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Article

β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen

  • Authors:
    • Jaewoong Jang
    • Jong-Hyeok Ha
    • Seok-Min Kim
    • Wonyong Kim
    • Kijeong Kim
    • Sang-In Chung
    • Yoosik Yoon
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, School of Medicine, Chung‑Ang University, Seoul 156-756, Republic of Korea, School of Mechanical Engineering, Chung‑Ang University, Seoul 156-756, Republic of Korea
  • Pages: 633-638
    |
    Published online on: December 10, 2013
       https://doi.org/10.3892/mmr.2013.1852
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Abstract

The modulations of β-catenin were analyzed during the inflammatory response induced by the Der p 1 house dust mite allergen. Der p 1 induced the dose-dependent expression of inflammatory cytokines, including interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) in THP-1 human monocytic cells. The mRNA expression levels of β-catenin were not altered, however protein levels increased following Der p 1 treatment, demonstrating that β-catenin was modulated by post-transcriptional processes. It was also revealed that nuclear β-catenin levels were significantly increased while cytoplasmic β-catenin levels were reduced, which demonstrated the nuclear translocation of β-catenin by the Der p 1 allergen. Glycogen synthase kinase 3β (GSK3β), a regulator of β-catenin stability, was demonstrated to be phosphorylated following Der p 1 treatment. When β-catenin was knocked down by the transfection of its small interfering RNA (siRNA), inflammatory cytokine expression as well as nuclear factor-κB (NF-κB) activity, which were induced by Der p 1 treatment, were all significantly reduced. The results demonstrated that Der p 1-induced inflammatory responses were mediated by β-catenin.
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Copy and paste a formatted citation
Spandidos Publications style
Jang J, Ha J, Kim S, Kim W, Kim K, Chung S and Yoon Y: β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen. Mol Med Rep 9: 633-638, 2014.
APA
Jang, J., Ha, J., Kim, S., Kim, W., Kim, K., Chung, S., & Yoon, Y. (2014). β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen. Molecular Medicine Reports, 9, 633-638. https://doi.org/10.3892/mmr.2013.1852
MLA
Jang, J., Ha, J., Kim, S., Kim, W., Kim, K., Chung, S., Yoon, Y."β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen". Molecular Medicine Reports 9.2 (2014): 633-638.
Chicago
Jang, J., Ha, J., Kim, S., Kim, W., Kim, K., Chung, S., Yoon, Y."β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen". Molecular Medicine Reports 9, no. 2 (2014): 633-638. https://doi.org/10.3892/mmr.2013.1852
Copy and paste a formatted citation
x
Spandidos Publications style
Jang J, Ha J, Kim S, Kim W, Kim K, Chung S and Yoon Y: β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen. Mol Med Rep 9: 633-638, 2014.
APA
Jang, J., Ha, J., Kim, S., Kim, W., Kim, K., Chung, S., & Yoon, Y. (2014). β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen. Molecular Medicine Reports, 9, 633-638. https://doi.org/10.3892/mmr.2013.1852
MLA
Jang, J., Ha, J., Kim, S., Kim, W., Kim, K., Chung, S., Yoon, Y."β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen". Molecular Medicine Reports 9.2 (2014): 633-638.
Chicago
Jang, J., Ha, J., Kim, S., Kim, W., Kim, K., Chung, S., Yoon, Y."β-catenin mediates the inflammatory cytokine expression induced by the Der p 1 house dust mite allergen". Molecular Medicine Reports 9, no. 2 (2014): 633-638. https://doi.org/10.3892/mmr.2013.1852
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