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Article

Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway

  • Authors:
    • Shen Zhao
    • Qingming Lin
    • Heng Li
    • Yumin He
    • Xiangshao Fang
    • Feng Chen
    • Changwei Chen
    • Zitong Huang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China, Provincial Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Taiping People's Hospital of Dongguan City, Dongguan, Guangdong 523900, P.R. China
  • Pages: 754-762
    |
    Published online on: December 13, 2013
       https://doi.org/10.3892/mmr.2013.1861
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Abstract

Carbon monoxide (CO) is an endogenous gaseous transmitter that exerts multi‑protection in ischemia/reperfusion (I/R) injury, but few experimental studies regarding CO on myocardial I/R‑induced apoptosis, as well as its underlying mechanism have been conducted. The present study was designed to investigate whether CO released from CO‑releasing molecule‑2 (CORM‑2) is capable of ameliorating myocardial I/R‑induced apoptosis via a mitochondrial apoptotic pathway. Primary cultures of neonatal rat cardiomyocytes were randomly distributed into four groups: Control, I/R (cultured cardiomyocytes were subjected to 2 h simulated ischemia followed by 4 h reperfusion), CORM‑2 and inactive CORM‑2 (iCORM‑2) groups (20 µM CORM‑2 and 20 µM iCORM‑2 were administered at the beginning of reperfusion following ischemia, respectively). Flow cytometric analysis showed that CORM‑2 treatment significantly decreased apoptosis of cardiomyocytes triggered by simulated I/R. CORM‑2 partially recovered mitochondrial respiration and ultrastructure alteration, and lowered caspase‑3 expression and the release of cytochrome c. Furthermore, CORM‑2 partly reduced BAK/BAX expression in mitochondria, as well as the BAX level in the cytoplasm. Cardioprotection is lost when CORM‑2 is replaced by iCORM‑2. CORM‑2 treatment, at the time of reperfusion, was concluded to attenuate myocardial I/R‑induced apoptosis. The protection mechanisms may be targeted to the mitochondria and involved in the inhibition of the BAK/BAX‑mediated intrinsic pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao S, Lin Q, Li H, He Y, Fang X, Chen F, Chen C and Huang Z: Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway. Mol Med Rep 9: 754-762, 2014.
APA
Zhao, S., Lin, Q., Li, H., He, Y., Fang, X., Chen, F. ... Huang, Z. (2014). Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway. Molecular Medicine Reports, 9, 754-762. https://doi.org/10.3892/mmr.2013.1861
MLA
Zhao, S., Lin, Q., Li, H., He, Y., Fang, X., Chen, F., Chen, C., Huang, Z."Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway". Molecular Medicine Reports 9.2 (2014): 754-762.
Chicago
Zhao, S., Lin, Q., Li, H., He, Y., Fang, X., Chen, F., Chen, C., Huang, Z."Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway". Molecular Medicine Reports 9, no. 2 (2014): 754-762. https://doi.org/10.3892/mmr.2013.1861
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao S, Lin Q, Li H, He Y, Fang X, Chen F, Chen C and Huang Z: Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway. Mol Med Rep 9: 754-762, 2014.
APA
Zhao, S., Lin, Q., Li, H., He, Y., Fang, X., Chen, F. ... Huang, Z. (2014). Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway. Molecular Medicine Reports, 9, 754-762. https://doi.org/10.3892/mmr.2013.1861
MLA
Zhao, S., Lin, Q., Li, H., He, Y., Fang, X., Chen, F., Chen, C., Huang, Z."Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway". Molecular Medicine Reports 9.2 (2014): 754-762.
Chicago
Zhao, S., Lin, Q., Li, H., He, Y., Fang, X., Chen, F., Chen, C., Huang, Z."Carbon monoxide releasing molecule‑2 attenuated ischemia/reperfusion‑induced apoptosis in cardiomyocytes via a mitochondrial pathway". Molecular Medicine Reports 9, no. 2 (2014): 754-762. https://doi.org/10.3892/mmr.2013.1861
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