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Article Open Access

Effect of dexmedetomidine on lung ischemia‑reperfusion injury

  • Authors:
    • Lili Jiang
    • Li Li
    • Jinmei Shen
    • Zeyou Qi
    • Liang Guo
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Second Xiang‑Ya Hospital, Xiang‑Ya Medical College, Central South University, Changsha, Hunan 410011, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 419-426
    |
    Published online on: December 17, 2013
       https://doi.org/10.3892/mmr.2013.1867
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Abstract

Dexmedetomidine, a specific selective α2‑adrenergic agonist, does not only have the characteristics of being a sedative and analgesic, but also exhibits a protective role in brain ischemia‑reperfusion injury and inhibits the inflammation in animals with sepsis. The objective of the present study was to investigate whether dexmedetomidine is capable of attenuating rat pulmonary damage induced by ischemia‑reperfusion injury, which is a type of acute sterile lung injury. Sprague‑Dawley rats were randomly assigned into six groups: The sham‑operated (sham) group, the lung ischemia‑reperfusion (I/R) group, intravenous injection of dexmedetomidine 2.5 µg/kg/h (Dex2.5) or 5 µg/kg/h (Dex5) for 1 h prior to ischemia, combination of α2‑adrenergic antagonist yohimbine prior to dexmedetomidine pre‑treatment (Dex+Yoh) and pre‑administration of yohimbine alone (Yoh) prior to ischemia. Lung injury was assessed by the histopathological changes, arterial blood gas, wet/dry (w/d) weight ratio and myeloperoxidase (MPO) activity of the lung. The concentration of tumor necrosis factor‑α (TNF‑α), interleukin‑6 (IL‑6) and monocyte chemoattractant protein‑1 (MCP‑1) in bronchoalveolar lavage fluid (BALF) was measured by an enzyme-linked immunosorbent assay. The expression of toll‑like receptor‑4 (TLR4) and myeloid differentiation factor 88 (MyD88) mRNA in the lung were determined by quantitative PCR, and phosphorylated levels of c‑Jun N‑terminal kinase (JNK) and extracellular signal‑regulated kinase (ERK)1/2 were determined by western blotting. Pre‑treatment with dexmedetomidine significantly reduced the lung injury, w/d weight ratio and MPO activity, and decreased the concentration of TNF‑α, IL‑6 and MCP‑1 in BALF compared with the I/R group. The expression of TLR4 and MyD88 mRNA and the levels of phosphorylated JNK and ERK1/2 in the lung tissue were markedly downregulated by intravenous injection of dexmedetomidne for 1 h prior to lung I/R. The protective effects of dexmedetomidine on the lung were not completely reversed by the α2‑adrenergic antagonist, yohimbine. Pre‑treatment with dexmedetomidine is capable of reducing pulmonary damage and inhibiting sterile inflammation induced by lung I/R injury. TLR4/MyD88/mitogen-activated protein kinase (MAPK) signaling is involved in the protective mechanism of dexmedetomidine through α2‑adrenoceptor independence.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Li L, Shen J, Qi Z and Guo L: Effect of dexmedetomidine on lung ischemia‑reperfusion injury. Mol Med Rep 9: 419-426, 2014.
APA
Jiang, L., Li, L., Shen, J., Qi, Z., & Guo, L. (2014). Effect of dexmedetomidine on lung ischemia‑reperfusion injury. Molecular Medicine Reports, 9, 419-426. https://doi.org/10.3892/mmr.2013.1867
MLA
Jiang, L., Li, L., Shen, J., Qi, Z., Guo, L."Effect of dexmedetomidine on lung ischemia‑reperfusion injury". Molecular Medicine Reports 9.2 (2014): 419-426.
Chicago
Jiang, L., Li, L., Shen, J., Qi, Z., Guo, L."Effect of dexmedetomidine on lung ischemia‑reperfusion injury". Molecular Medicine Reports 9, no. 2 (2014): 419-426. https://doi.org/10.3892/mmr.2013.1867
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L, Li L, Shen J, Qi Z and Guo L: Effect of dexmedetomidine on lung ischemia‑reperfusion injury. Mol Med Rep 9: 419-426, 2014.
APA
Jiang, L., Li, L., Shen, J., Qi, Z., & Guo, L. (2014). Effect of dexmedetomidine on lung ischemia‑reperfusion injury. Molecular Medicine Reports, 9, 419-426. https://doi.org/10.3892/mmr.2013.1867
MLA
Jiang, L., Li, L., Shen, J., Qi, Z., Guo, L."Effect of dexmedetomidine on lung ischemia‑reperfusion injury". Molecular Medicine Reports 9.2 (2014): 419-426.
Chicago
Jiang, L., Li, L., Shen, J., Qi, Z., Guo, L."Effect of dexmedetomidine on lung ischemia‑reperfusion injury". Molecular Medicine Reports 9, no. 2 (2014): 419-426. https://doi.org/10.3892/mmr.2013.1867
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