Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
2014-March Volume 9 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-March Volume 9 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells

  • Authors:
    • Benjamin Ngee Tiing Law
    • Anna Pick Kiong Ling
    • Rhun Yian Koh
    • Soi Moi Chye
    • Ying Pei Wong
  • View Affiliations / Copyright

    Affiliations: School of Medical Sciences, Faculty of Medicine and Health, International Medical University, Kuala Lumpur 57000, Malaysia, Department of Human Biology, Faculty of Medicine and Health, International Medical University, Kuala Lumpur 57000, Malaysia
  • Pages: 947-954
    |
    Published online on: December 24, 2013
       https://doi.org/10.3892/mmr.2013.1878
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Neurodegenerative diseases remain a global issue which affects the ageing population. Efforts towards determining their aetiologies to understand their pathogenic mechanisms are underway in order to identify a pathway through which therapeutic measures can be applied. One such pathogenic mechanism, oxidative stress (OS), is widely considered to be involved in neurodegenerative disease. Antioxidants, most notably flavonoids, have promising potential for therapeutic use as shown in in vitro and in vivo studies. In view of the importance of flavonoids for combating OS, this study investigated the neuroprotective effects of orientin, which has been reported to be capable of crossing the blood‑brain barrier. The maximum non‑toxic dose (MNTD) of orientin against SH‑SY5Y neuroblastoma cells was determined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay. The effects of the MNTD and the half MNTD (½MNTD) of orientin on cell cycle progression and intracellular reactive oxygen species (ROS) levels, as well as the activity of caspases 3/7, 8 and 9 after exposure to 150 µM of hydrogen peroxide (H2O2) were also determined using flow cytometry, a 2',7'‑dichlorodihydrofluorescein‑diacetate (DCFH‑DA) assay and caspase assay kits, respectively. The results revealed that orientin at ≤20 µM was not cytotoxic to SH‑SY5Y cells. After treatment with orientin at the MNTD, the percentage of apoptotic cells was significantly reduced compared with that in cells treated with 150 µM H2O2 alone. The results also showed that, although orientin at the MNTD and ½MNTD did not reduce intracellular ROS levels, it significantly inhibited the activity of caspases 3/7. Caspase 9 was significantly inactivated with orientin at the MNTD. Findings from this study suggest that the neuroprotection conferred by orientin was the result of the intracellular mediation of caspase activity.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Kolominsky-Rabas PL, Sarti C, Heuschmann PU, Graf C, Siemonsen S, Neundoerfer B, Katalinic A, Lang E, Gassmann KG and von Stockert TR: A prospective community-based study of stroke in Germany - the Erlangen Stroke Project (ESPro): incidence and case fatality at 1, 3, and 12 months. Stroke. 29:2501–2506. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Samsa GP, Bian J, Lipscomb J and Matchar DB: Epidemiology of recurrent cerebral infarction: a medicare claims-based comparison of first and recurrent strokes on 2-year survival and cost. Stroke. 30:338–349. 1999.PubMed/NCBI

3 

Leppälä JM, Virtamo J, Fogelholm R, Albanes D and Heinonen OP: Different risk factors for different stroke subtypes: association of blood pressure, cholesterol, and antioxidants. Stroke. 30:2535–2540. 1999.PubMed/NCBI

4 

Mattson MP: Neuronal life-and-death signaling, apoptosis, and neurodegenerative disorders. Antioxid Redox Signal. 8:1997–2006. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Emerit J, Edeas M and Bricaire F: Neurodegenerative diseases and oxidative stress. Biomed Pharmacother. 58:39–46. 2004. View Article : Google Scholar

6 

Vrinda B and Uma Devi P: Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin. Mutat Res. 498:39–46. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Lu N, Sun Y and Zheng X: Orientin-induced cardioprotection against reperfusion is associated with attenuation of mitochondrial permeability transition. Planta Med. 77:984–991. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Pusztai R, Béládi I, Bakai M, Mucsi I and Kukán E: Study on the effect of flavonoids and related substances. I The effect of quercetin on different viruses. Acta Microbiol Acad Sci Hung. 13:113–118. 1966.PubMed/NCBI

