro following treatment with 10 and 100 ng/ml LPS, but proliferation in vivo decreased after 8 and 12 h. The effects of LPS on EPCs were distinct in vivo and in vitro. In vitro, cells were sensitive to 100 ng/ml LPS. In the course of ALI induced by LPS, the proliferation and adhesion activity of the EPCs was activated in 8 h and then gradually decreased with time.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Qiang Y, Wang L, Liu C, Yang N, Xiong L, Yi J, Jing H and Wu H: Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice. Mol Med Rep 9: 427-434, 2014.
APA
Li, H., Qiang, Y., Wang, L., Liu, C., Yang, N., Xiong, L. ... Wu, H. (2014). Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice. Molecular Medicine Reports, 9, 427-434. https://doi.org/10.3892/mmr.2013.1833
MLA
Li, H., Qiang, Y., Wang, L., Liu, C., Yang, N., Xiong, L., Yi, J., Jing, H., Wu, H."Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice". Molecular Medicine Reports 9.2 (2014): 427-434.
Chicago
Li, H., Qiang, Y., Wang, L., Liu, C., Yang, N., Xiong, L., Yi, J., Jing, H., Wu, H."Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice". Molecular Medicine Reports 9, no. 2 (2014): 427-434. https://doi.org/10.3892/mmr.2013.1833
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Qiang Y, Wang L, Liu C, Yang N, Xiong L, Yi J, Jing H and Wu H: Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice. Mol Med Rep 9: 427-434, 2014.
APA
Li, H., Qiang, Y., Wang, L., Liu, C., Yang, N., Xiong, L. ... Wu, H. (2014). Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice. Molecular Medicine Reports, 9, 427-434. https://doi.org/10.3892/mmr.2013.1833
MLA
Li, H., Qiang, Y., Wang, L., Liu, C., Yang, N., Xiong, L., Yi, J., Jing, H., Wu, H."Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice". Molecular Medicine Reports 9.2 (2014): 427-434.
Chicago
Li, H., Qiang, Y., Wang, L., Liu, C., Yang, N., Xiong, L., Yi, J., Jing, H., Wu, H."Effect of lipopolysaccharide on the characteristics of endothelial progenitor cells from bone marrow in mice". Molecular Medicine Reports 9, no. 2 (2014): 427-434. https://doi.org/10.3892/mmr.2013.1833
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