Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038

He,Ke; Si,Picheng; Wang,Hanning; Tahir,Usman; Chen,Kaiyun; Xiao,Jinfeng; Duan,Xiaopeng; Huang,Rui; Xiang,Guoan

doi:10.3892/mmr.2013.1851

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Corrigendum in: /10.3892/mmr.2023.13038

Authors:
- Ke He
- Picheng Si
- Hanning Wang
- Usman Tahir
- Kaiyun Chen
- Jinfeng Xiao
- Xiaopeng Duan
- Rui Huang
- Guoan Xiang
View Affiliations

Affiliations: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China, Department of General Surgery, People's Hospital of Heyuan, Heyuan, Guangdong 517001, P.R. China, College of Animal Science and Technology, China Agricultural University, Beijing 10083, P.R. China
Published online on: December 9, 2013 https://doi.org/10.3892/mmr.2013.1851
Pages: 521-526

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Gastric cancer (GC) is one of the most common types of gastrointestinal tumors worldwide, and the side effects of chemotherapeutic drugs and the resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe and effective novel agents are urgently required. The purpose of the present study was to investigate the crocetin‑sensitive treatment of GC and its possible mechanisms. BGC‑823 human GC cells were treated with crocetin. The effects of crocetin on the viability of the cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 dyeing and Rh123 staining were used to detect cell apoptosis. The BGC‑823 cells were subjected to western blotting analysis for detection of cytochrome c and cleaved caspase‑3 protein expression. Crocetin inhibited the proliferation of the GC cell line in a dose‑ and time‑dependent manner. Apoptotic BGC‑823 cells induced by crocetin were stained by Hoechst 33258 and observed under a light microscope for cell membrane staining of dense nuclei, nuclear pyknosis, fragmentation, chromatin condensation and highlighted nuclear membrane staining. This revealed a decline in the mitochondrial membrane potential of the BGC‑823 cells. Crocetin also induced caspase‑3 activation and cytochrome c translocation into the cytosol from the mitochondria. The results of this study indicate that crocetin induces the apoptosis of BGC‑823 cells, and may be used as an effective agent in the treatment of GC.