9 

Korkina LG and Afanas’ev IB: Antioxidant and chelating properties of flavonoids. Adv Pharmacol. 38:151–163. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Matsuo M, Sasaki N, Saga K and Kaneko T: Cytotoxicity of flavonoids toward cultured normal human cells. Biol Pharm Bull. 28:253–259. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Hassan HM and Fridovich I: Regulation of the synthesis of superoxide dismutase in Escherichia coli. Induction by methyl viologen. J Biol Chem. 252:7667–7672. 1977.PubMed/NCBI

12 

Yoshpe-Purer Y, Henis Y and Yashpe J: Regulation of catalase level in Escherichia coli K12. Can J Microbiol. 23:84–91. 1977. View Article : Google Scholar

13 

An JH and Blackwell TK: SKN-1 links C. elegans mesendodermal specification to a conserved oxidative stress response. Genes Dev. 17:1882–1893. 2003.PubMed/NCBI

14 

Inoue H, Hisamoto N, An JH, Oliveira RP, Nishida E, Blackwell TK and Matsumoto K: The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response. Genes Dev. 19:2278–2283. 2005.

15 

Sablina AA, Budanov AV, Ilyinskaya GV, Agapova LS, Kravchenko JE and Churnakov PM: The antioxidant function of the p53 tumor suppressor. Nat Med. 11:1306–1313. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Foreman J, Demidchik V, Bothwell JH, Mylona P, Miedema H, Torres MA, Linstead P, Costa S, Brownlee C, Jones JD, et al: Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature. 422:442–446. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Geizst M and Leto TL: The Nox family of NAD(P)H oxidases: host defense and beyond. J Biol Chem. 279:51715–51718. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Li J, Stouffs M, Serrander L, Banfi B, Bettiol E, Charnay Y, Steger K, Krause KH and Jaconi ME: The NADPH oxidase NOX4 drives cardiac differentiation: Role in regulating cardiac transcription factors and MAP kinase activation. Mol Biol Cell. 17:3978–3988. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Sauer H, Rahimi G, Hescheler J and Wartenberg M: Role of reactive oxygen species and phosphatidylinositol 3-kinase in cardiomyocyte differentiation of embryonic stem cells. FEBS Lett. 476:218–223. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Ushio-Fukai M: Localizing NADPH oxidase-derived ROS. Sci STKE. 2006:re82006.PubMed/NCBI

21 

Cai H: Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences. Cardiovasc Res. 68:26–36. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Gechev TS and Hille J: Hydrogen peroxide as a signal controlling plant programmed cell death. J Cell Biol. 168:17–20. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Whittemore ER, Loo DT, Watt JA and Cotman CW: A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture. Neuroscience. 67:921–932. 1995. View Article : Google Scholar : PubMed/NCBI

24 

Satoh T, Sakai N, Enokido Y, Uchiyama Y and Hatanaka H: Free radical-independent protection by nerve growth factor and Bcl-2 of PC12 cells from hydrogen peroxide-triggered apoptosis. J Biochem. 120:540–546. 1996. View Article : Google Scholar

25 

Jacobson MD: Reactive oxygen species and programmed cell death. Trends Biochem Sci. 21:83–86. 1996. View Article : Google Scholar : PubMed/NCBI

26 

Uberti D, Piccioni L, Colzi A, Bravi D, Canonico PL and Memo M: Pergolide protects SH-SY5Y cells against neurodegeneration induced by H2O2. Eur J Pharmacol. 434:17–20. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Heo SR, Han AM, Kwon YK and Joung I: p62 protects SH-SY5Y neuroblastoma cells against H2O2-induced injury through the PDK1/Akt pathway. Neurosci Lett. 450:45–50. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Zhang L, Yu H, Sun Y, Lin X, Chen B, Tan C, Cao G and Wang Z: Protective effects of salidroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells. Eur J Pharmacol. 564:18–25. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Tarozzi A, Morroni F, Hrelia S, Angeloni C, Marchesi A, Cantelli-Forti G and Hrelia P: Neuroprotective effects of anthocyanins and their in vivo metabolites in SH-SY5Y cells. Neurosci Lett. 424:36–40. 2007. View Article : Google Scholar

