Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
2014-February Volume 9 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-February Volume 9 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Corrigendum in: /10.3892/mmr.2023.13038
  • Authors:
    • Ke He
    • Picheng Si
    • Hanning Wang
    • Usman Tahir
    • Kaiyun Chen
    • Jinfeng Xiao
    • Xiaopeng Duan
    • Rui Huang
    • Guoan Xiang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second People's Hospital of Guangdong Province, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China, Department of General Surgery, People's Hospital of Heyuan, Heyuan, Guangdong 517001, P.R. China, College of Animal Science and Technology, China Agricultural University, Beijing 10083, P.R. China
  • Pages: 521-526
    |
    Published online on: December 9, 2013
       https://doi.org/10.3892/mmr.2013.1851
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Gastric cancer (GC) is one of the most common types of gastrointestinal tumors worldwide, and the side effects of chemotherapeutic drugs and the resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe and effective novel agents are urgently required. The purpose of the present study was to investigate the crocetin‑sensitive treatment of GC and its possible mechanisms. BGC‑823 human GC cells were treated with crocetin. The effects of crocetin on the viability of the cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 dyeing and Rh123 staining were used to detect cell apoptosis. The BGC‑823 cells were subjected to western blotting analysis for detection of cytochrome c and cleaved caspase‑3 protein expression. Crocetin inhibited the proliferation of the GC cell line in a dose‑ and time‑dependent manner. Apoptotic BGC‑823 cells induced by crocetin were stained by Hoechst 33258 and observed under a light microscope for cell membrane staining of dense nuclei, nuclear pyknosis, fragmentation, chromatin condensation and highlighted nuclear membrane staining. This revealed a decline in the mitochondrial membrane potential of the BGC‑823 cells. Crocetin also induced caspase‑3 activation and cytochrome c translocation into the cytosol from the mitochondria. The results of this study indicate that crocetin induces the apoptosis of BGC‑823 cells, and may be used as an effective agent in the treatment of GC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar

3 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

4 

Sun X, Mu R, Zhou YS, et al: Analysis of mortality rate of stomach cancer and its trend in twenty years in China. Zhonghua Zhong Liu Za Zhi. 26:4–9. 2004.(In Chinese).

5 

Wagner AD, Grothe W, Haerting J, et al: Chemotherapy in advanced gastric cancer: a systematic review and meta-analysis based on aggregate data. J Clin Oncol. 24:2903–2909. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Ji YB, Gao SY, Ji CF and Zou X: Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein. J Ethnopharmacol. 115:194–202. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Majewska A, Hoser G, Furmanowa M, Urbańska N, Pietrosiuk A, Zobel A and Kuraś M: Antiproliferative and antimitotic effect, S phase accumulation and induction of apoptosis and necrosis after treatment of extract from Rhodiola rosea rhizomes on HL-60 cells. J Ethnopharmacol. 103:43–52. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Zhong L, Qu G, Li P, Han J and Guo D: Induction of apoptosis and G2/M cell cycle arrest by Gleditsioside E from Gleditsia sinensis in HL-60 cells. Planta Med. 69:561–563. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Zhong L, Li P, Han J, Qu G and Guo D: Structure-activity relationships of saponins from Gleditsia sinensis in cytotoxicity and induction of apoptosis. Planta Med. 70:797–802. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Abdullaev FI and Espinosa-Aguirre JJ: Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev. 28:426–432. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Das I, Das S and Saha T: Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: A histopathological study. Acta Histochem. 112:317–327. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Amin A, Hamza AA, Bajbouj K, Ashraf SS and Daoud S: Saffron: a potential candidate for a novel anticancer drug against hepatocellular cancer. Hepatology. 54:857–867. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Abdullaev Jafarova F, Caballero-Ortega H, Riverón-Negrete L, et al: In vitro evaluation of the chemopreventive potential of saffron. Rev Invest Clin. 54:430–436. 2002.(In Spanish).