View Figures

View References

1	Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI
2	Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar
3	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar
4	Sun X, Mu R, Zhou YS, et al: Analysis of mortality rate of stomach cancer and its trend in twenty years in China. Zhonghua Zhong Liu Za Zhi. 26:4–9. 2004.(In Chinese).
5	Wagner AD, Grothe W, Haerting J, et al: Chemotherapy in advanced gastric cancer: a systematic review and meta-analysis based on aggregate data. J Clin Oncol. 24:2903–2909. 2006. View Article : Google Scholar : PubMed/NCBI
6	Ji YB, Gao SY, Ji CF and Zou X: Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein. J Ethnopharmacol. 115:194–202. 2008. View Article : Google Scholar : PubMed/NCBI
7	Majewska A, Hoser G, Furmanowa M, Urbańska N, Pietrosiuk A, Zobel A and Kuraś M: Antiproliferative and antimitotic effect, S phase accumulation and induction of apoptosis and necrosis after treatment of extract from Rhodiola rosea rhizomes on HL-60 cells. J Ethnopharmacol. 103:43–52. 2006. View Article : Google Scholar : PubMed/NCBI
8	Zhong L, Qu G, Li P, Han J and Guo D: Induction of apoptosis and G2/M cell cycle arrest by Gleditsioside E from Gleditsia sinensis in HL-60 cells. Planta Med. 69:561–563. 2003. View Article : Google Scholar : PubMed/NCBI
9	Zhong L, Li P, Han J, Qu G and Guo D: Structure-activity relationships of saponins from Gleditsia sinensis in cytotoxicity and induction of apoptosis. Planta Med. 70:797–802. 2004. View Article : Google Scholar : PubMed/NCBI
10	Abdullaev FI and Espinosa-Aguirre JJ: Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev. 28:426–432. 2004. View Article : Google Scholar : PubMed/NCBI
11	Das I, Das S and Saha T: Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: A histopathological study. Acta Histochem. 112:317–327. 2010. View Article : Google Scholar : PubMed/NCBI
12	Amin A, Hamza AA, Bajbouj K, Ashraf SS and Daoud S: Saffron: a potential candidate for a novel anticancer drug against hepatocellular cancer. Hepatology. 54:857–867. 2011. View Article : Google Scholar : PubMed/NCBI
13	Abdullaev Jafarova F, Caballero-Ortega H, Riverón-Negrete L, et al: In vitro evaluation of the chemopreventive potential of saffron. Rev Invest Clin. 54:430–436. 2002.(In Spanish).
14	Dhar A, Mehta S, Dhar G, et al: Crocetin inhibits pancreatic cancer cell proliferation and tumor progression in a xenograft mouse model. Mol Cancer Ther. 8:315–323. 2009. View Article : Google Scholar : PubMed/NCBI
15	Chryssanthi D, Dedes PG, Karamanos NK, Cordopatis P and Lamari FN: Crocetin inhibits invasiveness of MDA-MB-231 breast cancer cells via downregulation of matrix metalloproteinases. Planta Med. 77:146–151. 2011. View Article : Google Scholar : PubMed/NCBI
16	Wu Y, Chen Y, Qu R, Lan T and Sang J: Type II cGMP-dependent protein kinase inhibits EGF-triggered signal transduction of the MAPK/ERK-mediated pathway in gastric cancer cells. Oncol Rep. 27:553–558. 2012.PubMed/NCBI
17	Yang Y, Hu Y, Gu HY, et al: Oroxylin A induces G2/M phase cell-cycle arrest via inhibiting Cdk7-mediated expression of Cdc2/p34 in human gastric carcinoma BGC-823 cells. J Pharm Pharmacol. 60:1459–1463. 2008. View Article : Google Scholar : PubMed/NCBI
18	Zou Z, Xie L, Wei J, et al: Synergistic anti-proliferative effects of gambogic acid with docetaxel in gastrointestinal cancer cell lines. BMC Complement Altern Med. 12:582012. View Article : Google Scholar : PubMed/NCBI
19	Luo T, Jiang W, Kong Y, Li S, He F, Xu J and Wang HQ: The protective effects of jatrorrhizine on β-amyloid (25–35)-induced neurotoxicity in rat cortical neurons. CNS Neurol Disord Drug Targets. 11:1030–1037. 2012.
20	Elmore S: Apoptosis: a review of programmed cell death. Toxicol Pathol. 35:495–516. 2007. View Article : Google Scholar : PubMed/NCBI
21	Amin AR, Kucuk O, Khuri FR and Shin DM: Perspectives for cancer prevention with natural compounds. J Clin Oncol. 27:2712–2725. 2009. View Article : Google Scholar : PubMed/NCBI
22	Verrier F, Deniaud A, Lebras M, et al: Dynamic evolution of the adenine nucleotide translocase interactome during chemotherapy-induced apoptosis. Oncogene. 23:8049–8064. 2004. View Article : Google Scholar : PubMed/NCBI
23	Nair SC, Panikkar KR and Parthod RK: Protective effects of crocetin on the bladder toxicity induced by cyclophosphamide. Cancer Biother. 8:339–343. 1993. View Article : Google Scholar : PubMed/NCBI
24	Ferri KF and Kroemer G: Organelle-specific initiation of cell death pathways. Nat Cell Biol. 3:E255–E263. 2001. View Article : Google Scholar : PubMed/NCBI
25	Slee EA, Harte MT, Kluck RM, et al: Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspase-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J Cell Biol. 144:281–292. 1999.PubMed/NCBI
26	Porter AG and Jänicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI
27	Jänicke RU, Sprengart ML, Wati MR and Porter AG: Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J Biol Chem. 273:9357–9360. 1998.
28	Candé C, Cecconi F, Dessen P and Kroemer G: Apoptosis-inducing factor (AIF): key to the conserved caspase-independent pathways of cell death? J Cell Sci. 115:4727–4734. 2002.PubMed/NCBI
29	Guoan X, Hanning W, Kaiyun C and Hao L: Adenovirus-mediated siRNA targeting Mcl-1 gene increases radiosensitivity of pancreatic carcinoma cells in vitro and in vivo. Surgery. 147:553–561. 2010. View Article : Google Scholar : PubMed/NCBI
30	Halder J, Landen CN Jr, Lutgendorf SK, et al: Focal adhesion kinase silencing augments docetaxel-mediated apoptosis in ovarian cancer cells. Clin Cancer Res. 11:8829–8836. 2005. View Article : Google Scholar : PubMed/NCBI
31	Manu KA, Shanmugam MK, Ramachandran L, et al: First evidence that γ-tocotrienol inhibits the growth of human gastric cancer and chemosensitizes it to capecitabine in a xenograft mouse model through the modulation of NF-κB pathway. Clin Cancer Res. 18:2220–2229. 2012.
32	Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T and Yang H: Magnolol, a natural compound, induces apoptosis of SGC-7901 human gastric adenocarcinoma cells via the mitochondrial and PI3K/Akt signaling pathways. Int J Oncol. 40:1153–1161. 2012.PubMed/NCBI
33	Cousin FJ, Jouan-Lanhouet S, Dimanche-Boitrel MT, Corcos L and Jan G: Milk fermented by Propionibacterium freudenreichii induces apoptosis of HGT-1 human gastric cancer cells. PLoS One. 7:e318922012.PubMed/NCBI
34	Li CJ, Chu CY, Huang LH, Wang MH, Sheu LF, Yeh JI and Hsu HY: Synergistic anticancer activity of triptolide combined with cisplatin enhances apoptosis in gastric cancer in vitro and in vivo. Cancer Lett. 319:203–213. 2012. View Article : Google Scholar : PubMed/NCBI
35	Nam KN, Park YM, Jung HJ, et al: Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur J Pharmacol. 648:110–116. 2010. View Article : Google Scholar : PubMed/NCBI
36	Hamid A, Qian ZY, Song L and Yang R: Crocetin ameliorates TNBS induced chronic colitis in rats by inhibiting expression of Cyclooxygenase-2. J Nat Rem. 12:12–19. 2012.
37	Ohno Y, Nakanishi T, Umigai N, Tsuruma K, Shimazawa M and Hara H: Oral administration of crocetin prevents inner retinal damage induced by N-methyl-D-aspartate in mice. Eur J Pharmacol. 690:84–89. 2012. View Article : Google Scholar : PubMed/NCBI
38	Wang Y, Yan J, Xi L, Qian Z, Wang Z and Yang L: Protective effect of crocetin on hemorrhagic shock-induced acute renal failure in rats. Shock. 38:63–67. 2012. View Article : Google Scholar : PubMed/NCBI
39	Reuter S, Gupta SC, Chaturvedi MM and Aggarwal BB: Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med. 49:1603–1616. 2010. View Article : Google Scholar : PubMed/NCBI
40	Tye H, Kennedy CL, Najdovska M, et al: STAT3-driven upregulation of TLR2 promotes gastric tumorigenesis independent of tumor inflammation. Cancer Cell. 22:466–478. 2012. View Article : Google Scholar : PubMed/NCBI