Carnosol inhibits cell adhesion molecules and chemokine expression by tumor necrosis factor-α in human umbilical vein endothelial cells through the nuclear factor-κB and mitogen-activated protein kinase pathways

  • Authors:
    • Hui Yao
    • Yufeng Chen
    • Longjuan Zhang
    • Xiaosheng He
    • Xiaowen He
    • Lei Lian
    • Xiaojian Wu
    • Ping Lan
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  • Published online on: December 3, 2013     https://doi.org/10.3892/mmr.2013.1839
  • Pages: 476-480
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Abstract

Inflammatory bowel diseases (IBD) are gastrointestinal disorders associated with chronic inflammatory processes. Carnosol has been demonstrated to possess anti-inflammatory properties. This study examined the suppressive effect of carnosol on the expression of cell adhesion molecules (CAMs) and chemokines in human umbilical vein endothelial cells (HUVECs) and the possible underlying mechanism. The effect of carnosol on CAM and chemokine expression in HUVECs was identified by western blotting and ELISA, respectively. nuclear factor (NF)-κB activation of HUVECs was analyzed using the TransAM NF-κB Family kit. The effect of carnosol on the tumor necrosis factor (TNF)-α-induced activation of the NF-κB and mitogen-activated protein kinase (MAPK) pathways, and was subsequently analyzed using western blotting. Carnosol not only inhibited TNF-α-induced protein expression of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1 and E-selectin in HUVECs, but also suppressed interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 expression. In addition, carnosol inhibited the TNF-α-induced phosphorylation of p-65 and IκB-α, as well as the activation of NF-κB. The same result was observed in TNF-α-stimulated phosphorylation of ERK1/2 and p-38. It was demonstrated that carnosol inhibited TNF-α-induced CAM and chemokine expression in HUVECs. The underlying mechanism may be associated with the blocking of the NF-κB and MAPK pathways. These results indicate that carnosol may be a novel therapeutic agent for targeting endothelial cells in IBDs.
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2014-February
Volume 9 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yao H, Chen Y, Zhang L, He X, He X, Lian L, Wu X and Lan P: Carnosol inhibits cell adhesion molecules and chemokine expression by tumor necrosis factor-α in human umbilical vein endothelial cells through the nuclear factor-κB and mitogen-activated protein kinase pathways. Mol Med Rep 9: 476-480, 2014.
APA
Yao, H., Chen, Y., Zhang, L., He, X., He, X., Lian, L. ... Lan, P. (2014). Carnosol inhibits cell adhesion molecules and chemokine expression by tumor necrosis factor-α in human umbilical vein endothelial cells through the nuclear factor-κB and mitogen-activated protein kinase pathways. Molecular Medicine Reports, 9, 476-480. https://doi.org/10.3892/mmr.2013.1839
MLA
Yao, H., Chen, Y., Zhang, L., He, X., He, X., Lian, L., Wu, X., Lan, P."Carnosol inhibits cell adhesion molecules and chemokine expression by tumor necrosis factor-α in human umbilical vein endothelial cells through the nuclear factor-κB and mitogen-activated protein kinase pathways". Molecular Medicine Reports 9.2 (2014): 476-480.
Chicago
Yao, H., Chen, Y., Zhang, L., He, X., He, X., Lian, L., Wu, X., Lan, P."Carnosol inhibits cell adhesion molecules and chemokine expression by tumor necrosis factor-α in human umbilical vein endothelial cells through the nuclear factor-κB and mitogen-activated protein kinase pathways". Molecular Medicine Reports 9, no. 2 (2014): 476-480. https://doi.org/10.3892/mmr.2013.1839