14 

Dhar A, Mehta S, Dhar G, et al: Crocetin inhibits pancreatic cancer cell proliferation and tumor progression in a xenograft mouse model. Mol Cancer Ther. 8:315–323. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Chryssanthi D, Dedes PG, Karamanos NK, Cordopatis P and Lamari FN: Crocetin inhibits invasiveness of MDA-MB-231 breast cancer cells via downregulation of matrix metalloproteinases. Planta Med. 77:146–151. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Wu Y, Chen Y, Qu R, Lan T and Sang J: Type II cGMP-dependent protein kinase inhibits EGF-triggered signal transduction of the MAPK/ERK-mediated pathway in gastric cancer cells. Oncol Rep. 27:553–558. 2012.PubMed/NCBI

17 

Yang Y, Hu Y, Gu HY, et al: Oroxylin A induces G2/M phase cell-cycle arrest via inhibiting Cdk7-mediated expression of Cdc2/p34 in human gastric carcinoma BGC-823 cells. J Pharm Pharmacol. 60:1459–1463. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Zou Z, Xie L, Wei J, et al: Synergistic anti-proliferative effects of gambogic acid with docetaxel in gastrointestinal cancer cell lines. BMC Complement Altern Med. 12:582012. View Article : Google Scholar : PubMed/NCBI

19 

Luo T, Jiang W, Kong Y, Li S, He F, Xu J and Wang HQ: The protective effects of jatrorrhizine on β-amyloid (25–35)-induced neurotoxicity in rat cortical neurons. CNS Neurol Disord Drug Targets. 11:1030–1037. 2012.

20 

Elmore S: Apoptosis: a review of programmed cell death. Toxicol Pathol. 35:495–516. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Amin AR, Kucuk O, Khuri FR and Shin DM: Perspectives for cancer prevention with natural compounds. J Clin Oncol. 27:2712–2725. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Verrier F, Deniaud A, Lebras M, et al: Dynamic evolution of the adenine nucleotide translocase interactome during chemotherapy-induced apoptosis. Oncogene. 23:8049–8064. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Nair SC, Panikkar KR and Parthod RK: Protective effects of crocetin on the bladder toxicity induced by cyclophosphamide. Cancer Biother. 8:339–343. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Ferri KF and Kroemer G: Organelle-specific initiation of cell death pathways. Nat Cell Biol. 3:E255–E263. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Slee EA, Harte MT, Kluck RM, et al: Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspase-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J Cell Biol. 144:281–292. 1999.PubMed/NCBI

26 

Porter AG and Jänicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Jänicke RU, Sprengart ML, Wati MR and Porter AG: Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J Biol Chem. 273:9357–9360. 1998.

28 

Candé C, Cecconi F, Dessen P and Kroemer G: Apoptosis-inducing factor (AIF): key to the conserved caspase-independent pathways of cell death? J Cell Sci. 115:4727–4734. 2002.PubMed/NCBI

29 

Guoan X, Hanning W, Kaiyun C and Hao L: Adenovirus-mediated siRNA targeting Mcl-1 gene increases radiosensitivity of pancreatic carcinoma cells in vitro and in vivo. Surgery. 147:553–561. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Halder J, Landen CN Jr, Lutgendorf SK, et al: Focal adhesion kinase silencing augments docetaxel-mediated apoptosis in ovarian cancer cells. Clin Cancer Res. 11:8829–8836. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Manu KA, Shanmugam MK, Ramachandran L, et al: First evidence that γ-tocotrienol inhibits the growth of human gastric cancer and chemosensitizes it to capecitabine in a xenograft mouse model through the modulation of NF-κB pathway. Clin Cancer Res. 18:2220–2229. 2012.