30 

Kwon SH, Kim JA, Hong SI, Jung YH, Kim HC, Lee SY and Jang CG: Loganin protects against hydrogen peroxide-induced apoptosis by inhibiting phosphorylation of JNK, p38, and ERK 1/2 MAPKs in SH-SY5Y cells. Neurochem Int. 58:533–541. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Suematsu N, Hosoda M and Fujimori K: Protective effects of quercetin against hydrogen peroxide-induced apoptosis in human neuronal SH-SY5Y cells. Neurosci Lett. 504:223–227. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Elmore S: Apoptosis: a review of programmed cell death. Toxicol Pathol. 35:495–516. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Shoulson I: Neuroprotective clinical strategies for Parkinson’s disease. Ann Neurol. 32(Suppl): S143–S145. 1992.

34 

Li D, Wang Q, Yuan ZF, Zhang L, Xu L, Cui Y and Duan K: Pharmacokinetics and tissue distribution study of orientin in rat by liquid chromatography. J Pharm Biomed Anal. 47:429–434. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Schroeter H, Spencer JP, Rice-Evans C and Williams RJ: Flavonoids protect neurons from oxidized low-density-lipoprotein-induced apoptosis involving c-Jun N-terminal kinase (JNK), c-Jun and caspase-3. Biochem J. 358:547–557. 2001. View Article : Google Scholar

36 

Kang SS, Lee JY, Choi YK, Kim GS and Han BH: Neuroprotective effects of flavones on hydrogen peroxide-induced apoptosis in SH-SY5Y neuroblostoma cells. Bioorg Med Chem Lett. 14:2261–2264. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Wang CN, Chi CW, Lin YL, Chen CF and Shiao YJ: The neuroprotective effects of phytoestrogens on amyloid beta protein-induced toxicity are mediated by abrogating the activation of caspase cascade in rat cortical neurons. J Biol Chem. 276:5287–5295. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Woo M, Hakem R, Soengas MS, Duncan GS, Shahinian A, Kagi D, Hakem A, McCurrach M, Khoo W, Kaufman SA, et al: Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes. Genes Dev. 12:806–819. 1998. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Law BN, Ling AP, Koh RY, Chye SM and Wong YP: Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells. Mol Med Rep 9: 947-954, 2014.
APA
Law, B.N., Ling, A.P., Koh, R.Y., Chye, S.M., & Wong, Y.P. (2014). Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells. Molecular Medicine Reports, 9, 947-954. https://doi.org/10.3892/mmr.2013.1878
MLA
Law, B. N., Ling, A. P., Koh, R. Y., Chye, S. M., Wong, Y. P."Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells". Molecular Medicine Reports 9.3 (2014): 947-954.
Chicago
Law, B. N., Ling, A. P., Koh, R. Y., Chye, S. M., Wong, Y. P."Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells". Molecular Medicine Reports 9, no. 3 (2014): 947-954. https://doi.org/10.3892/mmr.2013.1878
Copy and paste a formatted citation
x
Spandidos Publications style
Law BN, Ling AP, Koh RY, Chye SM and Wong YP: Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells. Mol Med Rep 9: 947-954, 2014.
APA
Law, B.N., Ling, A.P., Koh, R.Y., Chye, S.M., & Wong, Y.P. (2014). Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells. Molecular Medicine Reports, 9, 947-954. https://doi.org/10.3892/mmr.2013.1878
MLA
Law, B. N., Ling, A. P., Koh, R. Y., Chye, S. M., Wong, Y. P."Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells". Molecular Medicine Reports 9.3 (2014): 947-954.
Chicago
Law, B. N., Ling, A. P., Koh, R. Y., Chye, S. M., Wong, Y. P."Neuroprotective effects of orientin on hydrogen peroxide‑induced apoptosis in SH‑SY5Y cells". Molecular Medicine Reports 9, no. 3 (2014): 947-954. https://doi.org/10.3892/mmr.2013.1878
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team