32 

Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T and Yang H: Magnolol, a natural compound, induces apoptosis of SGC-7901 human gastric adenocarcinoma cells via the mitochondrial and PI3K/Akt signaling pathways. Int J Oncol. 40:1153–1161. 2012.PubMed/NCBI

33 

Cousin FJ, Jouan-Lanhouet S, Dimanche-Boitrel MT, Corcos L and Jan G: Milk fermented by Propionibacterium freudenreichii induces apoptosis of HGT-1 human gastric cancer cells. PLoS One. 7:e318922012.PubMed/NCBI

34 

Li CJ, Chu CY, Huang LH, Wang MH, Sheu LF, Yeh JI and Hsu HY: Synergistic anticancer activity of triptolide combined with cisplatin enhances apoptosis in gastric cancer in vitro and in vivo. Cancer Lett. 319:203–213. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Nam KN, Park YM, Jung HJ, et al: Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur J Pharmacol. 648:110–116. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Hamid A, Qian ZY, Song L and Yang R: Crocetin ameliorates TNBS induced chronic colitis in rats by inhibiting expression of Cyclooxygenase-2. J Nat Rem. 12:12–19. 2012.

37 

Ohno Y, Nakanishi T, Umigai N, Tsuruma K, Shimazawa M and Hara H: Oral administration of crocetin prevents inner retinal damage induced by N-methyl-D-aspartate in mice. Eur J Pharmacol. 690:84–89. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Wang Y, Yan J, Xi L, Qian Z, Wang Z and Yang L: Protective effect of crocetin on hemorrhagic shock-induced acute renal failure in rats. Shock. 38:63–67. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Reuter S, Gupta SC, Chaturvedi MM and Aggarwal BB: Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med. 49:1603–1616. 2010. View Article : Google Scholar : PubMed/NCBI

40 

Tye H, Kennedy CL, Najdovska M, et al: STAT3-driven upregulation of TLR2 promotes gastric tumorigenesis independent of tumor inflammation. Cancer Cell. 22:466–478. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
He K, Si P, Wang H, Tahir U, Chen K, Xiao J, Duan X, Huang R and Xiang G: Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038. Mol Med Rep 9: 521-526, 2014.
APA
He, K., Si, P., Wang, H., Tahir, U., Chen, K., Xiao, J. ... Xiang, G. (2014). Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038. Molecular Medicine Reports, 9, 521-526. https://doi.org/10.3892/mmr.2013.1851
MLA
He, K., Si, P., Wang, H., Tahir, U., Chen, K., Xiao, J., Duan, X., Huang, R., Xiang, G."Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038". Molecular Medicine Reports 9.2 (2014): 521-526.
Chicago
He, K., Si, P., Wang, H., Tahir, U., Chen, K., Xiao, J., Duan, X., Huang, R., Xiang, G."Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038". Molecular Medicine Reports 9, no. 2 (2014): 521-526. https://doi.org/10.3892/mmr.2013.1851
Copy and paste a formatted citation
x
Spandidos Publications style
He K, Si P, Wang H, Tahir U, Chen K, Xiao J, Duan X, Huang R and Xiang G: Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038. Mol Med Rep 9: 521-526, 2014.
APA
He, K., Si, P., Wang, H., Tahir, U., Chen, K., Xiao, J. ... Xiang, G. (2014). Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038. Molecular Medicine Reports, 9, 521-526. https://doi.org/10.3892/mmr.2013.1851
MLA
He, K., Si, P., Wang, H., Tahir, U., Chen, K., Xiao, J., Duan, X., Huang, R., Xiang, G."Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038". Molecular Medicine Reports 9.2 (2014): 521-526.
Chicago
He, K., Si, P., Wang, H., Tahir, U., Chen, K., Xiao, J., Duan, X., Huang, R., Xiang, G."Crocetin induces apoptosis of BGC-823 human gastric cancer cells Corrigendum in /10.3892/mmr.2023.13038". Molecular Medicine Reports 9, no. 2 (2014): 521-526. https://doi.org/10.3892/mmr.2013.1851
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team